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Tian M, Mochizuki K, Loidl J (2019) Non-coding RNA Transcription in Tetrahymena Meiotic Nuclei Requires Dedicated Mediator Complex-Associated Proteins. Current biology : CB ( ): PUBMED:31280995
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Identifiers and Description
Gene Model Identifier
TTHERM_01243450
Standard Name
OGL1 (OGG1-Like)
Aliases
PreTt01756 | 287.m00027
Description
HhH-GPD superfamily base excision DNA repair protein
Genome Browser (Macronucleus)
Genome Browser (Micronucleus)
Gene Ontology Annotations
Molecular Function
oxidized purine nucleobase lesion DNA N-glycosylase activity (IEA) | GO:0008534
OGG1 Mitochondrial glycosylase
/lyase that specifically excis
es 7,8-dihydro-8-oxoguanine re
sidues located opposite cytosi
ne or thymine residues in DNA,
repairs oxidative damage to m
itochondrial DNA, contributes
to UVA resistance
Tetrahymena thermophila’s OGL1 protein is homologous to proteins from the HhH-GPD and OGG-N superfamilies, which are associated with DNA repair. It contains two domains; the first domain (E-value= 3.0 -17), in the HhH-GPD family, is a Helix-hairpin-helix and Gly/Pro rich loop, found on proteins that remove base lesions. The second domain (E-value=6.9-13), is common to oxoguanine gylcosylases, also known as OGG1, which removes 8-oxoG lesions. The closest homologs are in Scheffersomyces stipitis, a negative Crabtree yeast, and in Taphrina deformans, a fungi/plant pathogen, with 46% identical residues. OGL1 may be an essential protein in preventing mutations in DNA that lead to a short lifespan, early aging, and cancer.
Paragraph by: Lillian Horin, Maite Cortes Garcia, Francis Ryu, Forrest Fulgenzi, Keck Science Center, Pitzer College