Gene Model Identifier
TTHERM_00865050
Standard Name
ORC1
Aliases
PreTt29135 | 154.m00093 | 3751.m00439
Description
Largest subunit of the origin recognition complex
Genome Browser (Macronucleus)
Genome Browser (Micronucleus)
No Data fetched for Gene Ontology Annotations
No Data fetched for Domains
Tetrahymena Functional Genomics Database:
Signal Peptide Present?: No
TTHERM_00865050
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No Data fetched for StockID
(v.2006 protein sequences)
Source Identifier Score Description
Tetrahymena borealis EI9_19673.1 9.997093137033517e-262 hypothetical protein (2343 aa)
Oxytricha Contig20978.0.g107 1.9997677415930956e-65 ATPase family associated with
DictyBase DDB_G0283073 9.995951036711152e-55 orcA on chromosome: 4 position
WormBase WBGene00012650 3.998097515166115e-39 locus:orc-1 status:Partially
SGD YML065W 1.0004783691736523e-35 ORC1 Largest subunit of the or
Stentor Coeruleus SteCoe_36947 9.602680054508676e-24
Paragraph No Gene Name Paragraph Text 61 ORC1 Gene Information:
TTHERM_00865050 is a homolog of annotated and functionally characterized Orc1 in other experimental organisms (24% amino acid sequence identity, 49% similarity to human Orc1). Orc1 is a component of the heterohexameric origin recognition complex (ORC) found to be involved in initiation of DNA replication. TtOrc1 has canonical Walker A and B motifs (involved in ATP binding and hydrolysis). TtORC is unusual in that it contains an integral RNA subunit (26T RNA) that binds to its cognate DNA target in the ribosomal DNA (rDNA) replication origin.
62 ORC1 Nucleic Acid Interactions (with the ORC complex):
26T RNA (5’-AUGUCUAAGUGUGAUGAUAAACGAAAAAAAAUAAAAAUUAA-3’).
Type I element T-rich strand: essential cis-acting replication determinant in ribosomal DNA origin of replication. (Location 5’ non-transcribed spacer)
(C3 rDNA type IB element T-rich strand, T51: 5’-CTCAAAAGTTGCAAAAGTTCGGAAGGTTTACTATTTTTGTTTTTTTTTT-3’). – requires 26T RNA and ATP
dsDNA – non rDNA chromosomes
63 ORC1 Protein Interaction Partners:
TtOrc2 (Co-migration on a native gel, detection by WB).
64 ORC1 Biochemical Activities (of ORC complex):
ATP binding, likely ATP hydrolysis
DNA binding - typeI element T-rich strand DNA
RNA binding – 26T RNA
65 ORC1 Regulation & Expression:
mRNA and protein: Cell cycle regulated
Maximal protein expression at G1/S border, degraded in S phase
Ref:19153611 : Donti TR, Datta S, Sandoval PY, Kapler GM (2009) Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins. The EMBO journal 28(3):223-33Ref:18007594 : Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60Ref:12832485 : Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17
>TTHERM_00865050(coding)ATG AAC ACA AGA AAT ACA TAA AGG TCA ATA GAT CCA AAA AAA GGA AAG TAG CAA TAA AAA TCA GTT AGC AAA AGT AGA AGC AAA AGC AAT AAT ATA ACT TAA AGC AAT GAA AAG ATA AAA AGG AGT TAA AGA TTA AAG TCA AAA GAT GAG ATG ATA GAT TCG GTT TAA GAA CCC AAT ATT TTC GTC TAG AAA GAT AAA AAT AAA CAA TAA TAA TCT AAA AGT AAA TCT AAG AAT TCA ACA AAA GAA TCA GAA AAA AAG CCT TAA AAA AGA GCA TTT AAA ATT GAG ATA CCT AAA TAA AAT GAA TAA AAA TTA AAA AAA ACT TAA GAA TCA AAT AAA ACA ATA ATA AAT TTG ACT AAA TAG TAG AAA TAA CAA TTA AAA TCA TAA TAG CTA ACA AAA GCT GTA ATT GAG TAA TAA ATA CAC ACT GAA TCA AAG AAA AGG GAA TAC AAT AAG CAA AAG AAT AAA AAT GAT AAT TTA GAA AAA ACT AAA AAT AAT CAT AAT ATT GTT ACT ATT GAT TCC AGC AAT GAT GAA AAT GAA AGT GAA TAT GAA ATT TCA GAT TGT CAT ATT TCA GTT TCT GTA TAT AAT AAT GAT AAT GAA ATA GAT ATA GAT AAA AAT GAA TAG TCT GTA GAT GTA AAG TAA AAT AAA AAT AAA AAT ATG ATA GAT AGT AGA GGA AGA AGT AAA AAT AGC CTT AAA ATT AAA AAA ACA TAT TCT TCT AGC TAG TAA AAG AGT TAA GAA AAA TAG TAA GTT AAA GAA TCA ACT GCC AGC AAA AGG ATT ACT CGT AGC CAA TAA AAT TTT GAA CTT ACA GAG TTA TAA GAT ACA AAG GCA AAA AGT GCT TCA AAA AAC AAA ACA ATA AAT TCG GAA AAG CAA AAT TAA AAA ATG AAT TCT AAA GAA AAA ATT GAT ACA AGC AAA GAC AAG AAT CCA TCA TTA ATT GAA AGT GAA AAA CCT AAA AGA GGA AGA AAG CCT AAA TAA GTA GAG ATA TTA AAT GAT AAA ATT TAA GAA AAA GAG ACA GAT TAG TAA AAA ACA CCT AAA AAG AGG GGG AGA AAA AGT AAA TAA GAA GCA TTG TTA GAT AGA AGA AAC ATT AAG GAG ATA TAA TAA TAA AAA GAG TAC GAA GAG AAA TAA TAA AAG AAA TCT TCA TCT AAA GAA TCT TAA AAA TTA ACT CAA GCC AGC GAA CAT TTT AAC TTT TAA GCG CTA ATT TAT TAA TAA AAA CCC GCC AAA AAA GAG TTA CGT ATT GAA GAC AAG AAT TAT TAA AAA ATA AAT GTT AAA CCA AAA CAT AAT ATT TAT GAT TAA GCT ATA GAA ATG CTA TAA GAA TAT TCA TTG CCA GAA GAA ATT CCT TGT AGA GAA GAT GAA AAG AAA TAA ATT TTA GAA TTT ATA AAT GAA GGT TTA GGC AAT AAT GGT AGC TCT AAT TGC TTG TAT ATT TCA GGT GTT CCT GGT ATA GGA AAG ACA GCA AGT TTT TTG GAA GTG ATT AAA AAA TTA TAA AAT GAA AAA AAA GAT GAA TTT ACT TTC ATT CAC ATT AAT GCT ATG AAT TTA AGT AAC CCA GAA AAT TTA TAC TAT ATA CTC GTT AAA ACA ATA ACT GGG AAA AAT TGC ACA TCA AAA TAA AAA GCC TGT TAA ATT TTA AAT GAG CTT TTC ACT CGT GGA AAA CTT TCA AAA ACA TAC TAA AGC TAT GGA GAA AAT ATT GAT AAT AAA AAT AAG GTG ATT CTC TTA GAT GAA TTA GAT TAT TTA GTC ACA TAA GAT TAG GAT TTA CTT TAT AAC TTA ATG GAA TGG CCT CAT CAT AAA TAC TCA AAA TTA ACT ATT ATT GGG ATA GCT AAT ACT ATG AAT TTA CCA GAA ATT TTG ATG AAC AAA ATT AAA AGT AGA ATG GGA AGT AGA AGA CTT GTT TTT AAT TAG TAT AAT CAT AAA TAA ATA CAG GAA ATT ATT GCT ACT AGA CTT AAA AAT TAG GAA AAA GTT AGG GAA GTG TTT GAG TAA AAT GCA ATA GAA TAT ACA TGC AGA AAA ATT GCT ATT TCA TCT TCA GAT ATA AGA AAA ACA TTG AAA GTG TTA AGA AAA GCT GTT GAA ATA TGT CAG TTA GAA AAC TTT TAA AAT TAA AAT GTT ACA AAA GTA ACT ATT CCT ATG ATT TAA AAA TCG TAT AGC TAG TTA TAC TCT TCT CCT ATT CTA TAT TCA ATT TAA AAA CTT TAA TTT CAT CAT AAG TTG ATG ATT CTT TCT ATA GCT TTA GAA AAT AAG CAC AGA GGA ATA CCA GTA GCT TAC TTG TCA GAG AGC TAT AAT AGA TAT AGA ATA ATG TTG AGC AAT CTA GGT AAA AAC GAA ACT ATT AAT TAT AAT CAA ATG AAA ATG GTC CTG AAC TAA CTT TCT GTT TTA AAT TTA ATA CAA TTG AAG GAA AAA GAA ATA GTT AAA GAT ACA CAA TTA ATT GGT TTA GAT CCT AAT AAG AAA CAC TTT TGT CAT GTT GTT GAC ATT TAA ATT AAT TCT AAT TTT AGT GTT GAT GAT GTC ACT AAC TCT CTA AAA GAA GAT GAA ATT TAT TTG AAA TTC TAA CAT TAA TTC TGA >TTHERM_00865050(protein)M N T R N T Q R S I D P K K G K Q Q Q K S V S K S R S K S N N I T Q S N E K I K R S Q R L K S K D E M I D S V Q E P N I F V Q K D K N K Q Q Q S K S K S K N S T K E S E K K P Q K R A F K I E I P K Q N E Q K L K K T Q E S N K T I I N L T K Q Q K Q Q L K S Q Q L T K A V I E Q Q I H T E S K K R E Y N K Q K N K N D N L E K T K N N H N I V T I D S S N D E N E S E Y E I S D C H I S V S V Y N N D N E I D I D K N E Q S V D V K Q N K N K N M I D S R G R S K N S L K I K K T Y S S S Q Q K S Q E K Q Q V K E S T A S K R I T R S Q Q N F E L T E L Q D T K A K S A S K N K T I N S E K Q N Q K M N S K E K I D T S K D K N P S L I E S E K P K R G R K P K Q V E I L N D K I Q E K E T D Q Q K T P K K R G R K S K Q E A L L D R R N I K E I Q Q Q K E Y E E K Q Q K K S S S K E S Q K L T Q A S E H F N F Q A L I Y Q Q K P A K K E L R I E D K N Y Q K I N V K P K H N I Y D Q A I E M L Q E Y S L P E E I P C R E D E K K Q I L E F I N E G L G N N G S S N C L Y I S G V P G I G K T A S F L E V I K K L Q N E K K D E F T F I H I N A M N L S N P E N L Y Y I L V K T I T G K N C T S K Q K A C Q I L N E L F T R G K L S K T Y Q S Y G E N I D N K N K V I L L D E L D Y L V T Q D Q D L L Y N L M E W P H H K Y S K L T I I G I A N T M N L P E I L M N K I K S R M G S R R L V F N Q Y N H K Q I Q E I I A T R L K N Q E K V R E V F E Q N A I E Y T C R K I A I S S S D I R K T L K V L R K A V E I C Q L E N F Q N Q N V T K V T I P M I Q K S Y S Q L Y S S P I L Y S I Q K L Q F H H K L M I L S I A L E N K H R G I P V A Y L S E S Y N R Y R I M L S N L G K N E T I N Y N Q M K M V L N Q L S V L N L I Q L K E K E I V K D T Q L I G L D P N K K H F C H V V D I Q I N S N F S V D D V T N S L K E D E I Y L K F Q H Q F >TTHERM_00865050(gene)ATG AAC ACA AGA AAT ACA TAA AGG TCA ATA GAT CCA AAA AAA GGA AAG TAG CAA TAA AAA TCA GTT AGC AAA AGT AGA AGC AAA AGC AAT AAT ATA ACT TAA AGC AAT GAA AAG ATA AAA AGG AGT TAA AGA TTA AAG TCA AAA GAT GAG ATG ATA GAT TCG GTT TAA GAA CCC AAT ATT TTC GTC TAG AAA GAT AAA AAT AAA CAA TAA TAA TCT AAA AGT AAA TCT AAG AAT TCA ACA AAA GAA TCA GAA AAA AAG CCT TAA AAA AGA GCA TTT AAA ATT GAG ATA CCT AAA TAA AAT GAA TAA AAA TTA AAA AAA ACT TAA GAA TCA AAT AAA ACA ATA ATA AAT TTG ACT AAA TAG TAG AAA TAA CAA TTA AAA TCA TAA TAG CTA ACA AAA GCT GTA ATT GAG TAA TAA ATA CAC ACT GAA TCA AAG AAA AGG GAA TAC AAT AAG CAA AAG AAT AAA AAT GAT AAT TTA GAA AAA ACT AAA AAT AAT CAT AAT ATT GTT ACT ATT GAT TCC AGC AAT GAT GAA AAT GAA AGT GAA TAT GAA ATT TCA GAT TGT CAT ATT TCA GTT TCT GTA TAT AAT AAT GAT AAT GAA ATA GAT ATA GAT AAA AAT GAA TAG TCT GTA GAT GTA AAG TAA AAT AAA AAT AAA AAT ATG ATA GAT AGT AGA GGA AGA AGT AAA AAT AGC CTT AAA ATT AAA AAA ACA TAT TCT TCT AGC TAG TAA AAG AGT TAA GAA AAA TAG TAA GTT AAA GAA TCA ACT GCC AGC AAA AGG ATT ACT CGT AGC CAA TAA AAT TTT GAA CTT ACA GAG TTA TAA GAT ACA AAG GCA AAA AGT GCT TCA AAA AAC AAA ACA ATA AAT TCG GAA AAG CAA AAT TAA AAA ATG AAT TCT AAA GAA AAA ATT GAT ACA AGC AAA GAC AAG AAT CCA TCA TTA ATT GAA AGT GAA AAA CCT AAA AGA GGA AGA AAG CCT AAA TAA GTA GAG ATA TTA AAT GAT AAA ATT TAA GAA AAA GAG ACA GAT TAG TAA AAA ACA CCT AAA AAG AGG GGG AGA AAA AGT AAA TAA GAA GCA TTG TTA GAT AGA AGA AAC ATT AAG GAG ATA TAA TAA TAA AAA GAG TAC GAA GAG AAA TAA TAA AAG AAA TCT TCA TCT AAA GAA TCT TAA AAA TTA ACT CAA GCC AGC GAA CAT TTT AAC TTT TAA GCG CTA ATT TAT TAA TAA AAA CCC GCC AAA AAA GAG TTA CGT ATT GAA GAC AAG AAT TAT TAA AAA ATA AAT GTT AAA CCA AAA CAT AAT ATT TAT GAT TAA GCT ATA GAA ATG CTA TAA GAA TAT TCA TTG CCA GAA GAA ATT CCT TGT AGA GAA GAT GAA AAG AAA TAA ATT TTA GAA TTT ATA AAT GAA GGT TTA GGC AAT AAT GGT AGC TCT AAT TGC TTG TGT AAT CAA TTA ATT TTA AAT TCT TTT TAA ATT TTC ATA AAA TTT AAA AAA ATT TAT TAA TCA TAA ATT AAA AAT TTA GAT ATT TCA GGT GTT CCT GGT ATA GGA AAG ACA GCA AGT TTT TTG GAA GTG ATT AAA AAA TTA TAA AAT GAA AAA AAA GAT GAA TTT ACT TTC ATT CAC ATT AAT GCT ATG AAT TTA AGT AAC CCA GAA AAT TTA TAC TAT ATA CTC GTT AAA ACA ATA ACT GGG AAA AAT TGC ACA TCA AAA TAA AAA GCC TGT TAA ATT TTA AGT AAG CAA TTA ATT TAT TAA TAC TTA AAA AAT TAA TTT ATT GTT TTA ATT TTA TTA GAT GAG CTT TTC ACT CGT GGA AAA CTT TCA AAA ACA TAC TAA AGC TAT GGA GAA AAT ATT GAT AAT AAA AAT AAG TAA TTT TTG ACT AAA AAA TAA TAA AAC TAT ATT TAA AAT ACT ATT TTA ATT AAA TTC AGG GTG ATT CTC TTA GAT GAA TTA GAT TAT TTA GTC ACA TAA GAT TAG GAT TTA CTT TAT AAC TTA ATG GAA TGG CCT CAT CAT AAA TAC TCA AAA TTA ACT ATT ATT GGG ATA GCT AAT ACT ATG AAT TTA CCA GAA ATT TTG ATG AAC AAA ATT AAA AGT AGA ATG GGA AGT AGA AGA CTT GTT TTT AAT TAG TAT AAT CAT AAA TAA ATA CAG GAA ATT ATT GCT ACT AGA CTT AAA AAT TAG GAA AAA GTT AGG GAA GTG TTT GAG TAA AAT GCA ATA GAA TAT ACA TGC AGA AAA ATT GCT ATT TCA TCT TCA GAT ATA AGA AAA ACA TTG AAA GTG TTA AGA AAA GCT GTT GAA ATA TGT CAG TTA GAA AAC TTT TAA AAT TAA AAT GTT ACA AAA GTA ACT ATT CCT ATG ATT TAA AAA TCG TAT AGC TAG TTA TAC TCT TCT CCT ATT CTA TAT TCA ATT TAA AAA CTT TAA TTT CAT CAT AAG TTG ATG ATT CTT TCT ATA GCT TTA GAA AAT AAG CAC AGA GGA ATA CCA GTA GCT TAC TTG TCA GAG GTA AAT ATT TAA ATT CTA AAT TTA TAT AAT TTT TAT TTA TAT ATT TAT GAT ATA GAG CTA TAA TAG ATA TAG AAT AAT GTT GAG CAA TCT AGG TAA AAA CGA AAC TAT TAA TTA TAA TCA AAT GAA AAT GGT CCT GAA CTA ACT TTC TGT TTT AAA TTT AAT ACA ATT GAA GGA AAA AGA AAT AGT TAA AGA TAC ACA ATT AAT TGG TTT AGA TCC TAA TAA GAA ACA CTT TTG TCA TGT TGT TGA CAT TTA AAT TAA TTC TAA TTT TAG TGT TGA TGA TGT CAC TAA CTC TCT AAA AGA AGA TGA AAT TTA TTT GAA ATT CTA ACA TTA ATT CTG A
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