Identifiers and Description

Gene Model Identifier

TTHERM_00675850

Standard Name

LIA3 (localized in macronuclear anlagen 3)

Aliases

PreTt11026 | 101.m00181

Description

a parallel G quadruplex-binding protein that regulates DNA elimination boundaries

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

• ( TIGR01784 ) conserved hypothetical protein

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.4%

TetraMine Data

TTHERM_00675850

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Stentor Coeruleus	SteCoe_20738	0
Tetrahymena borealis	EI9_14253.1	9.997093137033517e-262	hypothetical protein (405 aa)

General Information

Paragraph No	Gene Name	Paragraph Text
112	LIA3	In cells lacking LIA3 (ΔLIA3), the excision of IESs bounded by specific G-rich polypurine tracts was impaired and imprecise, whereas the removal of IESs without such controlling sequences was unaffected. Lia3p binds to oligonucleotides containing these polypurine tracts, which form parallel G-quadruplex structures in vitro.

Associated Literature

1. Ref:30918956: Lin CG, Chao JL, Tsai HK, Chalker D, Yao MC (2019) Setting boundaries for genome-wide heterochromatic DNA deletions through flanking inverted repeats in Tetrahymena thermophila. Nucleic acids research 47(10):5181-5192
2. Ref:31194876: Jaspan VN, Taye ME, Carle CM, Chung JJ, Chalker DL (2019) Boundaries of eliminated heterochromatin of Tetrahymena are positioned by the DNA-binding protein Ltl1. Nucleic acids research ( ):
3. Ref:26950070: Carle CM, Zaher HS, Chalker DL (2016) A Parallel G Quadruplex-Binding Protein Regulates the Boundaries of DNA Elimination Events of Tetrahymena thermophila. PLoS genetics 12(3):e1005842
4. Ref:17519286: Yao MC, Yao CH, Halasz LM, Fuller P, Rexer CH, Wang SH, Jain R, Coyne RS, Chalker DL (2007) Identification of novel chromatin-associated proteins involved in programmed genome rearrangements in Tetrahymena. Journal of cell science 120(Pt 12):1978-89

Sequences

>TTHERM_00675850(coding)
ATGAAATCTCATTCAAAGAAATACCAAGTAACAAATAATTTAGATGCTGACAATGAATTA
TATCCTCAATCACAAATTTAGAGTTCCAATAATGATTAGTAAACTCAACTGAACAGTCAA
GAAGAAAATAACCCTGATATGATTGAAACTGAAGATATAAACTTTAATCCAAATAACTCC
AATAAACCAACAAATAGTTCTTATAAAATTCAAACAATAAACTTAGAGAATAACAATCTA
GGTGAAAATACTTCGCAATAGACTTCAACAATTAAAAAAAATATCAATAGCAACAATTAG
AATTCTATTATAGAAACAACCTATTAGCAAGATAAATTCATAAATGGTGCAATAATTGCA
ATTAGGTCTCAAAATCTAGCTGTTAAGTATATTGTTGACCATATTAAGGAAATAACAGAC
AGAAATTTGTCTGAAGCTTATGTAAGAAAGATATGGGATCATTGGCTTAAATACAAAGAT
ATTTACTTCTCTTCAACACATAAAACAAGACTCTACACAAAGCATGAAGCCATTCAACTT
TATGATGCTATTTTTAAGCAATATAATCCAACTAACTAATCTTAAAGTATTATCCCTAAA
ATCAAAGAAGTATTGTAAAAACATGCTATAACAAAAAAATTGTTTATCAACTACTCACAA
TTCGAAGAAGTCAAAGGACTAGATACTGAAGAAACATATCTCTCAATTAAAGAAGCTATA
GACTCAGGTTAGTAATCTGATGAGCCAAAATGGATTGATGTCAGTGTAAGAATGATGATT
TCAGCAAGCTAGTTTGAATACAAGAAACAGATTGAAATTCAATGTTTTAATAAACTTAAA
AATTATTTGCAATTTTCTTGTATATAATTGGTTGGTGTAGTTATGGGTGAAACACTTAAT
GGAAGAGGAAAATTCATTTATATCCATTGTAGATATGATCAGTTAATACTAAAGAGGAAC
AAGTACTTGAAAAGATTCTTGCTTGAAGTTATTCCTGACTGGTTTTATTCTTATAACTGT
GTGCATAGTCAAAAAATTGCTATTTTTAAGTACTCTAAATTTTTATATCTCTAACGCAAG
ATACTTGAGGAGTAAGAAAGAAAAAGATTTATTCCTAATCCAGTCAATCCTCAGATAAAT
CAAACCAATCGTTTTAACAATTCTAGTGCTATAAATACCTCCCTTAACATTTCCTATCAA
TCATCTGCTGTTCCTAATACGAGTAGTATACCTCTGAACAGTTTATTCTGA

>TTHERM_00675850(protein)
MKSHSKKYQVTNNLDADNELYPQSQIQSSNNDQQTQLNSQEENNPDMIETEDINFNPNNS
NKPTNSSYKIQTINLENNNLGENTSQQTSTIKKNINSNNQNSIIETTYQQDKFINGAIIA
IRSQNLAVKYIVDHIKEITDRNLSEAYVRKIWDHWLKYKDIYFSSTHKTRLYTKHEAIQL
YDAIFKQYNPTNQSQSIIPKIKEVLQKHAITKKLFINYSQFEEVKGLDTEETYLSIKEAI
DSGQQSDEPKWIDVSVRMMISASQFEYKKQIEIQCFNKLKNYLQFSCIQLVGVVMGETLN
GRGKFIYIHCRYDQLILKRNKYLKRFLLEVIPDWFYSYNCVHSQKIAIFKYSKFLYLQRK
ILEEQERKRFIPNPVNPQINQTNRFNNSSAINTSLNISYQSSAVPNTSSIPLNSLF

>TTHERM_00675850(gene)
ATGAAATCTCATTCAAAGAAATACCAAGTAACAAATAATTTAGATGCTGACAATGAATTA
TATCCTCAATCACAAATTTAGAGTTCCAATAATGATTAGTAAACTCAACTGAACAGTCAA
GAAGAAAATAACCCTGATATGATTGAAACTGAAGATATAAACTTTAATCCAAATAACTCC
AATAAACCAACAAATAGTTCTTATAAAATTCAAACAATAAACTTAGAGAATAACAATCTA
GGTGAAAATACTTCGCAATAGACTTCAACAATTAAAAAAAATATCAATAGCAACAATTAG
AATTCTATTATAGAAACAACCTATTAGCAAGATAAATTCATAAATGGTGCAATAATTGCA
ATTAGGTCTCAAAATCTAGCTGTTAAGTATATTGTTGACCATATTAAGGAAATAACAGAC
AGAAATTTGTCTGAAGCTTATGTAAGAAAGATATGGGATCATTGGCTTAAATACAAAGAT
ATTTACTTCTCTTCAACACATAAAACAAGACTCTACACAAAGCATGAAGCCATTCAACTT
TATGATGCTATTTTTAAGCAATATAATCCAACTAACTAATCTTAAAGTATTATCCCTAAA
ATCAAAGAAGTAATTATCAAAAAATCTTATTTTAACTAAAAATTATATCCCTTTTGTTTT
TTTAAAACAATCTAGGTATTGTAAAAACATGCTATAACAAAAAAATTGTTTATCAACTAC
TCACAATTCGAAGAAGTCAAAGGACTAGATACTGAAGAAACATATCTCTCAATTAAAGAA
GCTATAGACTCAGGTTAGTAATCTGATGAGCCAAAATGGTATTTGCTTTTTATAATTTTT
ATCTATTTTAAGTGTTGTGTTTTTTTATGTTAAATTATTAAATAATTTTCATTTTAAATT
TAGGATTGATGTCAGTGTAAGAATGATGATTTCAGCAAGCTAGTTTGAATACAAGAAACA
GATTGAAATTCAATGTTTTAATAAACTTAAAAATTATTTGCAATTTTCTTGTATATAATT
GGTTGGTGTAGTTATGGGTGAAACACTTAATGGAAGAGGAAAATTCATTTATATCCATTG
TAGATATGATCAGTTAATACTAAAGAGGAACAAGTACTTGAAAAGATTCTTGCTTGAAGT
TATTCCTGACTGGTTTTATTCTTATAACTGTGTGCATAGTCAAAAAATTGCTATTTTTAA
GTACTCTAAATTTTTATATCTCTAACGCAAGATACTTGAGGAGTAAGAAAGAAAAAGATT
TATTCCTAATCCAGTCAATCCTCAGATAAATCAAACCAATCGTTTTAACAATTCTAGTGC
TATAAATACCTCCCTTAACATTTCCTATCAATCATCTGCTGTTCCTAATACGAGTAGTAT
ACCTCTGAACAGTTTATTCTGA