TGD | Gene Details

Identifiers and Description

Gene Model Identifier
TTHERM_00655890

Standard Name
NgoA (Non-GrOwth )

Aliases
PreTt03683 | 96.m00152

Description
NgoA

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Biological Process
conjugation with mutual genetic exchange (IEP) | GO:0000748 | Ref:6646127

Domains

No Data fetched for Domains

Gene Expression Profile

Tetrahymena Functional Genomics Database: TTHERM_00655890

Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

No Data fetched for Homologs

General Information

Paragraph No Gene Name Paragraph Text
9 HHO1,
CDC2,
NgoA,
CYP1 HHO1 knockouts show no global increase or decrease in the amount of transcription in the cell; however, these same knockouts also show that Hho1p is important for the transcriptional regulation of individual genes in response to stimuli, such as starvation. The differential regulation of Hho1p by phosphorylation under vegetative growth and starvation conditions has been well studied. During vegetative growth, Hho1p is phosphorylated on five closely spaced residues, preventing it from interacting with chromatin, likely by interfering with its ability to bind DNA. Under these conditions, expression is increased for CDC2, a homolog of the cyclin dependent kinases responsible for histone H1 phosphorylation, possibly creating a positive feedback loop that promotes the cell cycle. During starvation conditions, Hho1p is dephosphorylated, allowing it to bind to chromatin. This stimulates the expression of some genes, including ngoA, and protease genes such as CYP1, while inhibiting expression of other genes, such as CDC2. This decrease in CDC2 expression may be responsible for cell cycle arrest during starvation.

Associated Literature

Ref:17188762: Feng L, Miao W, Wu Y (2007) Differentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR. Aquatic toxicology (Amsterdam, Netherlands) 81(1):99-105
Ref:11972045: Dou Y, Gorovsky MA (2002) Regulation of transcription by H1 phosphorylation in Tetrahymena is position independent and requires clustered sites. Proceedings of the National Academy of Sciences of the United States of America 99(9):6142-6
Ref:11891286: Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 99(6):3734-9
Ref:12356861: Dou Y, Bowen J, Liu Y, Gorovsky MA (2002) Phosphorylation and an ATP-dependent process increase the dynamic exchange of H1 in chromatin. The Journal of cell biology 158(7):1161-70
Ref:10207086: Huang H, Smothers JF, Wiley EA, Allis CD (1999) A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila. Molecular and cellular biology 19(5):3624-34
Ref:8756729: Shen X, Gorovsky MA (1996) Linker histone H1 regulates specific gene expression but not global transcription in vivo. Cell 86(3):475-83
Ref:1996118: Dedon PC, Soults JA, Allis CD, Gorovsky MA (1991) Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes. Molecular and cellular biology 11(3):1729-33
Ref:3005971: Martindale DW, Martindale HM, Bruns PJ (1986) Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei. Nucleic acids research 14(3):1341-54
Ref:6646127: Martindale DW, Bruns PJ (1983) Cloning of abundant mRNA species present during conjugation of Tetrahymena thermophila: identification of mRNA species present exclusively during meiosis. Molecular and cellular biology 3(10):1857-65

Sequences

>TTHERM_00655890(coding)
ATGAGTGAAATAGCCATTTCAAGAAAAAGTATAAAATTAGGATTAAAGGTGCTCTTGAAT
ATTACAATTACAATATCATAATCATGTTATGACGAAATAAGCAAAGAATAAATGATTGAG
TTACTTACTATTGATTATTCTCTTCTAAAATATGGAGAGGAATTGAGAAGTATTAGAAAA
AATACACCTTAGTATGTAAATAAATATAATAGTAATAAAAATAATAATAATTATGATGAT
AAAGTATAAATTTGTATAATGGTGTGCTAGAAATCTAAGGAAGTAGCAAGCAAAGGCTAA
AATTCTGATGCTAAAAAATATAGGCATTTCCTACCAAGAAGTACTGGTTTAAACAAGCCA
TTTAATATCGTTTTAGCAAAAAAAGCAGATGAAGCAGTCTGCTTAAAATAAGCAAAGCCA
GAGAATTAGAGCGAGAGTCATAAGTTAAGAAATGGATTTAGCTGGAGCAAATAAAACAGA
TTTTACTGCCAAAAAGTAGCTTCCTATTTCATACTTCATATATTATTGAGAGCTGTACCC
TTTTTCTTAGATTAAAAGCACATGAATACTTCTGTCCATTATCAAGTGAAAGTCAGACTG
GCATACTATAATCCTAAAAGCCTAAAGGATATATATGGATATATGTAGGTAGGTAGATAG
ATAGATAGAAAGAAAGATATGTATGCATTTATGTAAGAAAGAAAATCTACTTGCTCAAAG
ATGTGTGTGTGTTTGTTTGTTCTTTTTGTTTGGTTTTTCTTTGTTTTTTCGTTTTTTTTT
TAAGAAGCAAAGAGACTGAAATCAAAAACAGGCTTGCCAAAAGTGAACTCATCCGGGAGG
GAAGGAAGACTTCTTCAGAAACCCAGAGGAAAGACAAAGGAGCTTATTTCCATTAGAATG
AATGAACAAATAGCAGCTGATTTAGAGAAGCCTTTTAGTTTTTAGTAAGAGACTTGCTAG
AAACTGAAAGTCTTGCAAAAAAGGTTTCCGAGATACCGCAGAAACACAGGAAAGAACTTT
TCAGAGCATTGGCTGATTGGTATCTGA

>TTHERM_00655890(protein)
MSEIAISRKSIKLGLKVLLNITITISQSCYDEISKEQMIELLTIDYSLLKYGEELRSIRK
NTPQYVNKYNSNKNNNNYDDKVQICIMVCQKSKEVASKGQNSDAKKYRHFLPRSTGLNKP
FNIVLAKKADEAVCLKQAKPENQSESHKLRNGFSWSKQNRFYCQKVASYFILHILLRAVP
FFLDQKHMNTSVHYQVKVRLAYYNPKSLKDIYGYMQVGRQIDRKKDMYAFMQERKSTCSK
MCVCLFVLFVWFFFVFSFFFQEAKRLKSKTGLPKVNSSGREGRLLQKPRGKTKELISIRM
NEQIAADLEKPFSFQQETCQKLKVLQKRFPRYRRNTGKNFSEHWLIGI

>TTHERM_00655890(gene)
ATGAGTGAAATAGCCATTTCAAGAAGTATGATAATAGGATATTTTATTTTTATTTGGCTT
TACTTTTATTAATATCATATTAGAAAGTATAAAATTAGGATTAAAGGTGCTCTTGAATAT
TACAATTACAATAGTACTAACTAAACTTTTAGAGATTATTATTTATTTTGGTAACTATTG
CTTACTAACTTCTCTACACACAAACAAATCTTTGTTTCTAATTTTAATTATATTTTCACT
TTTTCAACTCGATTATGAATTATTTCGTAATAGTCATAATCATGTTATGACGAAATAAGC
AAAGAATAAATGTGTGAATGATCTAAAACAATTCCCTATTAATTAAAAAAGCAAAAATCT
AAACTTTAAAAATGTGAATAATAGATTTTTTATTTTAAAAACCAAACAAATTTTTTTTTT
GTTGTAAAAATGCATTTTGATTTTTTTTTACTTAAAAATGTTTAAAATTAATTTATCATT
ATTATTATTATTAATTTATCATAAAAGGTGTATTTAAGTATTAAATATTTTTATATTTAT
GGATAAATTTTTTAATTTAATAAAAAAACATCAAAAAAAAAACTTTCTCATAAAAAATAC
TGTTTTAAACATTTCAATTTTTTTATATAAAATACATAAAAGTAAAGAATTAATGTTTTA
ATTTTTAATGATTAAAATGTTATAAATTTAGCTATTGAAAGAAATATTTGATATTTTAAT
ATTTCAAAGAGAGGCTTATTAGATAGATAGATGGTATAAAATCTATTCTTTACTAATAGC
TTATCCTATAATTTTAATCTTTATATTATTTTGAGATAAACATTTCATTTATTATGTAAT
TATAGGTAGGTAGAATATTTCATTCTTGCAAAAGCAAAAAATATTTTATTTAATTAAAAA
TGATTTCACCTAAAAAACATCATTTATTATATTAAATTTATTTTTGTAGTTCTAATCTAG
TGAAATAATTCTACTAAAAATTTTATTTTTATTTATTTATATATTTATTTTTAAAGATAG
ATAGAATAAATTTGATAAAACTTCAACTAAAATTAAATATAATAAATAAAAGCAATCCAT
TTATTTTACTTTAAACTTTAAAATATTTAAAAATTATTTTATTAAAAACTGTTATGAATT
TTTTTTTTTCATTTAATTTTATATCGAGATAATTAAGTAAACATTTTTGTTTATGAAATT
AATTTTATTCATTTAAAATTAATTGTTAAATAAGTTTGTTTCTAATTAATTATTTAAATC
TTTAAAGAAAACTCATTTCACTAAGTAAAATATTGTAAAAAGATGCTAATTTTATTATAA
TAAATTTAATTTATTTTGTTTATTTAACAAGGATTGAGTTACTTACTATTGATTATTCTC
TTCTAAAATATGGAGGTAATAATAATTAATTTAATTTTAAATAAAACCATTAAAATTTAA
TCATTTATTAAAAGAAAAACTATCTTTGAAAAGTAAATGTTTTAAGTTGGTTTTACAAGA
AACTATGATGCTAAATACAGAGATAAAGATAAAAATAAACAAAAGATTCTAAAATAATAT
TTATTTATTATACAGATCTATAAAAATAAAAAAAACAATTTCTTGTCAATTGGCAAGAGT
TTTCTCAAAGAATTTGGAAATTTTTTGCTTATTATTTACAAAAAAATCTATATATTTTTG
TTGATTGACTTTGAAAAAATATATATTATTAGATATTTTTAATATAATTTGTATAAAAAT
TATATTCTTTTTTTATGAATTTTATCAATGTTCAATAAAATTTACAAGGATTAGAATAAT
ATTTATTTTAATTGTTAAATTAATGTTATTTTAATAATTTATTGAAGGTTAATTTAATAA
TGAAATGAAGGTTTATTTTAATAGAGGAATTGAGAAGTATTAGAAAAAATACACCTTAGT
ATGTAAATAAATATAATAGTAATAAAAATAATAATAATTATGATGATAAAGTATAAATTT
GTATAATGGTGTGCTAGAAATCTAAGGAAGTAGCAAGCAAAGGCTAAAATTCTGATGCTA
AAAAATATAGGCATTTCCTACCAAGAAGTACTGGTTTAAACAAGCCATTTAATATCGTTT
TAGCAAAAAAAGCAGATGAAGCAGTCTGCTTAAAATAAGCAAAGCCAGAGAATTAGAGCG
AGGTAAATATAAGAGCAACTTATAAACATGAAACGAAATTACATTCGTAATAGAGTCATA
AGTTAAGAAATGGATTTAGCTGGAGCAAATAAAACAGATTTTACTGCCAAAAAGTAGCTT
CCTATTTCATACTTCATATATTATTGAGAGCTGTACCCTTTTTCTTAGATTAAAAGCACA
TGAATACTTCTGTCCATTATCAAGTGAAAGTCAGACTGGCATACTATAATCCTAAAAGCC
TAAAGGATATATATGGATATATGTAGGTAGGTAGATAGATAGATAGAAAGAAAGATATGT
ATGCATTTATGTAAGAAAGAAAATCTACTTGCTCAAAGATGTGTGTGTGTTTGTTTGTTC
TTTTTGTTTGGTTTTTCTTTGTTTTTTCGTTTTTTTTTTAAGAAGCAAAGAGACTGAAAT
CAAAAACAGGCTTGCCAAAAGTGAACTCATCCGGGAGGGAAGGAAGACTTCTTCAGAAAC
CCAGAGGAAAGACAAAGGAGCTTATTTCCATTAGAATGAATGAACAAATAGCAGCTGATT
TAGAGAAGCCTTTTAGTTTTTAGTAAGAGACTTGCTAGAAACTGAAAGTCTTGCAAAAAA
GGTTTCCGAGATACCGCAGAAACACAGGAAAGAAGTAAGATAATCTCTTTGTGAGCTAGA
GGTTGTTTGTTTGTTTCTTTGTTTGTTTGTTTTGGTTCTTTTTTCGTTTTTTGTTTTTTT
TTTTTAGAGAACATTTTTGAGATTCAGAAGGAGGGAAATTTGCTAAAAAACATTTTATTC
TTTGAGTGTTTTTTGTGTTTTTTGGGGTTTTTTTTGTTTCTTTTTTGTTTCTTTTTTTTT
AAAAAGCTTTTCAGAGCATTGGCTGATTGGTATCTGA

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Identifiers and Description

Gene Model Identifier

Standard Name

Aliases

Description

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Biological Process

Domains

Gene Expression Profile

Tetrahymena Stock Center

Homologs (v.2006 protein sequences)

General Information

Associated Literature

Sequences

Wiki Links

External Links

Paragraph No	Gene Name	Paragraph Text
9	HHO1, CDC2, NgoA, CYP1	HHO1 knockouts show no global increase or decrease in the amount of transcription in the cell; however, these same knockouts also show that Hho1p is important for the transcriptional regulation of individual genes in response to stimuli, such as starvation. The differential regulation of Hho1p by phosphorylation under vegetative growth and starvation conditions has been well studied. During vegetative growth, Hho1p is phosphorylated on five closely spaced residues, preventing it from interacting with chromatin, likely by interfering with its ability to bind DNA. Under these conditions, expression is increased for CDC2, a homolog of the cyclin dependent kinases responsible for histone H1 phosphorylation, possibly creating a positive feedback loop that promotes the cell cycle. During starvation conditions, Hho1p is dephosphorylated, allowing it to bind to chromatin. This stimulates the expression of some genes, including ngoA, and protease genes such as CYP1, while inhibiting expression of other genes, such as CDC2. This decrease in CDC2 expression may be responsible for cell cycle arrest during starvation.