TGD | Gene Details

Identifiers and Description

Gene Model Identifier
TTHERM_00411810

Standard Name
CDK1 (Cyclin-Dependent Kinase 1)

Aliases
PreTt21599 | 44.m00266 | 3714.m00112

Description
CDK1 cyclin-dependent kinase-like Serine/Threonine kinase family protein; Cyclin-dependent protein kinase; localizes to domains associated with mature basal bodies of ciliary rows and to both the polykineties and haplokinety of the oral apparatus; partial knockout implicates this CDK in chromatin condensation

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component
cell cortex (IDA) | GO:0005938 | Ref:12183062
Molecular Function
ATP binding (IEA) | GO:0005524
cyclin binding (ISS) | GO:0030332 | Ref:12183062
protein tyrosine kinase activity (IEA) | GO:0004713
cyclin-dependent protein serine/threonine kinase activity (ISS) | GO:0004693 | Ref:12183062
protein kinase activity (IEA) | GO:0004672
Biological Process
cortical cytoskeleton organization (IMP) | GO:0030865 | Ref:12183062
protein phosphorylation (IEA) | GO:0006468
cell cycle (IEP) | GO:0007049 | Ref:12183062
histone phosphorylation (IDA) | GO:0016572 | Ref:12183062

Domains

( PF01163 ) RIO1 family
( PF00069 ) protein kinase domain
( PF07714 ) protein tyrosine kinase

Gene Expression Profile

Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 9.9%

TetraMine Data

TTHERM_00411810

WebApollo

Fullscreen View

Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source Identifier Score Description

Tetrahymena borealis EI9_10370.1 9.997093137033517e-262 protein kinase domain-containi
ng protein (319 aa)

Oxytricha Contig5189.0.g91 1.0003068573148812e-127 Protein kinase domain

DictyBase DDB_G0288677 3.0009992673984943e-111 cdk5 on chromosome: 5 position
1885785 to 1886770

WormBase WBGene00000405 1.0003602942354155e-110 locus:cdk-1 Cyclin-dependent
kinase 1 status:Confirmed
UniProt:P34556 protein_id:C
AA81590.1

SGD YBR160W 1.9992560499818233e-109 CDC28 Catalytic subunit of the
main cell cycle cyclin-depend
ent kinase (CDK); alternately
associates with G1 cyclins (CL
Ns) and G2/M cyclins (CLBs) wh
ich direct the CDK to specific
substrates

Stentor Coeruleus SteCoe_19115 2.572209372642415e-56

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_10370.1	9.997093137033517e-262	protein kinase domain-containi ng protein (319 aa)
Oxytricha	Contig5189.0.g91	1.0003068573148812e-127	Protein kinase domain
DictyBase	DDB_G0288677	3.0009992673984943e-111	cdk5 on chromosome: 5 position 1885785 to 1886770
WormBase	WBGene00000405	1.0003602942354155e-110	locus:cdk-1 Cyclin-dependent kinase 1 status:Confirmed UniProt:P34556 protein_id:C AA81590.1
SGD	YBR160W	1.9992560499818233e-109	CDC28 Catalytic subunit of the main cell cycle cyclin-depend ent kinase (CDK); alternately associates with G1 cyclins (CL Ns) and G2/M cyclins (CLBs) wh ich direct the CDK to specific substrates
Stentor Coeruleus	SteCoe_19115	2.572209372642415e-56

General Information

Paragraph No Gene Name Paragraph Text
3 CDK1 Cyclin-dependent kinases (cdks) are a family of serine-threonine kinases that are involved in cell cycle control and cell division in eukaryotes. Cdks are catalytic subunits that are activated by association with proteins called cyclins, forming cyclin-cdk complexes. Cdk kinase activity is regulated by cyclin binding, phosphorylation and dephosphorylation, protein degradation, protein-protein interactions with cdk inhibitors, and subcellular localization.
4 CDK1 Tetrahymena thermophila Cdk1p shares homology with cdk homologs from other eukaryotes. It contains 11 catalytic domains characteristic of protein kinases, conserves all of the regulatory phosphorylation sites found in cdks, and has a slightly modified cyclin-binding PSTAIRE motif that is a hallmark of cdks. The Tetrahymena thermophila Cdk1p was also found to bind Saccharomyces cerevisiae p13suc1, a yeast cyclin.
5 CDK1 The level of Cdk1p fluctuates over the vegetative cell cycle, correlating with its histone H1 kinase activity. Cdk1p is associated with the basal bodies of the ciliary rows of the cell cortex and the oral apparatus. This localization, along with the phenotype of a partial CDK1 knockout phenotype of bent and buckled ciliary rows, suggests that Cdk1p is involved in cortical morphogenesis.

Associated Literature

Ref:16933976: Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, Collins K, Stewart BA, Lee SR, Wilamowska K, Weinberg Z, Ruzzo WL, Wloga D, Gaertig J, Frankel J, Tsao CC, Gorovsky MA, Keeling PJ, Waller RF, Patron NJ, Cherry JM, Stover NA,
Ref:12183062: Zhang H, Huang X, Tang L, Zhang QJ, Frankel J, Berger JD (2002) A cyclin-dependent protein kinase homologue associated with the basal body domains in the ciliate Tetrahymena thermophila. Biochimica et biophysica acta 1591(1-3):119-128
Ref:12045216: Doree M and Hunt T (2002) From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner? J Cell Sci 115(Pt 12):2461-4
Ref:10329641: Mizzen CA, Dou Y, Liu Y, Cook RG, Gorovsky MA, Allis CD (1999) Identification and mutation of phosphorylation sites in a linker histone. Phosphorylation of macronuclear H1 is not essential for viability in tetrahymena. The Journal of biological chemistry 274(21):14533-6
Ref:8995382: Sweet MT, Carlson G, Cook RG, Nelson D, Allis CD (1997) Phosphorylation of linker histones by a protein kinase A-like activity in mitotic nuclei. The Journal of biological chemistry 272(2):916-23
Ref:9442875: Morgan DO (1997) Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu Rev Cell Dev Biol 13:261-91
Ref:7958616: Doree M and Galas S (1994) The cyclin-dependent protein kinases and the control of cell division. FASEB J 8(14):1114-21
Ref:2065655: Roth SY, Collini MP, Draetta G, Beach D, Allis CD (1991) A cdc2-like kinase phosphorylates histone H1 in the amitotic macronucleus of Tetrahymena. The EMBO journal 10(8):2069-75

Sequences

>TTHERM_00411810(coding)
ATGAACAATAATACATTTGGCTCAGACAGATATGAAAAGCTACTTAAAGTAGGTGAAGGA
ACATATGGTGAAGTTTACAAGGCTAAAGACATTCAAAGCTCTGAAATTGTTGCTTTGAAG
AAGATTAAATTAGAAAATGAAGATGAAGGTGTCCCTTCAACTGCTCTAAGAGAAATTTCA
ATACTTAAAGAGTTACAACCACATCCCAACATCGTTTGCATGCATGAAGTTATTTATCAA
CCCTAAGAGAAGAAGTTATACCTTGTATTTGAATTTGTTGACTAAGACCTTAAAAAATTC
TTAGACTAATACCGCAAAGATAAAAAACTTTAACTTCGTCCTTACCAAATCAAACTCATG
ATGTATCAAATTTTGAATGGATTGAATTTCTGCCACAGCCGTAGAATTATTCATAGAGAT
TTAAAACCCTAAAACATTTTAATTGATGCTAAGGGAAATATTAAGATTGCTGATTTTGGT
TTGGCTAGAGCATTTGGTGTTCCTATCAAGACTCTTACTCATGAAGTTGAAACTCTTTGG
TACAGAGCTCCCGAAATTCTTTTAGGCTAAAAGGCTTATAGTCTCGGAGTTGATATTTGG
AGTTTGGGTTGCATCTTCCACGAAATGGTTGAAAAGAGAGCTCTCTTCATGGGTGACTCT
GAAATTGATTAAATATTTAAAATATTCTAATACCATGGTACTCCTACTGAGTAGACATGG
CCTGCTTTGAAGGAATGTCCTTACTTTAAACCTATATATCCTCGTTTCAAAACTGCTGAT
CCTAAGACTTACTTTAAGAATTTCTGCGATAAAGGATTTGACCTTATTTAATAGATGATT
GCTCTTGACCCTGCTAAAAGAATATCAGTAAAGGATGCTCTCAGACATCCCTACTTTGAA
GATCTTAGCAGAGAAGATATAGCTAAATTTGAGCCTAATTAAGTTCATATGTACTGA

>TTHERM_00411810(protein)
MNNNTFGSDRYEKLLKVGEGTYGEVYKAKDIQSSEIVALKKIKLENEDEGVPSTALREIS
ILKELQPHPNIVCMHEVIYQPQEKKLYLVFEFVDQDLKKFLDQYRKDKKLQLRPYQIKLM
MYQILNGLNFCHSRRIIHRDLKPQNILIDAKGNIKIADFGLARAFGVPIKTLTHEVETLW
YRAPEILLGQKAYSLGVDIWSLGCIFHEMVEKRALFMGDSEIDQIFKIFQYHGTPTEQTW
PALKECPYFKPIYPRFKTADPKTYFKNFCDKGFDLIQQMIALDPAKRISVKDALRHPYFE
DLSREDIAKFEPNQVHMY

>TTHERM_00411810(gene)
ATGAACAATAATACATTTGGCTCAGACAGATATGAAAAGCTACTTAAAGTAGGTGAAGGA
ACATATGGTGAAGTTTACAAGGCTAAAGACATTCAAAGCTCTGAAATTGTTGCTTTGAAG
AAGATTAAATTAGAAAATGAAGATGAAGGTGTCCCTTCAACTGCTCTAAGAGAAATTTCA
ATACTTAAAGAGTTACAACCACATCCCAACATCGTTTGGTAAAATTTTATTTCTCATAAC
TAAAGCGATTATTTATATTTTGTTCCTGCAATTCTCTTCAATCTTCAATTAGTACCACAC
TTTGTTTGCTACCAAATTAGTTAGTTAAAAATAATTCAAAATTTTAATGAATCCATTTTG
AAAAGATAAACTTTTGAGTTTAACAAAATTGACTGTATTTTTTATAAGATAATAGCAATT
TAGAAAAAGAGAAGAATTTATTTATTTGCCATGTTTTAATTATTTTATTTATATAATAAT
AGCATGCATGAAGTTATTTATCAACCCTAAGAGAAGAAGTTATACCTTGTATTTGAATTT
GTTGACTAAGACCTTAAAAAATTCTTAGACTAATACCGCAAAGATAAAAAACTTTAACTT
CGTCCTTACCAAATCAAAGTAAACAAATAAACTACATTCAAAAATAGTTTTTTTTTTTAA
TTTCAAAAATACCTTCTATATAATCTACCATAGAACACATGCATTCAAGCACGCAAATTA
ATTAGAAATTAGATGATCTTAATTCAATTAGCTTAAAAAAATATTATTTAAATAATTCGA
ATTCAAATAAACAGATTATCTTATTGTTTGATATTTTTATTCAATTTGAAAAAAAAGCAA
CAAAAGATTTTCAAATTAATTATCAATATATTTTGAAACATAACAATCCTTCACTCACTC
ACTCACTTTCTAACATACGCGTTAAATAAATGAATCATAGATCAATCAACTATTAAACTT
TCAATACTAAATTGGTGCAATTCTCCATTTTATTTAGTATTAAATTTAAAGTAAATGCCA
AAAATAAATAATGAGAATTTATTCTTTGATACTAAAATGAATTTATTACAAAAAAATTAG
ATAGAAATGATTTTGAAATACTTTGAAATGTTAATAAAATAAAAACTAATTTCGGTTAAA
AAATAATATATTTTATAATAATAGCTCATGATGTATCAAATTTTGAATGGATTGAATTTC
TGCCACAGCCGTAGAATTATTCATAGAGATTTAAAACCCTAAAACATTTTAATTGATGCT
AAGGGAAATATTAAGATTGCTGATTTTGGTTTGGCTAGAGCATTTGGTGTTCCTATCAAG
ACTCTTACTCATGAAGTTGAAACTCTTTGGTACAGAGCTCCCGAAATTCTTTTAGGCTAA
AAGGCTTATAGTCTCGGAGTTGATATTTGGAGTTTGGGTTGCATCTTCCACGAAATGGTT
GAAAAGAGAGCTCTCTTCATGGGTGACTCTGAAATTGATTAAATATTTAAAATATTCTAA
TACCATGGTACTCCTACTGAGTAGACATGGCCTGCTTTGAAGGAATGTCCTTACTTTAAA
CCTATATATCCTCGTTTCAAAACTGCTGATCCTAAGACTTACTTTAAGAATTTCTGCGAT
AAAGGATTTGACCTTATTTAATAGATGATTGCTCTTGACCCTGCTAAAAGAATATCAGTA
AAGGATGCTCTCAGACATCCCTACTTTGAAGATCTTAGCAGAGAAGATATAGCTAAATTT
GAGCCTAATTAAGTTCATATGTACTGA

Login

Recent Activity

Recent Papers

Identifiers and Description

Gene Model Identifier

Standard Name

Aliases

Description

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

Molecular Function

Biological Process

Domains

Gene Expression Profile

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

TetraMine Data

WebApollo

Tetrahymena Stock Center

Homologs (v.2006 protein sequences)

General Information

Associated Literature

Sequences

Wiki Links

External Links

Paragraph No	Gene Name	Paragraph Text
3	CDK1	Cyclin-dependent kinases (cdks) are a family of serine-threonine kinases that are involved in cell cycle control and cell division in eukaryotes. Cdks are catalytic subunits that are activated by association with proteins called cyclins, forming cyclin-cdk complexes. Cdk kinase activity is regulated by cyclin binding, phosphorylation and dephosphorylation, protein degradation, protein-protein interactions with cdk inhibitors, and subcellular localization.
4	CDK1	Tetrahymena thermophila Cdk1p shares homology with cdk homologs from other eukaryotes. It contains 11 catalytic domains characteristic of protein kinases, conserves all of the regulatory phosphorylation sites found in cdks, and has a slightly modified cyclin-binding PSTAIRE motif that is a hallmark of cdks. The Tetrahymena thermophila Cdk1p was also found to bind Saccharomyces cerevisiae p13suc1, a yeast cyclin.
5	CDK1	The level of Cdk1p fluctuates over the vegetative cell cycle, correlating with its histone H1 kinase activity. Cdk1p is associated with the basal bodies of the ciliary rows of the cell cortex and the oral apparatus. This localization, along with the phenotype of a partial CDK1 knockout phenotype of bent and buckled ciliary rows, suggests that Cdk1p is involved in cortical morphogenesis.