TGD | Gene Details

Identifiers and Description

Gene Model Identifier
TTHERM_00295700

Standard Name
FSF1 (Formaldehyde dehydrogenase/S-formylglutathione hydrolase fusion protein)

Aliases
PreTt15680 | 27.m00340 | 3692.m00143

Description
Formaldehyde dehydrogenase/S-formylglutathione hydrolase fusion protein; fusion protein containing two enzymes known to catalyze sequential steps in the formaldehyde detoxification pathway in yeast; this fusion unique to ciliates

Genome Browser

Gene Ontology Annotations

Molecular Function
S-formylglutathione hydrolase activity (ISS) | GO:0018738 | Ref:15858209
oxidoreductase activity (IEA) | GO:0016491
zinc ion binding (IEA) | GO:0008270
formaldehyde dehydrogenase (glutathione) activity (ISS) | GO:0004327 | Ref:15858209
Biological Process
oxidation-reduction process (IEA) | GO:0055114
formaldehyde catabolic process (ISS) | GO:0046294 | Ref:15858209

Domains

( PF00756 ) putative esterase
( TIGR01751 ) crotonyl-CoA reductase
( TIGR00692 ) L-threonine 3-dehydrogenase
( TIGR02821 ) S-formylglutathione hydrolase
( TIGR01202 ) Chlorophyll synthesis pathway, bchC
( PF08240 ) Alcohol dehydrogenase GroES-like domain
( PF00326 ) peptidase, S9A/B/C familie, catalytic domain
( PF00107 ) oxidoreductase, zinc-binding dehydrogenase family
( TIGR02822 ) zinc-binding alcohol dehydrogenase family protein
( TIGR02819 ) formaldehyde dehydrogenase, glutathione-independent
( TIGR03201 ) 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase
( TIGR03366 ) putative phosphonate catabolism associated alcohol
( TIGR02825 ) leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
( TIGR02818 ) S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Gene Expression Profile

Tetrahymena Functional Genomics Database: TTHERM_00295700

Homologs (v.2006 protein sequences)

Source Identifier Score Description

WormBase H24K24.3a 9.99796273034685e-122 WBGene00019240 alcohol dehydro
genase

FlyBase FBgn0011768 9.99870795656877e-117

TAIR AT5G43940 1.00028573512974e-115 alcohol dehydrogenase class II
I / glutathione-dependent form
aldehyde dehydrogenase / GSH-F
DH (ADHIII)

IPI(HUMAN) IPI:IPI00218446.6 1.00028573512974e-115 PROT:P11766|TREMBL:Q5U043;Q6F
I45;Q6IRT1|ENSEMBL:ENSP0000 02
96412|REFSEQ:NP_000662|H-INV:H
IT000036844;HIT000048841 Tax_I
d=9606 Alcohol dehydrogenase c
lass 3 chi chain

DictyBase DDB0204255 9.99700646136515e-115 JC3V2_0_02015 Alcohol dehydrog
enase class 3.

SGD YDL168W 1.00036029423542e-110 SFA1 SGDID:S000002327, Chr IV
from 159605-160765, Verified O
RF, "Bifunctional enzyme conta
ining both alcohol dehydrogena
se and glutathione-dependent f
ormaldehyde dehydrogenase acti
vities, functions in formaldeh
yde detoxification and formati
on of long chain and complex a
lcohols, regulated by Hog1p-Sk
o1p"

ToxoDB 583.m05453 1.00007327915485e-89 Toxoplasma gondii alcohol dehy
drogenase, putative

CryptoDB EAK88219 1.999896943452e-07 Cryptosporidium parvum (Iowa)
protein EAK88219

ParameciumDB GSPATP00000703001 0.000999755308923883 Paramecium tetraurelia GSPATP0
0000703001 protein

ParameciumDB GSPATP00012130001 0.000999755308923883 Paramecium tetraurelia GSPATP0
0012130001 protein

Source	Identifier	Score	Description
WormBase	H24K24.3a	9.99796273034685e-122	WBGene00019240 alcohol dehydro genase
FlyBase	FBgn0011768	9.99870795656877e-117
TAIR	AT5G43940	1.00028573512974e-115	alcohol dehydrogenase class II I / glutathione-dependent form aldehyde dehydrogenase / GSH-F DH (ADHIII)
IPI(HUMAN)	IPI:IPI00218446.6	1.00028573512974e-115	PROT:P11766\|TREMBL:Q5U043;Q6F I45;Q6IRT1\|ENSEMBL:ENSP0000 02 96412\|REFSEQ:NP_000662\|H-INV:H IT000036844;HIT000048841 Tax_I d=9606 Alcohol dehydrogenase c lass 3 chi chain
DictyBase	DDB0204255	9.99700646136515e-115	JC3V2_0_02015 Alcohol dehydrog enase class 3.
SGD	YDL168W	1.00036029423542e-110	SFA1 SGDID:S000002327, Chr IV from 159605-160765, Verified O RF, "Bifunctional enzyme conta ining both alcohol dehydrogena se and glutathione-dependent f ormaldehyde dehydrogenase acti vities, functions in formaldeh yde detoxification and formati on of long chain and complex a lcohols, regulated by Hog1p-Sk o1p"
ToxoDB	583.m05453	1.00007327915485e-89	Toxoplasma gondii alcohol dehy drogenase, putative
CryptoDB	EAK88219	1.999896943452e-07	Cryptosporidium parvum (Iowa) protein EAK88219
ParameciumDB	GSPATP00000703001	0.000999755308923883	Paramecium tetraurelia GSPATP0 0000703001 protein
ParameciumDB	GSPATP00012130001	0.000999755308923883	Paramecium tetraurelia GSPATP0 0012130001 protein

General Information

Paragraph No Gene Name Paragraph Text
14 FSF1 A macronuclear chromosome containing a fusion gene was cloned from the spirotrichous ciliate Oxytricha trifallax. The gene encodes a single polypeptide containing homologs of two proteins that catalyze sequential steps in the formaldehyde detoxification pathway in Saccharomyces cerevisiae. These two proteins are formaldehyde dehydrogenase (FALDH) and S-formylglutathione hydrolase (SFGH); the fusion gene is called FSF1 (FALDH/SFGH Fusion 1). A similar gene was identified in the Tetrahymena thermophila genome sequence, and a T. thermophila EST sequenced from both ends showed that the fusion gene is expressed in this species in vivo. FSF1 has not yet been identified in other ciliates, but a fusion of these two genes has been identified in another group of protists, the diatoms. An EST from Phaeodactylum tricornutum and a gene from the genome sequence of Thalassiosira pseudonana both encode a fusion of these two genes, but in the opposite orientation of the ciliate genes. In diatoms, the SFGH domain is found N-terminal to the FALDH domain, suggesting that these two fusion genes evolved independently in ciliates and diatoms. The diatom genes were named SFF1 to highlight these differences.

Associated Literature

Ref:15858209: Stover NA, Cavalcanti AR, Li AJ, Richardson BC, Landweber LF (2005) Reciprocal fusions of two genes in the formaldehyde detoxification pathway in ciliates and diatoms. Molecular biology and evolution 22(7):1539-42

Sequences

>TTHERM_00295700(coding)
ATGGACGCATCTTTACTCTCTGAATTCCTTAAAACTGCTGGCCAAACCATCACTTGTAAG
GCTGCTGTAGCTTGGGAAGCTAACAAACCTTTAGATTACACTGACATTTAAGTTGCTCCT
CCTAAGAAAGGAGAAGTTCGTATTAAAGTTTTTGCAAATGCTCTTTGCCATACTGATATT
TACACCTTAGAAGGACACGATCCTGAAGGTCTTTTCCCAAGCATTCTTGGTCATGAAGGT
ACAGGTATTGTTGAAAGTATTGGTGAAGGAGTTACTTCTGTTAAGCCTGGTGATATTGTT
ATTCCTTGCTACACACCTGAATGCAGGGAATTCTCTTGTATTTATTGTAACAATGAAAAC
ACTAACCTTTGTCCTAAGATTAGAGCCTTCTAAGGAAAGGGTTTGATGCCTGATGGTACA
AGCAGATTTTCTAAGGATGGTAAAACTATCTATCACTTTATGGGGTGTTCTAGTTTCTCT
GAATACACAGTTGTTGCTGAAATTTCTTGTGCTAAAGTTAGCGACAAGATTGATGTTAAC
CAAATGTGTCTTTTGGGTTGTGGTATCAGCACTGGTTGGGGTGCTGCAATTAATACTTGC
AAAGTAAGACCTGGATCAACAGTTGTCGTATTTGGTCTTGGTGCAGTTGGTTTAGCAGTA
GTTCAAGCTGCTAAAATTAAAGGAGCAAGAAAAATTGTTGGTATTGATCTTAACAGTGAA
AAATTCACTTTAGCTAAAGATTTCGGTTGCACTGAATGCTGGAATCCTAAAGAGTTGGAC
GGTAAGACCATTAAGGAACACCTTATTTCCTTAGAACCTTGGGGTTATGATTTCACATTT
GATTGCACTGGTATCACTGCTGTTATGAGAGATGCTTTAGAAATTGCTCACAGAGGATTT
GGTGAAAGTTGCGTTATTGGTGTTGCTGCTTCTGGTCATGAAATTTCTACTAGACCATTC
TAATTAGTTACTGGTAGATAATGGAAAGGAACTGCCTTTGGTGGATGGAAAAGCAGAACT
GAAGTTCCCTAATTAGTTTAACAAGTATCTACTGGGGAATTGCCAATTCAAAAATACATT
ACTCATGAAATTAAGGGCTTACAAAATGTCAACTAAGCTATTGATGCTTTACACTCAGGA
AAATGCTTGCGTGCAGTTGTTCATATCAACGAAAGACCTGATTTCAACAAAGAATAGGTA
TCAAAGTTAGTCCCTCGTGTTTATTCTAATGCAAGAAGTCATAATGGATGGGTAAAGAGA
GTAAAGCATTATTCTGAATCTCTTTAATGCGAAATGCAATTTTCAATTTACATCCCTGAT
AAGAAGTCTCGTAATGCTTAACTTCCCCCTGTTCTTTATTATCTTAGTGGACTTACTTGC
ACTGATGAAAACGCTTTGGTAAAATCTGGCTTCTAAAGATATGCCTCTGAACTCGGTATT
GCTGTTGTATTCCCTGACACCTCACCTCGTGTTGATATTGAAGGTGATAACGAAAGTTGG
GACTTTGGTAAATCAGCTGGATTCTACTTAAATGCTACTTAAGAAAAATGGAGAAAGAAC
TACAATATGTTCACCTATATCTCTGAAGAATTACCAAAATTGATTGGAGAATTCTTCCCT
GTTGATGTAAGCAGAGCTGGTATTACTGGTCACTCTATGGGTGGTATGGGAGCTCTTAAC
ATATTCTTAAAGAACCCAGGTAAATATTTGAGTGTCTCTGCCTTTGCTCCTATTTGCAAT
CCCACTTAGTGCCCTTGGGGAGTTAAGGCTTTCACTAACTATTTAGGCTCTGTAGAAGCT
GGTAAATAGTATGATCCAACTGAACTTATTGTTTAATATAATGGTCCCAAGGCTAAGATT
TTAATTGATCAAGGTACTCATGACTCATTCCTTTACAATTAACTTCATCCTTAAAATTTC
TTAAAGGCTGCATCTTCAGTTAATTACCCTGTTGAATTCAGATATCAAAATAACTATGAT
CACCTCTACTTCTTTGTAGAGACCTTCATGGGAGATCATTTCAAGCACCATGCTTAATAT
CTTCTTAGATGA

>TTHERM_00295700(protein)
MDASLLSEFLKTAGQTITCKAAVAWEANKPLDYTDIQVAPPKKGEVRIKVFANALCHTDI
YTLEGHDPEGLFPSILGHEGTGIVESIGEGVTSVKPGDIVIPCYTPECREFSCIYCNNEN
TNLCPKIRAFQGKGLMPDGTSRFSKDGKTIYHFMGCSSFSEYTVVAEISCAKVSDKIDVN
QMCLLGCGISTGWGAAINTCKVRPGSTVVVFGLGAVGLAVVQAAKIKGARKIVGIDLNSE
KFTLAKDFGCTECWNPKELDGKTIKEHLISLEPWGYDFTFDCTGITAVMRDALEIAHRGF
GESCVIGVAASGHEISTRPFQLVTGRQWKGTAFGGWKSRTEVPQLVQQVSTGELPIQKYI
THEIKGLQNVNQAIDALHSGKCLRAVVHINERPDFNKEQVSKLVPRVYSNARSHNGWVKR
VKHYSESLQCEMQFSIYIPDKKSRNAQLPPVLYYLSGLTCTDENALVKSGFQRYASELGI
AVVFPDTSPRVDIEGDNESWDFGKSAGFYLNATQEKWRKNYNMFTYISEELPKLIGEFFP
VDVSRAGITGHSMGGMGALNIFLKNPGKYLSVSAFAPICNPTQCPWGVKAFTNYLGSVEA
GKQYDPTELIVQYNGPKAKILIDQGTHDSFLYNQLHPQNFLKAASSVNYPVEFRYQNNYD
HLYFFVETFMGDHFKHHAQYLLR

>TTHERM_00295700(gene)
TAAATATTGAATCAATTTATAAATTGTCTAAAAAATTTTAAGTTTTTCAAATGGACGCAT
CTTTACTCTCTGAATTCCTTAAAACTGCTGGCCAAACCATCACTTGTAAGGCTGCTGTAG
CTTGGGAAGCTAACAAACCTTTAGATTACACTGACATTTAAGTTGCTCCTCCTAAGAAAG
GAGAAGTTCGTATTAAAGTTTTTGCAAATGCTCTTTGCCATACTGATATTTACACCTTAG
AAGGACACGATCCTGAAGGTCTTTTCCCAAGCATTCTTGGTCATGAAGGTACAGGTATTG
TTGAAAGTATTGGTGAAGGAGTTACTTCTGTTAAGCCTGGTGATATTGTTATTCCTTGCT
ACACACCTGAATGCAGGGAATTCTCTTGTATTTATTGTAACAATGAAAACACTAACCTTT
GTCCTAAGATTAGAGCCTTCTAAGGTAAAAATTACTTTTATTTATTAATAATTAATATAG
CTTAGCTTTCTATGAAGAACTACAACAATACTATGACTTTTAAGGAATTTTATTAAAAGG
ATAAAGGCATAATTTATAAATTCTACTTACCATTAAATGATAAAGTGTAATATTATTAAT
TTTAATTAAAGGAAAGGGTTTGATGCCTGATGGTACAAGCAGATTTTCTAAGGATGGTAA
AACTATCTATCACTTTATGGGGTGTTCTAGTTTCTCTGAATACACAGTTGTTGCTGAAAT
TTCTTGTGCTAAAGTTAGCGACAAGATTGATGTTAACCAAATGTGTCTTTTGGGTTGTGG
TATCAGCACTGGTTGGGGTGCTGCAATTAATACTTGCAAAGTAAGACCTGGATCAACAGT
TGTCGTATTTGGTCTTGGTGCAGTTGGTTTAGCAGTAGTTCAAGCTGCTAAAATTAAAGG
AGCAAGAAAAATTGTTGGTATTGATCTTAACAGTGAAAAATTCACTTTAGCTAAAGATTT
CGGTTGCACTGAATGCTGGAATCCTAAAGAGTTGGACGGTAAGACCATTAAGGAACACCT
TATTTCCTTAGAACCTTGGGGTTATGATTTCACATTTGATTGCACTGGTATCACTGCTGT
TATGAGAGATGCTTTAGAAATTGCTCACAGAGGGTAATTAGTTTTTAAAAGATGTTTTAT
ATAGATATTCAAAATAGTTAATCAGTAACAAAAAAGATTCTTTGTGAATTGGTTAGGAAT
AGGAAGAGAAAATTCACAATTTAATAAAAAAAATTTTATTGATAATTAATTTTTTAGGGT
TTCCATCTAATTTTGAATATTTTTAATTACTATTGATCTTTGAATAAACAAGCAAATGTA
GGATTAAATTTATTAAATAAATTTTATGATTATTAATTATATAGATTTGGTGAAAGTTGC
GTTATTGGTGTTGCTGCTTCTGGTCATGAAATTTCTACTAGACCATTCTAATTAGTTACT
GGTAGATAATGGAAAGGAACTGCCTTTGGTGGATGGAAAAGCAGAACTGAAGTTCCCTAA
TTAGTTTAACAAGTATCTACTGGGGAATTGCCAATTCAAAAATACATTACTCATGAAATT
AAGGGCTTACAAAATGTCAACTAAGCTATTGATGCTTTACACTCAGGAAAATGCTTGCGT
GCAGTTGTTCATATCAACGAAAGACCTGATTTCAACAAAGAATAGGTATCAAAGTTAGTC
CCTCGTGTTTATTCTAATGCAAGAAGTCATAATGGATGGGTAAAGAGAGTAAAGCATTAT
TCTGAATCTCTTTAATGCGAAATGCAATTTTCAATTTACATCCCTGATAAGAAGTCTCGT
AATGCTTAACTTCCCCCTGTTCTTTATTATCTTAGTGGACTTACTTGCACTGATGAAAAC
GCTTTGGTAAAATCTGGCTTCTAAAGATATGCCTCTGAACTCGGTATTGCTGTTGTATTC
CCTGACACCTCACCTCGTGTTGATATTGAAGGTGATAACGAAAGTTGGGACTTTGGTAAA
TCAGCTGGATTCTACTTAAATGCTACTTAAGAAAAATGGAGAAAGAACTACAATATGTTC
ACCTATATCTCTGAAGAATTACCAAAATTGATTGGAGAATTCTTCCCTGTTGATGTAAGC
AGAGCTGGTATTACTGGTCACTCTATGGGTGGTATGGGAGCTCTTAACATATTCTTAAAG
AACCCAGGTAAATATTTGAGTGTCTCTGCCTTTGCTCCTATTTGCAATCCCACTTAGTGC
CCTTGGGGAGTTAAGGCTTTCACTAACTATTTAGGCTCTGTAGAAGCTGGTAAATAGTAT
GATCCAACTGAACTTATTGTTTAATATAATGGTCCCAAGGCTAAGATTTTAATTGATCAA
GGTACTCATGACTCATTCCTTTACAATTAACTTCATCCTTAAAATTTCTTAAAGGCTGCA
TCTTCAGTTAATTACCCTGTTGAATTCAGATATCAAAATAACTATGATCACCTCTACTTC
TTTGTAGAGACCTTCATGGGAGATCATTTCAAGCACCATGCTTAATATCTTCTTAGATGA

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Identifiers and Description

Gene Model Identifier

Standard Name

Aliases

Description

Genome Browser

Gene Ontology Annotations

Molecular Function

Biological Process

Domains

Gene Expression Profile

Homologs (v.2006 protein sequences)

General Information

Associated Literature

Sequences

Wiki Links

External Links

Paragraph No	Gene Name	Paragraph Text
14	FSF1	A macronuclear chromosome containing a fusion gene was cloned from the spirotrichous ciliate Oxytricha trifallax. The gene encodes a single polypeptide containing homologs of two proteins that catalyze sequential steps in the formaldehyde detoxification pathway in Saccharomyces cerevisiae. These two proteins are formaldehyde dehydrogenase (FALDH) and S-formylglutathione hydrolase (SFGH); the fusion gene is called FSF1 (FALDH/SFGH Fusion 1). A similar gene was identified in the Tetrahymena thermophila genome sequence, and a T. thermophila EST sequenced from both ends showed that the fusion gene is expressed in this species in vivo. FSF1 has not yet been identified in other ciliates, but a fusion of these two genes has been identified in another group of protists, the diatoms. An EST from Phaeodactylum tricornutum and a gene from the genome sequence of Thalassiosira pseudonana both encode a fusion of these two genes, but in the opposite orientation of the ciliate genes. In diatoms, the SFGH domain is found N-terminal to the FALDH domain, suggesting that these two fusion genes evolved independently in ciliates and diatoms. The diatom genes were named SFF1 to highlight these differences.