Identifiers and Description

Gene Model Identifier

TTHERM_01107220

Standard Name

TPB2 (Tetrahymena PiggyBac transposase 2)

Aliases

PreTt28071 | 237.m00028 | 3794.m00164

Description

PiggyBac transposase, tpb2p; produces double-strand breaks with four-base 5' protruding ends on DNA; involved in DNA cleavage step of programmed DNA deletion

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• heterochromatin (IDA) | GO:0000792 | Ref:20357003
• macronucleus (IDA) | GO:0031039 | Ref:20357003

Molecular Function

• endodeoxyribonuclease activity (IDA) | GO:0004520 | Ref:20357003

Biological Process

• heterochromatin assembly (IMP) | GO:0031507 | Ref:20357003
• programmed DNA elimination (IMP) | GO:0031049 | Ref:20357003

Domains

No Data fetched for Domains

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10%

TetraMine Data

TTHERM_01107220

WebApollo

Fullscreen View

Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_20298.1	4.0002634815779907e-110	hypothetical protein (509 aa)

General Information

No Data fetched for General Information

Associated Literature

1. Ref:24348275: Vogt A, Mochizuki K (2013) A domesticated PiggyBac transposase interacts with heterochromatin and catalyzes reproducible DNA elimination in Tetrahymena. PLoS genetics 9(12):e1004032
2. Ref:23935529: Vogt A, Goldman AD, Mochizuki K, Landweber LF (2013) Transposon domestication versus mutualism in ciliate genome rearrangements. PLoS genetics 9(8):e1003659
3. Ref:20357003: Cheng CY, Vogt A, Mochizuki K, Yao MC (2010) A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila. Molecular biology of the cell 21(10):1753-62

Sequences

>TTHERM_01107220(coding)
ATGAAAAGAGATATTAACACGCCTATTGATAAAATGAAATTTTACTAGCCTTTAGATAAA
GAAGGCTAGCCTGTTGTAATGTATGACGATGGTACTATAAAAAAATTAGGACAAAAAAGA
ACTTATGAAGGTTAACCTGAATAGTATATCGTTAAAACAAAAAAGAACGATGTTTACTTA
GTTGAAAAATCAAAGAAAGTTAGAAAAGCTAGAAAAAGAAAATAATTTTAAGAAGGAGAA
GATGCTAAGAAACCTTTAACAGAAAAAGAAGTGGAAAAGAGATGGACTGAATTCTCTAAA
GAAAATAAAATGAGACAATAACATAAAGAAGATGATTTGATTGATGATCAATGGAGAAGA
ATATATTCTGTAGATTAAGTTCCAAAAAGCTTTTATATGAAAACTAAATTTGATAAAGAT
TTGAATAGAGATTTTATTCCATATAATGTACCTCATTCCCCATATCATCTGTTCAGAATG
TTTTTTGACGATAGAATATATAAATTGATTATTGAAGAAACTAACCGATATAAACATCAG
AAATATTAAGAGGCCTTACTAAATCTACCTCCTGATAAAACTCTTCCCAAAGAGGCTATG
GACATAGATTCTATAGATTTAGATGCATATCTTTCAATTTTGATATTTATGGGTGTGTAA
AGAATGAAAAGTGTTAAAGACTATTGGAAGAGGAAAAATTATATTAATAGTGAAATAGCG
TGCATAATGAAGTATTCTAGATTTGAATAAATAGACAAACATTTACATTTAGCAGATAAT
GAAGACCCTAAAATCAGAAGTGATCCTATTGGAAAAGTTAGATAATATATGGATTATTTA
AATGAAAATTTTAAGAAATACTATTACCCAGGAGAGTTCCTAGCTATTGATGAAGGTATG
ATACCTTTTAATGGCAAAGTCGCTTTCAAGGTCTATAATCCTGATAAACCCGATAAGTTT
GGTATCAAAGAATATGTTTGTTGTGATTCTTAAAACGCTTACACTTTAGAATCTTAGCTC
TATTATGGTAACCATGAAAATGAAGAATTCCCAGAAATTACTCTTTCCAAAACTAATGAA
GTTGTAATGAATTTACTAAAAGACTATGAAGGCAGATTTCATAAAGTAGTAATGGATAAT
TATTATAACAGTCCAACTTTGTTTTATCTGATGAAATAAAAATCTTTTGGTGCTCTAGGT
ACCATGAGAATCGGGCGTCTAAAATTACCTCCTTATTTACTCTCTTAAATTATGGAAGTC
CATCAAAATAAGGTTTTATCTTACATTTAAGGTGATATCAATCTGGCTATTTTCTTCAGA
GGAACAAACGATAAAGAAATTTGCCTAATGTCAAATTTCATAGATTAAAAAAAGGTTAAA
GATGATTTTTGGAGAGTTCTTGCGTACGTTAAAGAAGACAGAGATATTTTCAAAGAGTAA
AATTTATAGAGCGCTTATTATAACAAATACAAAGGAGGTGTTGATAGAAGAAACAGTTAT
CTCGCAGCATATAGAAATTGTAGGAAGAATATTAAATGGTATAGGCCCGTCTTTTATAGA
ATGTTAGATAATGCGATAGTTAATGCATTTATCCTTTATAATTTTAATTTAAGTCCTAAA
TATAAAATTTCACAAAAAGAATTTAGAATATAGCTATTTAAGGAACTTGCTTGCAGATAT
TCTCCATCTAAAAGCCAACACGCAATCAAAGTCCAATAATTTATGAAAGATAAATCTGCT
GAAGTGGATAAAATACCACAATATTTTGAAAAAGCTATGAAACTTCATATTATGAAAAAT
TGTGATTACTCAGCTCATATTCTTGTTAGATCCCGTACTAAAAAAAAATCTTGCATTGAA
TGCAAATAATTAACTTTATTTTCTTGCAGTACATGCTCAAATATGTTTAAGATGAGAATA
CCTCTTTGTTAATCAGGTTTCAACTAATGCTATGATTTCCATGCAAGTAAAACATATGAA
GAGGTTGTTGAAGATATGTTTACTAAGAGAAGAAAAGATGGAAGAAAATTAGATATCGAA
ATTAAAAAGAAAAAGAAAAAATTAGTTAAGCTCTCAGATGTTTATGATTCAATATTAAAG
CCAAAATAAAGCAATTAAAATCCAATACAAGTAAAAACTAAACAAATCCTATAAAAATCT
GTTACTATTGATCACTACACAAGCAATAAAGCATTTTTAGAAGATGATATGTATTCAGAA
AACTAACCTAATTCAACTGAATTAAATGATCAGTCTACTCGTATGAAGACAAACTAAATC
ATGAGCTCTTCTAATTCAAATTCAATATAAGGAAGTAAAAAAAAAAAGTTCAAGGTCATT
AAAAAGGAAAAAAATTTAAAAACTCAAAAGACAAAATCTTGTGATGCAATATTTAGCAGT
GAGAAGATAAAAAAGAAATATACTGATGAAATAAATGAATTTTTTGATTCAAAAGCTTCC
ATTAGGTTAACTAATTTCTTTTAAGAGAGCTAACGTCTTTAAAATTTACTGAAAGAATCA
TAAGAAAATCTTTCTAAAAAATTTGTTGTTTTAGATTAGACCAGTGATGAAGACTTTAAA
AATAATAACACAGACGAAGATCTCTAATGGATAAATACAACTTTAGAGAAAATAAATTAA
AAAAAAATAGAACCTAACGAAAAATTATCTCCTGGCCTTGGTATAATAAACAAAAAATAA
GAGGCAGAAGAAGTAATAGAAGTATTATCAAGCAGCTCTACTCTAAACGAAGAAAAAGTA
AGAACTAAATATTAAGAAATTCCTTTTACTTTTGGAAGCATTCTTGAAGTAGAACAAGAA
AAAGAAGAATCAGATGATGAAGTTGCGAAGGAATACAAAAAAGTTTCTAAAAGAATAGAA
GAATTTAATGAAGATTCCATTCATTCAAAATAACAACTAGAGGACAGCAATTTTTTTCCA
TAAAAAGCTGGAGTGATTTTTTACTCGATGAGTGAATTAGATAAAAAAAAAGAATAAGTA
ATAAGTGACTAAAATAAAGAATAGAATTAAATTATAGTTAAGTAAGAACATAGATCTGAC
TAGAAAAAAAAGAATAGCTATCCTCTATAACTTTATGACTGTGAAAATGTCCTGAACAAT
GCCATAGATTTTTCTGATTACTCATTTTCATCTTCTGATGAAGAACAATTTGTTTATAAT
AAAAATAAGAACAAATAACAAGAATTAAATCACGAATCAGATACAAATAATCCCTAACTA
TAATACAAAGGCAAAATAAAAAATAATAAATTTAAAATTGATGAGAGCTTTGATGAAAGC
AATCTTAAAATAGAAAGGCCTTTTGATTTAATTCCATTTACCTTTTAAAATGATGCAATG
TAAACTAGTTAAAATTTTGTAGTCATTGATGATGACAGTGATGTAGAATTTTAAAATATA
AATTCTTAGATAATAAATTAATCAAGCATAATTTCAGAAAGTTAAAAATTGGAATTTGAA
GGATTTATTGATAATCCATAACCAACTAAAAAAAGGATAATCTCTTGCCAAGACATGAAA
AGAAAAATTATTATTAAAAAAAATAAAGAGCCAAAAAATGATAATTAGAATATCATTTAC
ATTAACCATTAACGTTTTAAAATAGTGAAGCTAAAAACTACTATATAAATTGACTCAGAC
TGA

>TTHERM_01107220(protein)
MKRDINTPIDKMKFYQPLDKEGQPVVMYDDGTIKKLGQKRTYEGQPEQYIVKTKKNDVYL
VEKSKKVRKARKRKQFQEGEDAKKPLTEKEVEKRWTEFSKENKMRQQHKEDDLIDDQWRR
IYSVDQVPKSFYMKTKFDKDLNRDFIPYNVPHSPYHLFRMFFDDRIYKLIIEETNRYKHQ
KYQEALLNLPPDKTLPKEAMDIDSIDLDAYLSILIFMGVQRMKSVKDYWKRKNYINSEIA
CIMKYSRFEQIDKHLHLADNEDPKIRSDPIGKVRQYMDYLNENFKKYYYPGEFLAIDEGM
IPFNGKVAFKVYNPDKPDKFGIKEYVCCDSQNAYTLESQLYYGNHENEEFPEITLSKTNE
VVMNLLKDYEGRFHKVVMDNYYNSPTLFYLMKQKSFGALGTMRIGRLKLPPYLLSQIMEV
HQNKVLSYIQGDINLAIFFRGTNDKEICLMSNFIDQKKVKDDFWRVLAYVKEDRDIFKEQ
NLQSAYYNKYKGGVDRRNSYLAAYRNCRKNIKWYRPVFYRMLDNAIVNAFILYNFNLSPK
YKISQKEFRIQLFKELACRYSPSKSQHAIKVQQFMKDKSAEVDKIPQYFEKAMKLHIMKN
CDYSAHILVRSRTKKKSCIECKQLTLFSCSTCSNMFKMRIPLCQSGFNQCYDFHASKTYE
EVVEDMFTKRRKDGRKLDIEIKKKKKKLVKLSDVYDSILKPKQSNQNPIQVKTKQILQKS
VTIDHYTSNKAFLEDDMYSENQPNSTELNDQSTRMKTNQIMSSSNSNSIQGSKKKKFKVI
KKEKNLKTQKTKSCDAIFSSEKIKKKYTDEINEFFDSKASIRLTNFFQESQRLQNLLKES
QENLSKKFVVLDQTSDEDFKNNNTDEDLQWINTTLEKINQKKIEPNEKLSPGLGIINKKQ
EAEEVIEVLSSSSTLNEEKVRTKYQEIPFTFGSILEVEQEKEESDDEVAKEYKKVSKRIE
EFNEDSIHSKQQLEDSNFFPQKAGVIFYSMSELDKKKEQVISDQNKEQNQIIVKQEHRSD
QKKKNSYPLQLYDCENVLNNAIDFSDYSFSSSDEEQFVYNKNKNKQQELNHESDTNNPQL
QYKGKIKNNKFKIDESFDESNLKIERPFDLIPFTFQNDAMQTSQNFVVIDDDSDVEFQNI
NSQIINQSSIISESQKLEFEGFIDNPQPTKKRIISCQDMKRKIIIKKNKEPKNDNQNIIY
INHQRFKIVKLKTTIQIDSD

>TTHERM_01107220(gene)
ATGAAAAGAGATATTAACACGCCTATTGATAAAATGAAATTTTACTAGCCTTTAGATAAA
GAAGGCTAGCCTGTTGTAATGTATGACGATGGTACTATAAAAAAATTAGGACAAAAAAGA
ACTTATGAAGGTAATTCTTAATTTTTAAAAATTAAATTTACTCATATTTATTAAAATATA
GGTTAACCTGAATAGTATATCGTTAAAACAAAAAAGAACGATGTTTACTTAGTTGAAAAA
TCAAAGAAAGTTAGAAAAGCTAGAAAAAGAAAATAATTTTAAGAAGGAGAAGATGCTAAG
AAACCTTTAACAGGTAAAATTTTTTTAGTATGGCAATTTAAATTAAAGTGTACCATTAAC
TATAGAAAAAGAAGTGGAAAAGAGATGGACTGAATTCTCTAAAGAAAATAAAGTAAAAAT
AATTATTAGTAAATTTTAAAGTAAAGCTATTTTATAAATACAGATGAGACAATAACATAA
AGAAGATGATTTGATTGATGATCAATGGAGAAGAATATATTCTGTAGATTAAGTTCCAAA
AAGCTTTTATATGAAAACTAAATTTGATAAAGATTTGAATAGAGTAAACGTTTTTTTTCT
CATTTTAAATTAAAATTTAACTTGACATTTAAATAAATTAGGATTTTATTCCATATAATG
TACCTCATTCCCCATATCATCTGTTCAGAATGTTTTTTGACGATAGAATATATAAATTGA
TTATTGAAGAAACTAACCGATATAAACATCAGAAATATTAAGAGGCCTTACTAAATCTAC
CTCCTGATAAAACTCTTCCCAAAGAGGCTATGGACATAGATTCTATAGATTTAGATGCAT
ATCTTTCAATTTTGATATTTATGGGTGTGTAAAGAATGAAAAGTGTTAAAGGTAAATAAT
TTTTTTATTACTTATTTATGACCTTTCAAAAATTTAATTAGAGACTTTTTAAAAATATTG
TCCATGCTTTTCTGAATAAAAATTTTAATGCAAAATTAGACTATTGGAAGAGGAAAAATT
ATATTAATAGTGAAATAGCGTGCATAATGAAGTATTCTAGATTTGAATAAATAGACAAAC
ATTTACATTTAGCAGATAATGAAGACCCTAAAATCAGAAGTGATCCTATTGGAAAAGTTA
GATAATATATGGATTATTTAAATGAAAATTTTAAGAAATACTATTACCCAGGAGAGTTCC
TAGCTATTGATGAAGGTATGATACCTTTTAATGGCAAAGTCGCTTTCAAGGTCTATAATC
CTGATAAACCCGATAAGGTATACTTTTATCATTTTTATTTATTGTTTTAAAAAATTTTTT
CTAGTTTGGTATCAAAGAATATGTTTGTTGTGATTCTTAAAACGCTTACACTTTAGAATC
TTAGCTCTATTATGGTAACCATGAAAATGAAGAATTCCCAGAAATTACTCTTTCCAAAAC
TAATGAAGGTTTTTTTAGTTTTTTCGAAATCAGTTTTGAAATTAATATTTATTATTAAAA
GTTGTAATGAATTTACTAAAAGACTATGAAGGCAGATTTCATAAAGTAGTAATGGATAAT
TATTATAACAGTCCAACTTTGTTTTATCTGATGAAATAAAAATCTTTTGGTGCTCTAGGT
ACCATGAGAATCGGGCGTCTAAAATTACCTCCTTATTTACTCTCTTAAATTATGGAAGTC
CATCAAAATAAGGTTTTAAATTATTTCTAAGTTGTGTATAAAATAACAAACTTAAAAAAA
TATATAAAAGGTTTTATCTTACATTTAAGGTGATATCAATCTGGCTATTTTCTTCAGAGG
AACAAACGATAAAGAAATTTGCCTAATGTCAAATTTCATAGATTAAAAAAAGGTTAAAGA
TGATGTAAATGTTTTCAAAATTTTCTTTCTCTTACTCATTGATTTTAAAAATAGTTTTGG
AGAGTTCTTGCGTACGTTAAAGAAGACAGAGATATTTTCAAAGAGTAAAATTTATAGGTA
AAAAAATTTTATTAAATGATAATTTTTATTTATTTTAAATCTTATTTTGATTAAATTTAT
CATGAAAAATGCAAATACAACCAAATAAGCTAATAGCTTAAGTTATAAGAAATAAAAATA
GCAATATTATTTTTAAATACCTAAATTTAGAGCGCTTATTATAACAAATACAAAGGAGGT
GTTGATAGAAGAAACAGTTATCTCGCAGCATATAGAAATTGTAGGAAGAATATTAAATGG
TATAGGCCCGTCTTTTATAGAATGTTAGATAATGCGATAGTTAATGCATTTATCCTTTAT
AATTTTAATTTAAGTCCTAAATATAAAATTTCACAAAAAGAATTTAGAATATAGCTATTT
AAGGAACTTGCTTGCAGGTAAAATTTTAATTATAAGAATTTTTTATTAATTTTATATAAA
AACATTAGATATTCTCCATCTAAAAGCCAACACGCAATCAAAGTCCAATAATTTATGAAA
GATAAATCTGCTGAAGTGGATAAAATACCACAATATTTTGAAAAAGCTATGAAACTTCAT
ATTATGAAAAATTGTGATTACTCAGCTCATATTCTTGTTAGATCCCGTACTAAAAAAAAA
TCTTGCATTGAATGCAAATAATTAACTTTATTTTCTTGCAGTACATGCTCAAATATGTTT
AAGATGAGAATACCTCTTTGTTAATCAGGTTTCAACTAAGTAAATTAATAAATATTTAAT
AGTAAATTAAATTACATTCATTAAATTACAGTGCTATGATTTCCATGCAAGTAAAACATA
TGAAGAGGTTGTTGAAGATATGTTTACTAAGAGAAGAAAAGATGGAAGAAAATTAGATAT
CGAAATTAAAAAGAAAAAGAAAAAATTAGTTAAGCTCTCAGATGTTTATGATTCAATATT
AAAGCCAAAATAAAGCAATTAAAATCCAATACAAGTAAAAACTAAACAAATCCTATAAAA
ATCTGTTACTATTGATCACTACACAAGCAATAAAGCATTTTTAGAAGATGATATGTATTC
AGAAAACTAACCTAATTCAACTGAATTAAATGATCAGTCTACTCGTATGAAGACAAACTA
AATCATGAGCTCTTCTAATTCAAATTCAATATAAGGTTATATTTTTCGAAAATTTTTATT
GAAATTTCATAAAATGTTTTATGAACAGGAAGTAAAAAAAAAAAGTTCAAGGTCATTAAA
AAGGAAAAAAATTTAAAAACTCAAAAGACAAAATCTTGTGATGCAATATTTAGCAGTGAG
AAGATAAAAAAGAAATATACTGATGAAATAAATGAATTTTTTGATTCAAAAGCTTCCATT
AGGTTAACTAATTTCTTTTAAGAGAGCTAACGTCTTTAAAATTTACTGAAAGAATCATAA
GAAAATCTTTCTAAAAAATTTGTTGTTTTAGATTAGACCAGTGATGAAGACTTTAAAAAT
AATAACACAGACGAAGATCTCTAATGGATAAATACAACTTTAGAGAAAATAAATTAAAAA
AAAATAGAACCTAACGAAAAATTATCTCCTGGCCTTGGTATAATAAACAAAAAATAAGAG
GCAGAAGAAGTAATAGAAGTATTATCAAGCAGCTCTACTCTAAACGAAGAAAAAGTAAGA
ACTAAATATTAAGAAATTCCTTTTACTTTTGGAAGCATTCTTGAAGTAGAACAAGAAAAA
GAAGAATCAGATGATGAAGTTGCGAAGGAATACAAAAAAGTTTCTAAAAGAATAGAAGAA
TTTAATGAAGATTCCATTCATTCAAAATAACAACTAGAGGACAGCAATTTTTTTCCATAA
AAAGCTGGAGTGATTTTTTACTCGATGAGTGAATTAGATAAAAAAAAAGAATAAGTAATA
AGTGACTAAAATAAAGAATAGAATTAAATTATAGTTAAGTAAGAACATAGATCTGACTAG
AAAAAAAAGAATAGCTATCCTCTATAACTTTATGACTGTGAAAATGTCCTGAACAATGCC
ATAGATTTTTCTGATTACTCATTTTCATCTTCTGATGAAGAACAATTTGTTTATAATAAA
AATAAGGTAAAATACTTTTAAATAAATTGTCAAATAAATATTTTTAATAAAATTTTATAG
AACAAATAACAAGAATTAAATCACGAATCAGATACAAATAATCCCTAACTATAATACAAA
GGCAAAATAAAAAATAATAAATTTAAAATTGATGAGAGCTTTGATGAAAGCAATCTTAAA
ATAGAAAGGCCTTTTGATTTAATTCCATTTACCTTTTAAAATGATGCAATGTAAACTAGT
TAAAATTTTGTAGTCATTGATGATGACAGTGATGTAGAATTTTAAAATATAAATTCTTAG
ATAATAAATTAATCAAGCATAATTTCAGAAAGTTAAAAATTGGAATTTGAAGGATTTATT
GATAATCCATAACCAACTAAAAAAAGGATAATCTCTTGCCAAGACATGAAAAGAAAAATT
ATTATTAAAAAAAATAAAGAGCCAAAAAATGATAATTAGAATATCATTTACATTAACCAT
TAACGTTTTAAAATAGTGAAGCTAAAAACTACTATATAAATTGACTCAGACTGA