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Identifiers and Description
Gene Model Identifier
THD1 (Tetrahymena Histone Deacetylase )
PreTt15839 | 93.m00135 | 3727.m00055
Histone deacetylase; homolog of yeast Rpd3p; localized to the macronucleus during vegetative growth; deacetylates all four core histones in vitro; knockdown cells exhibit extrusion bodies and reduced phosphorylation of histone H1
THD1, a homolog of the Saccharomyces cerevisiae Rpd3p, a class I histone deacetylase (HDAC), is localized to the macronucleus during vegetative growth, and distributed to developing new macronuclei early in their differentiation. Thd1p deacetylates all four core histones in vitro. Thd1p is a 52kDa polypeptide in an HDAC complex of approximately 160 kDa.
Tetrahymena cells with reduced Thd1p expression exhibited phenotypes indicative of loss of chromatin integrity, such as DNA fragmentation and extrusion of chromatin from the macronucleus, variable macronuclear size and shape, enlarged nucleoli, and reduced phosphorylation of histone H1 from bulk chromatin. Macronuclei in THD1 knockdown cells also contained more DNA, suggesting Thd1p may play a role in regulating macronuclear DNA content. The THD1 gene could not be completely replaced by a disruption construct, suggesting that THD1 is an essential gene.
Ref:18178773: Smith JJ, Torigoe SE, Maxson J, Fish LC, Wiley EA (2008) A class II histone deacetylase acts on newly synthesized histones in Tetrahymena. Eukaryotic cell 7(3):471-82
Ref:17715364: Parker K, Maxson J, Mooney A, Wiley EA (2007) Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila. Eukaryotic cell 6(10):1913-24
Ref:15879532: Wiley EA, Myers T, Parker K, Braun T, Yao MC (2005) Class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila. Eukaryotic cell 4(5):981-90
Ref:11046129: Wiley EA, Ohba R, Yao MC, Allis CD (2000) Developmentally regulated rpd3p homolog specific to the transcriptionally active macronucleus of vegetative Tetrahymena thermophila. Molecular and cellular biology 20(22):8319-28
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