Identifiers and Description

Gene Model Identifier

TTHERM_00193849

Standard Name

RPB3 (RNA Polymerase II subunit 3)

Aliases

16.m05348 | cnjC

Description

DNA-directed rna polymerase II subunit

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Molecular Function

• DNA-directed 5'-3' RNA polymerase activity (IEA) | GO:0003899
• protein dimerization activity (IEA) | GO:0046983

Biological Process

• transcription (IEA) | GO:0006350

Domains

• ( PF00037 ) 4Fe-4S binding domain
• ( PF01193 ) RNA polymerase Rpb3/Rpb11 dimerisation domain
• ( PF01000 ) DNA-directed RNA polymerase, alpha subunit,

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.2%

TetraMine Data

TTHERM_00193849

WebApollo

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Tetrahymena Stock Center

• ( SD01935 ) Micronucleus: RPB3 coding replaced by GFP sequence, neo3 in 3’ flanking region. Heterozygous
• ( SD01936 ) Micronucleus: RPB3 coding replaced by GFP sequence, neo3 in 3’ flanking region. Heterozygous
• ( SD02653 ) Micronucleus: GFP replaces RPB3 coding, neo3 in 3’flanking region. homozygous
• ( SD02654 ) Micronucleus: GFP replaces RPB3 coding, neo3 in 3’ flanking region. homozygous
• ( SD01817 ) Macronucleus: Complete replacement of RPB3 with c-terminal HA tag and neo3 in 3’ flanking region

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_03659.1	9.997093137033517e-262	DNA-directed RNA polymerase II subunit (311 aa)
DictyBase	DDB_G0292244	9.997863464222902e-69	rpb3 on chromosome: 6 position 1368967 to 1370237
Oxytricha	Contig8173.0.g80	8.99739772315193e-58	RNA polymerase Rpb3/RpoA inser t domain
SGD	YIL021W	2.00017271388466e-38	RPB3 RNA polymerase II third l argest subunit B44, part of ce ntral core; similar to prokary otic alpha subunit
WormBase	WBGene00007971	3.9997566980151843e-38	locus:rpb-3 status:Partially _confirmed UniProt:Q93338 protein_id:CAB02268.2
Stentor Coeruleus	SteCoe_22357	3.975449735908647e-31

General Information

No Data fetched for General Information

Associated Literature

1. Ref:15470252: Mochizuki K, Gorovsky MA (2004) RNA polymerase II localizes in Tetrahymena thermophila meiotic micronuclei when micronuclear transcription associated with genome rearrangement occurs. Eukaryotic cell 3(5):1233-40
2. Ref:10704239: Larsen LK, Andreasen PH, Dreisig H, Palm L, Nielsen H, Engberg J, Kristiansen K (1999) Cloning and characterization of the gene encoding the highly expressed ribosomal protein l3 of the ciliated protozoan Tetrahymena thermophila. Evidence for differential codon usage in highly expressed genes. Cell biology international 23(8):551-60
3. Ref:2112240: Martindale DW (1990) A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of the prokaryotic RNA polymerase alpha subunit (rpoA). Nucleic acids research 18(10):2953-60

Sequences

>TTHERM_00193849(protein)
MEGIKLTLTNLEEFKCEFTLENCDISLANSLRRILLAEIETIAIHNVKVYENTSSLPDEY
IAHRLGLIPLNSAKVDDYNYIWNCNCQGECDRCTIHFDLRVSNFTNEVLEVTSLDLKQRQ
DHTDEDAVRPIKVYSILRDEYGNKREVGIPIIKLSKGQQIHFECEAQKGIGKMHSKWNPV
CISTFSVEPEIIFDQEIQNLDIEQKKALVNACPTKVFGINPHTNTLEVQDHMKCMYCEEC
VYKCAEDFQKPKLIKIDHKKDKFFFKVETTGVMKPVEVLRRGFSTLRQKLDFMREQIQEN
SNNQFEYGNE

>TTHERM_00193849(coding)
ATGGAAGGAATTAAATTAACTCTTACAAACCTCGAAGAATTCAAATGCGAATTTACCCTA
GAAAATTGCGATATCTCATTAGCTAATTCATTAAGAAGAATTCTTTTGGCTGAAATTGAA
ACAATAGCTATCCATAACGTTAAAGTATATGAGAATACATCTTCTCTTCCTGATGAATAC
ATAGCACATAGATTAGGTTTAATTCCTTTAAATTCTGCTAAAGTTGATGATTATAACTAT
ATTTGGAATTGCAATTGCCAAGGTGAATGCGATAGATGTACAATTCATTTTGATTTGAGA
GTGAGTAACTTTACAAATGAAGTCTTAGAAGTTACTTCTCTTGATCTTAAATAAAGATAG
GACCACACTGATGAAGATGCCGTTCGCCCTATAAAGGTATATTCAATTTTACGTGATGAA
TATGGAAATAAGAGAGAAGTAGGTATTCCTATTATCAAGCTATCTAAAGGACAATAAATT
CACTTTGAATGTGAAGCCTAGAAAGGTATCGGTAAAATGCATTCTAAATGGAATCCAGTA
TGTATTTCAACTTTCTCAGTTGAACCTGAAATTATTTTCGATCAAGAAATTTAAAATTTA
GATATTGAATAGAAGAAAGCTCTAGTAAATGCATGTCCTACTAAAGTATTTGGTATCAAT
CCTCATACCAATACTTTGGAAGTCTAAGATCACATGAAATGTATGTATTGTGAAGAATGT
GTTTACAAGTGTGCTGAAGACTTCTAAAAACCTAAATTAATCAAAATAGACCATAAAAAG
GATAAGTTTTTCTTCAAAGTCGAAACTACAGGAGTTATGAAGCCTGTTGAAGTGTTAAGA
AGAGGTTTTTCAACCTTGAGATAGAAGCTTGATTTTATGAGAGAATAAATCTAAGAAAAT
TCTAATAATTAATTTGAATATGGTAACGAGTGA

>TTHERM_00193849(gene)
ATGGAAGGAATTAAATTAACTCTTACAAACCTCGAAGAATTCAAATGCGAATTTACCCTA
GAAAATTGCGATATCTCATTAGCTAATTCATTAAGAAGAATTCTTTTGGCTGAAATTGAA
ACAATAGCTATCCATAACGTTAAAGTATATGAGAATACATCTTCTCTTCCTGATGAATAC
ATAGCACATAGATTAGGTTTAATTCCTTTAAATTCTGCTAAAGTTGATGATTATAACTAT
ATTTGGAATTGCAATTGCCAAGGTGAATGCGATAGATGTACAATTCATTTTGATTTGAGA
GTGAGTAACTTTACAAATGAAGTCTTAGAAGTTACTTCTCTTGATCTTAAATAAAGATAG
GACCACACTGATGAAGATGCCGTTCGCCCTATAAAGGTATATTCAATTTTACGTGATGAA
TATGGAAATAAGAGAGAAGTAGGTATTCCTATTATCAAGCTATCTAAAGGACAATAAATT
CACTTTGAATGTGAAGCCTAGAAAGGTATCGGTAAAATGCATTCTAAATGGAATCCAGTA
TGTATTTCAACTTTCTCAGTTGAACCTGAAATTATTTTCGATCAAGAAATTTAAAATTTA
GATATTGAATAGAAGAAAGCTCTAGTAAATGCATGTCCTACTAAAGTATTTGGTATCAAT
CCTCATACCAATACTTTGGAAGTCTAAGATCACATGAAATGTATGTATTGTGAAGAATGT
GTTTACAAGTGTGCTGAAGACTTCTAAAAACCTAAATTAATCAAAATAGACCATAAAAAG
GATAAGTTTTTCTTCAAAGTCGAAACTACAGGAGTTATGAAGCCTGTTGAAGTGTTAAGA
AGAGGTTTTTCAACCTTGAGATAGAAGCTTGATTTTATGAGAGAATAAATCTAAGAAAAT
TCTAATAATTAATTTGAATATGGTAACGAGTGA