Identifiers and Description

Gene Model Identifier

TTHERM_00283900

Standard Name

RNH13 (RNase H1.3)

Aliases

PreTt04929 | 25.m00393 | 3694.m00176

Description

RNase H family protein; localizes to micronucleus during conjugation

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Molecular Function

• RNA-DNA hybrid ribonuclease activity (IEA) | GO:0004523
• nucleic acid binding (IEA) | GO:0003676

Domains

• ( PF00075 ) ribonuclease HI

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.1%

TetraMine Data

TTHERM_00283900

WebApollo

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Tetrahymena Stock Center

• ( SD02710 ) Macronucleus: RNH1.3 with HA tag
• ( SD02711 ) Macronucleus: RNH1.3 with HA tag
• ( SD02712 ) Macronucleus: Neo3 knockout of RNH1.3
• ( SD02713 ) Macronucleus: Neo3 knockout of RNH1.3
• ( SD02714 ) Macronucleus: Neo3 knockout of RNH1.3
• ( SD02715 ) Macronucleus: Neo3 knockout of RNH1.3

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_09026.1	9.997093137033517e-262	hypothetical protein (695 aa)

General Information

Paragraph No	Gene Name	Paragraph Text
117	RNH13	HA-tagged RNase H1.3 localizes to early meiotic micronuclei. RNH13 gene knockout causes aberrant numbers of nuclei in exconjugants.

Associated Literature

1. Ref:SUPR000398

Sequences

>TTHERM_00283900(coding)
ATGTGCTATAAGTGCCTTCCTGATCATAGTCTCCATTCTGAATAGATTGTTTAGCTTGAT
AACTTAAGTGATAAAAAAAACTTGGATTGCCAAGAGAATATCAATAGCTCTATAGACAGC
AACAAAAATAATAAGCAGGGAGTCAGTCCACTTAAGGTAAATAGCGAATTCAATGGATCT
CCTTTTATGTTAAATAATCTAGCTAACTTAAAAATAAACTCCAGCTCATAGGAGCCTTCA
GATTAAAACGTAAAAAGAAGTAGTGTTAAAAATAGTTTTAGCAAAGTAAAAGAATTCAAT
TCAACATCTAACATAGAAGAAGAAATACTTGACTAGGGTCAAATCAAGCAAAAAAAGAAA
AAATCTAGCATTTCTCTTGATAAGCCAGAAAAATAAATACAACTTAACAATAATACACAA
AGTAGAAAATAAGAATTTAGAGATGTGTTTGTAATTAAAGAAGAAAATATGGATGATGAT
TATACAAACTGTAAAATTGAGAATGGTGGTAATTCTCATTTGAAAAACACTAATGCTTTA
CCTCAAAATGGAAAGAAATATTCTAAAGAGTCTGCTCAAACTAAAGTTTTTGGGACAGAT
ATGTTGTCACAAATTTCCAGACAAAAAGGTAGTAGTTCCTAAAAAAACATAAATCAGTTA
CTAAGTTAAGCTTCCACAGTATCTCCAAGCATCTAACCTGTTGAGAAGAGTATGATGGAT
GTGATTTCTAATACAACTAATTATTATCCATAATATCCCTTCTATCCTCCTCATAATCCT
TATGAAAATCCTTTCTAATTTAGCACTAATTTTAATCCAGGCCACTAAATGACTTATCCT
CATCCACATCCTTAAGGGTACCCACCAAATTAGGTAAATGCGCCTTTTTCTCACCTCTAT
AACCAGTAACATAATTTTAACAATATGCCTCCTTACTATCCTCCTACTCGTTCTTCTTAT
ATGCCAAGCTTTAACCATAATTATTAGTAGAACCACATGCTTTAAGAGTAAGAAACTAAA
GAATAATATCATACTATATCTGATTCCAGTTCTAGTGATAAATATTTTTCAAAAAAAGAT
AAATTAAGTAATGAAAGAAGTCTCTAAAGAAATTAAATGAAAAATGACAAAATAGATTTT
GTTTCATCTGATGAATCTTTGCAATAATAAGAATAGACAGCTACAGACTTTTTTTAAAAT
GGAGCTCTCTCTGAGTTGATTAAGCAAAACGAAAACATTCAAAAGATATGTGTTTATCTT
AGAACTTTAAATGACATTAAAATGTCAGTATGCACTAAAATTGAGGCCATGAAGAATAAA
TTCCTACAACAAATATAAATGAAAGAATAAGATTTCAGTTATGCATTTTTGGCAAATACT
TTCAACCAAAATAATAACTAAGTAGAAGAAAACTTAAAGAGCTACTTTGCAAAAATACAG
CAACTAAATTCCTTTTTTGAAAAGAATTAACTAAGCTACAACCCTTAAGCTGCTAGCAAA
TAGAAACTAAGCTAGATTGAATAAGAACTTGATAGAATGCTTTTTTTTACTATTGAGAAA
ATAGATTAGGAAACTTTAAATTAAACTAAACTAATCAAGTAAGAACATATTGACCCTTTC
AAAGATAATTCAGAGTATGAAGAATTAACATAATTTTATCAAAACTAACCTGCTAATATT
GATGCAATCAAAGACAAAATAAACTTTTATTCTTAGTGTTAGCTCGAATTTGATGGAACA
TCAAAAGGAAACCCTGGAAGATCTGGAGCTGGCTTTATCATCAAAGATTTATACACTGAG
AATAAATTACTTGAGTATTCAGTAGAAGTAGGAACAAAAACTATCAATTAATCTGAGTAT
CTAGCTTTAATCTATGGAATGTATACTTGCTATAAGCTAGGTATTAAGCAGCTTACAATT
TAAGGTGACTCTGAAGTTGTGATAAAACACATGTTAGGTGTTTACTAATGCAGAAGCTAA
CAGCTCAGAGAATACTATAATCTAGCTAAAATTTTAGATGAGCTTTTCCATTTTAAGGAC
TACGTTTAAGTAAAAAAAGAGCTTAACACTGAAGCTGATGCACTAGCCAATCTAGCACTT
AGTTGA

>TTHERM_00283900(protein)
MCYKCLPDHSLHSEQIVQLDNLSDKKNLDCQENINSSIDSNKNNKQGVSPLKVNSEFNGS
PFMLNNLANLKINSSSQEPSDQNVKRSSVKNSFSKVKEFNSTSNIEEEILDQGQIKQKKK
KSSISLDKPEKQIQLNNNTQSRKQEFRDVFVIKEENMDDDYTNCKIENGGNSHLKNTNAL
PQNGKKYSKESAQTKVFGTDMLSQISRQKGSSSQKNINQLLSQASTVSPSIQPVEKSMMD
VISNTTNYYPQYPFYPPHNPYENPFQFSTNFNPGHQMTYPHPHPQGYPPNQVNAPFSHLY
NQQHNFNNMPPYYPPTRSSYMPSFNHNYQQNHMLQEQETKEQYHTISDSSSSDKYFSKKD
KLSNERSLQRNQMKNDKIDFVSSDESLQQQEQTATDFFQNGALSELIKQNENIQKICVYL
RTLNDIKMSVCTKIEAMKNKFLQQIQMKEQDFSYAFLANTFNQNNNQVEENLKSYFAKIQ
QLNSFFEKNQLSYNPQAASKQKLSQIEQELDRMLFFTIEKIDQETLNQTKLIKQEHIDPF
KDNSEYEELTQFYQNQPANIDAIKDKINFYSQCQLEFDGTSKGNPGRSGAGFIIKDLYTE
NKLLEYSVEVGTKTINQSEYLALIYGMYTCYKLGIKQLTIQGDSEVVIKHMLGVYQCRSQ
QLREYYNLAKILDELFHFKDYVQVKKELNTEADALANLALS

>TTHERM_00283900(gene)
ATGTGCTATAAGTGCCTTCCTGATCATAGTCTCCATTCTGAATAGATTGTTTAGCTTGAT
AACTTAAGTGATAAAAAAAACTTGGATTGCCAAGAGAATATCAATAGCTCTATAGACAGC
AACAAAAATAATAAGCAGGGAGTCAGTCCACTTAAGGTAAATAGCGAATTCAATGGATCT
CCTTTTATGTTAAATAATCTAGCTAACTTAAAAATAAACTCCAGCTCATAGGAGCCTTCA
GATTAAAACGTAAAAAGAAGTAGTGTTAAAAATAGTTTTAGCAAAGTAAAAGAATTCAAT
TCAACATCTAACATAGAAGAAGAAATACTTGACTAGGGTCAAATCAAGCAAAAAAAGAAA
AAATCTAGCATTTCTCTTGATAAGCCAGAAAAATAAATACAACTTAACAATAATACACAA
AGTAGAAAATAAGAATTTAGAGATGTGTTTGTAATTAAAGAAGAAAATATGGATGATGAT
TATACAAACTGTAAAATTGAGAATGGTGGTAATTCTCATTTGAAAAACACTAATGCTTTA
CCTCAAAATGGAAAGAAATATTCTAAAGAGTCTGCTCAAACTAAAGTTTTTGGGACAGAT
ATGTTGTCACAAATTTCCAGACAAAAAGGTAGTAGTTCCTAAAAAAACATAAATCAGTTA
CTAAGTTAAGCTTCCACAGTATCTCCAAGCATCTAACCTGTTGAGAAGAGTATGATGGAT
GTGATTTCTAATACAACTAATTATTATCCATAATATCCCTTCTATCCTCCTCATAATCCT
TATGAAAATCCTTTCTAATTTAGCACTAATTTTAATCCAGGCCACTAAATGACTTATCCT
CATCCACATCCTTAAGGGTACCCACCAAATTAGGTAAATGCGCCTTTTTCTCACCTCTAT
AACCAGTAACATAATTTTAACAATATGCCTCCTTACTATCCTCCTACTCGTTCTTCTTAT
ATGCCAAGCTTTAACCATAATTATTAGTAGAACCACATGCTTTAAGAGTAAGAAACTAAA
GAATAATATCATACTATATCTGATTCCAGTTCTAGTGATAAATATTTTTCAAAAAAAGAT
AAATTAAGTAATGAAAGAAGTCTCTAAAGAAATTAAATGAAAAATGACAAAATAGATTTT
GTTTCATCTGATGAATCTTTGCAATAATAAGAATAGACAGCTACAGACTTTTTTTAAAAT
GGAGCTCTCTCTGAGTTGATTAAGCAAAACGAAAACATTCAAAAGATATGTGTTTATCTT
AGAACTTTAAATGACATTAAAATGTCAGTATGCACTAAAATTGAGGCCATGAAGAATAAA
TTCCTACAACAAATATAAATGAAAGAATAAGATTTCAGTTATGCATTTTTGGCAAATACT
TTCAACCAAAATAATAACTAAGTAGAAGAAAACTTAAAGAGCTACTTTGCAAAAATACAG
CAACTAAATTCCTTTTTTGAAAAGAATTAACTAAGCTACAACCCTTAAGCTGCTAGCAAA
TAGAAACTAAGCTAGATTGAATAAGAACTTGATAGAATGCTTTTTTTTACTATTGAGAAA
ATAGATTAGGAAACTTTAAATTAAACTAAACTAATCAAGTAAGAACATATTGACCCTTTC
AAAGATAATTCAGAGTATGAAGAATTAACATAATTTTATCAAAACTAACCTGCTAATATT
GATGCAATCAAAGACAAAATAAACTTTTATTCTTAGTGTTAGCTCGAATTTGATGGAACA
TCAAAAGGAAACCCTGGAAGATCTGGAGCTGGCTTTATCATCAAAGATTTATACACTGAG
AATAAATTACTTGAGTATTCAGTAGAAGTAGGAACAAAAACTATCAATTAATCTGAGTAT
CTAGCTTTAATCTATGGAATGTATACTTGCTATAAGCTAGGTATTAAGCAGCTTACAATT
TAAGGTGACTCTGAAGTTGTGATAAAACACATGTTAGGTGTTTACTAATGCAGAAGCTAA
CAGCTCAGAGAATACTATAATCTAGCTAAAATTTTAGATGAGCTTTTCCATTTTAAGGAC
TACGTTTAAGTAAAAAAAGAGCTTAACACTGAAGCTGATGCACTAGCCAATCTAGCACTT
AGTTGA