Identifiers and Description

Gene Model Identifier

TTHERM_00105430

Standard Name

PRF1 (PRoFilin)

Aliases

PreTt23043 | 7.m00567

Description

Profilin family protein; both inhibits and enhances actin polymerization; co-localizes with actin in the division furrow

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• actin cytoskeleton (IEA) | GO:0015629
• cleavage furrow (IDA) | GO:0032154 | Ref:1478923
• actomyosin contractile ring (IDA) | GO:0005826 | Ref:1478923

Molecular Function

• actin monomer binding (ISS) | GO:0003785 | Ref:10767551
• actin binding (IEA) | GO:0003779

Biological Process

• cytokinesis (IMP) | GO:0000910 | Ref:15132168
• cytoskeleton organization (IEA) | GO:0007010
• nucleus localization (IMP) | GO:0051647 | Ref:15132168
• phagocytosis (IMP) | GO:0006909 | Ref:15132168
• regulation of actin filament polymerization (ISS) | GO:0030833 | Ref:15132168

Domains

• ( PF00235 ) Profilin

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 11.9%

TetraMine Data

TTHERM_00105430

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_00377.1	3.999498296699862e-96	profilin family protein (153 a a)
Oxytricha	Contig22514.0.g15	9.995317835403734e-19	Profilin
WormBase	WBGene00003990	0.0000000400154582995175	locus:pfn-2 Profilin-2 sta tus:Confirmed UniProt:Q20025 protein_id:CCD62785.1
DictyBase	DDB_G0271142	0.00000009004106436083642	proC on chromosome: 2 position 57583 to 57963
SGD	YOR122C	0.00003998524687536623	PFY1 Profilin, binds actin, ph osphatidylinositol 4,5-bisphos phate, and polyproline regions ; involved in cytoskeleton org anization; required for normal timing of actin polymerizatio n in response to thermal stres s

General Information

No Data fetched for General Information

Associated Literature

1. Ref:15132168: Wilkes DE, Otto JJ (2004) Profilin functions in cytokinesis, nuclear positioning, and stomatogenesis in Tetrahymena thermophila. The Journal of eukaryotic microbiology 50(4):252-62
2. Ref:11942614: Numata O, Gonda K (2001) Determination of division plane and organization of contractile ring in Tetrahymena. Cell structure and function 26(6):593-601
3. Ref:10767551: Wilkes DE, Otto JJ (2000) Molecular cloning of profilin from Tetrahymena thermophila. Gene 246(1-2):295-301
4. Ref:1478923: Edamatsu M, Hirono M, Watanabe Y (1992) Tetrahymena profilin is localized in the division furrow. Journal of biochemistry 112(5):637-42
5. Ref:1902095: Edamatsu M, Hirono M, Takemasa T, Watanabe Y (1991) The primary structure of Tetrahymena profilin. Biochemical and biophysical research communications 175(2):543-50
6. Ref:2383275: Edamatsu M, Hirono M, Watanabe Y (1990) Purification and characterization of Tetrahymena profilin. Biochemical and biophysical research communications 170(2):957-62

Sequences

>TTHERM_00105430(coding)
ATGTCTGGTTGGGATCAATATATCTAATATCTTTTGGCTGATAAGAAGTGCGAATCTGCC
TACATCCTCGGTAAGGATCATGCTAACATTTGGGCTTGCTCTACTGGTATTGCTGCTCTT
CCTACTTACTAAATCAATGTCGAAGGTAAAAACTTCAATGTTAACGAAGCTGAGCTCTTG
GTTAAGGCTTTGAAGAACAACGGTGTTCCCACTGATCCTAATGTTGGTTTAAGAATTAAG
AATGAAAAGTACTACACTGTCAGATTCGACGCTGATGCTGGTACCTGGTATCTTAAGAAG
GATTAAGGTGGTGCTTGCATTGCCGTTACCAAGTAAGCTTTAGTTATTGGTACTTTCAAA
AACGACATCAAAATGACTAACGGTCAACCTCAAAATCCTGGTGCCGTCAACGCTGCTTGT
GAAAAGCTCGCTGAATCCTTAAAGTCCGCTAACTACTGA

>TTHERM_00105430(protein)
MSGWDQYIQYLLADKKCESAYILGKDHANIWACSTGIAALPTYQINVEGKNFNVNEAELL
VKALKNNGVPTDPNVGLRIKNEKYYTVRFDADAGTWYLKKDQGGACIAVTKQALVIGTFK
NDIKMTNGQPQNPGAVNAACEKLAESLKSANY

>TTHERM_00105430(gene)
ATGTCTGGTTGGGATCAATATATCTAATATCTTTTGGCTGATAAGAAGTGCGAATCTGCC
TACATCCTCGGTAAGGATCATGCTAACATTTGGGCTTGCTCTACTGGTATTGCTGCTGTA
AGATATATTAGATTTTGCTTCTTTTTGGCCTTTTTTGCCATTTTAGTCACTCTATGATTT
TGTGACTTCAATATTAATTTCATGTATTAATTTTCCTTTTTACTAAACAATCTAGCTTCC
TACTTACTAAATCAATGTCGAAGGTAAAAACTTCAATGTTAACGAAGCTGAGCTCTTGGT
TAAGGGTAATTTTTCTTTTAATTCATTAACTATCTCATATAATTGGCTGATTATCTTCAA
GCTTCTTCTCTCAAAAACTCTTTCATTCCTATGAAAATTATTAGTGTTTAATATTTTTAA
GATCCGAAGAACAATAAATAAATAAATAATATCCTTCGAACAAAAATTAGCAAACCTTCA
AATCATTTAATCAATAAATAAATAAATATATAAGTACATAGCAACTATGCAACTAACTAA
TAAGGAATGAAGTACTCAATTTTTAACAAATATTTGCCAATATTCATCAAATTTTGATTA
GCTTTCCTCTTTAATATTTACTAATATTAATAATAATATTCTCTTTCGTTAAATATTTTA
GCTTTGAAGAACAACGGTGTTCCCACTGATCCTAATGTTGGTTTAAGAATTAAGAATGAA
AAGTACTACACTGTCAGATTCGACGCTGATGCTGGTACCTGGTATCTTAAGAAGGTATAT
AATTTATTATTATTTAAGTTAGCAAATTATTCAAAGCTATAGCTATTAGGTGTCTTAAAA
AAATAAACTAGCCAGCCATTTATTTATAATATTTGTTTTCATGACTGATATTAAGATATT
TTAGAAAATAAAGCACCAAGCACTCAAAAAATAAAATAATGCAAAAATTAATATTATTTT
TCAAAAAATAATTTAGGATTAAGGTGGTGCTTGCATTGCCGTTACCAAGTAAGCTTTAGT
TATTGGTACTTTCAAAAACGACATCAAAATGACTAACGGTCAACCTCAAAATCCTGGTGC
CGTCAACGCTGCTTGTGAAAAGCTCGCTGAATCCTTAAAGTCCGCTAACTACTGA