Identifiers and Description

Gene Model Identifier

TTHERM_00865290

Standard Name

PPO1 (protein phosphatase 1 (PP1) ortholog)

Aliases

PreTt29159 | 154.m00117 | 3751.m00463

Description

PPO1 serine/threonine-protein phosphatase PP1; Glc7-like protein phosphatase. !!! Note that TTHERM_00865290 and TTHERM_00865300 are parts of a single gene !!!

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Biological Process

• histone H3-S10 phosphorylation involved in chromosome condensation (EXP) | GO:2000775 | Ref:22426537
• exit from mitosis (EXP) | GO:0010458 | Ref:22426537
• mitotic nuclear division (EXP) | GO:0007067 | Ref:22426537

Domains

No Data fetched for Domains

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.4%

TetraMine Data

TTHERM_00865290

WebApollo

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Tetrahymena Stock Center

• ( SD01771 ) Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
• ( SD02818 ) Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
• ( SD02819 ) Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
• ( SD02820 ) Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
• ( SD02821 ) Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
• ( SD02196 ) Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
• ( SD02197 ) Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
• ( SD02539 ) Macronucleus: Ppo1p with n-terminal V5 tag, cells very sick, not used in paper
• ( SD02540 ) Macronucleus: Ppo1p with n-terminal V5 tag
• ( SD02732 ) Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag.
• ( SD02733 ) Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Stentor Coeruleus	SteCoe_9187	0
Tetrahymena borealis	EI9_19695.1	9.997093137033517e-262	serine/threonine protein phosp hatase PP1 (315 aa)
WormBase	WBGene00001748	1.9991691033935208e-179	locus:gsp-2 Serine/threonine -protein phosphatase PP1-beta status:Confirmed UniProt:P 48727 protein_id:CCD61828.1
DictyBase	DDB_G0275619	9.001500394647905e-175	pppB on chromosome: 2 position 6106886 to 6107851
SGD	YER133W	9.998061997456677e-175	GLC7 Type 1 serine/threonine p rotein phosphatase catalytic s ubunit, involved in many proce sses (eg: glycogen metabolism, sporulation, mitosis); accumu lates at mating projections by interaction with Afr1p; inter acts with many regulatory subu nits
Oxytricha	Contig11781.0.0.g15	3.0005817332825333e-174	Calcineurin-like phosphoestera se

General Information

No Data fetched for General Information

Associated Literature

1. Ref:22426537: Song X, Bowen J, Miao W, Liu Y, Gorovsky MA (2012) The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes & development 26(6):615-29
2. Ref:10199406: Wei Y, Yu L, Bowen J, Gorovsky MA, Allis CD (1999) Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97(1):99-109
3. Ref:9636175: Wei Y, Mizzen CA, Cook RG, Gorovsky MA, Allis CD (1998) Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena. Proceedings of the National Academy of Sciences of the United States of America 95(13):7480-4

Sequences

>TTHERM_00865290(coding)
ATGGACGGAAAAACAGTCATTGATATTGATGATATCCTATCAAAATTGATAGATCCAAAG
CAAAGTAGAGCAGCTAAAAGTTAGGTTTTGGCTGAAAGCGAAATTAGGGCTGTTTGCATG
AAGTCAAGAGAAATCTTTTTGCAATAGCCAATGCTTTTAGAGTTAGAAGCTCCCATTAAA
ATTTGTGGTGATGTTCATGGCCAATTCCCTGATTTACTTAGAATTTTCGAATACGGAGGA
TTCCCTCCTGATGCCAATTATCTCTTTTTAGGAGATTACGTTGATAGAGGCAAATAATCA
CTAGAAACAATTTGTTTGCTCTTAGCTTATAAAATCAAATTTCCTGAGAATTTCTTCTTG
CTTCGTGGTAACCACGAATGCTCCTCCATTAATCGTATATATGGTTTCTATGATGAATGT
AAAAGAAGATATAATATAAAGCTCTGGAAAATTTTTACTGACTGCTTTAATTGCCTTCCA
GTCTGTGCAGTGATAGATGAAAAAATTATTTGTATGCACGGAGGTCTTTCACCTTCTCCT
GAATTTAATAATATAGAATCTATTCATCGTATAACACGTCCAATTGAAGTTCCTGACTAA
GGTTTATTATGCGATCTATTGTGGAGTGACCCTGAAAAAGATATTCTTGGTTGGGGTGAT
AATGAAAGAGGTGTTAGTTATATATTTGGACCAGATGAGGTCTCGAAATTCCTAAAGAAA
TAAGATTTAGATTTAATATGTAGAGCACATTAGGTAGTCGAAGATGGATATGAGTTCTTC
GCAAAAAGATAGTTAGTCACAATCTTTTCAGCTCCTAATTATTGTGGTGAATTTGATAAT
TGCGGTGCTTTAATGTCTGTAGATGAGACTTTAATGTGTAGCTTCTAAATATTAAAACCT
GCAGATAAAAAATCTTAAACTTAACGTCCTAAAACTCCAAAGTTTGTCAATTGA

>TTHERM_00865290(protein)
MDGKTVIDIDDILSKLIDPKQSRAAKSQVLAESEIRAVCMKSREIFLQQPMLLELEAPIK
ICGDVHGQFPDLLRIFEYGGFPPDANYLFLGDYVDRGKQSLETICLLLAYKIKFPENFFL
LRGNHECSSINRIYGFYDECKRRYNIKLWKIFTDCFNCLPVCAVIDEKIICMHGGLSPSP
EFNNIESIHRITRPIEVPDQGLLCDLLWSDPEKDILGWGDNERGVSYIFGPDEVSKFLKK
QDLDLICRAHQVVEDGYEFFAKRQLVTIFSAPNYCGEFDNCGALMSVDETLMCSFQILKP
ADKKSQTQRPKTPKFVN

>TTHERM_00865290(gene)
ATGGACGGAAAAACAGTCATTGATATTGATGATATCCTATCAAAATTGATAGATCCAAAG
CAAAGTAGAGCAGCTAAAAGTTAGGTTTTGGCTGAAAGCGAAATTAGGGCTGTTTGCATG
AAGTCAAGAGAAATCTTTTTGCAATAGCCAATGCTTTTAGAGTTAGAAGCTCCCATTAAA
ATTTGTGGTAATTAGAAGCCTTATTTTTCGATAAAATTAAAATAAATGTTTTTAGGGTAA
CATTAAGGCAATAAAAAACATTTAAATAATAGGTGATGTTCATGGCCAATTCCCTGATTT
ACTTAGAATTTTCGAATACGGAGGATTCCCTCCTGATGCCAATTATCTCTTTTTAGGAGA
TTACGTTGATAGAGGCAAATAATCACTAGAAACAATTTGTTTGCTCTTAGCTTATAAAAT
CAAATTTCCTGAGAATTTCTTCTTGCTTCGTGGTAACCACGAATGCTCCTCCATTAATCG
TATATATGGTTTCTATGATGAATGTAAGAGTTTTTATTTTTAAGGAGAAATTAATTTCTG
CTCAGAGCAGCCTAAATTCGTGTTTTCAAAAATTAGAAGAAAATGATTGAAAGTTTTTTA
AATAAGAAGGATAAATAACAGAAAAAATAATAGATTATTTCTAGCTAATCACACTATTTT
CAATTAAAAAAAGCAATTATATTTGAAATATTTCTCATTAAGTCAAATAAATTTGCTCAT
TTTTAGCTAATAACTTATTTTGTAATTTAATTGTATATGAAATAAAAAGGTAAAAGAAGA
TATAATATAAAGCTCTGGAAAATTTTTACTGACTGCTTTAATTGCCTTCCAGTCTGTGCA
GTGATAGATGAAAAAATTATTTGTATGCACGGAGGTCTTTCACCTTCTCCTGAATTTAAT
AATATAGAATCTATTCATCGTATAACACGTCCAATTGAAGTTCCTGACTAAGGTTACTAA
TTAACTAACTATCTTACCAAACAAACATCAAAATTTATTTTCTCTACTTTCAAAATTAAC
TGATAGAATTTTTAAAAAATGAATATTTCAAGATCTTTCTAGTTAGCAAAACAAGGAAAT
AGCACATGTTTATTCTTTCATACATACTAAATTGATGTTAATTATTTTTTTAGTGCAAAC
ACTATCTTAAAGAAAGATTAATTGTCACTTAAGATTCATAAATTTAGCGAAGCAAAAAAT
ATGAAGCAAAATAAAGTATTGAATAAATCAGATTCAAGTGAAATAGATTATTCAACTTAT
TTTTATCACAAACTTTAACCAGAATATTATCTAACTAAATGTTACTTCCAATAGAATAAT
TCATACTTAAAAATTTAGTGATGTTCAATAATATTTGTATTATCAATAAAAAATAATAAT
GGATAACTAAACTAAAGGAAATTAAATCATATTTAAGATTTACAAATATATAAAAATAAG
TAAGCATATATATAATAATAAAGTAGCTTCAACCAACATCTAACAAAAAAGTTAAAATTT
AGAGAAAATAAATATATTTTAATCACTTTAAGTTTGACTAAAAATAATAATTATTAAAAT
TTAAAATAGGTTTATTATGCGATCTATTGTGGAGTGACCCTGAAAAAGATATTCTTGGTT
GGGGTGATAATGAAAGAGGTGTTAGTTATATATTTGGACCAGATGAGGTCTCGAAATTCC
TAAAGAAATAAGATTTAGATTTAATATGTAGAGCACATTAGGTAGTCGAAGATGGATATG
AGTTCTTCGCAAAAAGATAGTTAGTCACAATCTTTTCAGCTCCTAATTATTGTGGTGAAT
TTGATAATTGCGGTAAATTATTTTTTAATAAGCAAATAATGGTATTAAAATTTGACATTT
TATCTATAATTTTATTATTATTTAATTAGGTGCTTTAATGTCTGTAGATGAGACTTTAAT
GTGTAGCTTCTAAATATTAAAACCTGCAGATAAAAAATCTTAAACTTAACGTCCTAAAAC
TCCAAAGGTAATTATTTAGAAGTATTTTAAGTATAAGTTAATGCAAAATTTATTTTATAA
AAAAAGTTTGTCAATTGA