Identifiers and Description

Gene Model Identifier

TTHERM_00684560

Standard Name

ORC2 (origin recognition complex subunit 2)

Aliases

PreTt18169 | 104.m00101 | 3732.m00015

Description

hypothetical protein

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• nucleus (IEA) | GO:0005634
• origin recognition complex (IEA) | GO:0000808

Biological Process

• DNA replication (IEA) | GO:0006260

Domains

• ( PF00466 ) ribosomal protein L10
• ( PF02180 ) Bcl-2 homology region 4
• ( PF04084 ) Origin recognition complex subunit 2

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.3%

TetraMine Data

TTHERM_00684560

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_17345.1	6.002643599093603e-161	hypothetical protein (754 aa)
Oxytricha	Contig6689.0.g11	3.998693556225637e-29	Origin recognition complex sub unit 2
DictyBase	DDB_G0269894	0.0000000000002999981760818895	orcB on chromosome: 1 position 3810811 to 3812262
SGD	YBR060C	0.000000000029994712494008525	ORC2 Subunit of the origin rec ognition complex, which direct s DNA replication by binding t o replication origins and is a lso involved in transcriptiona l silencing; interacts with Sp p1p and with trimethylated his tone H3; phosphorylated by Cdc 28p
WormBase	WBGene00003882	0.00000019998969434520019	locus:orc-2 Origin recogniti on complex subunit 2 status: Confirmed UniProt:Q21037 p rotein_id:CAA85415.1

General Information

Paragraph No	Gene Name	Paragraph Text
66	ORC2	Gene Information: TTHERM_00684560 against human ORC subunit 2 protein e-value= 8e-16 Reverse BLAST of NP_006181.1 against TGD: TTHERM_00684560 hypothetical protein e-value= 8e-15.
67	ORC2	The conserved Origin Recognition Complex (ORC) determines the sites for replication initiation in eukaryotic chromosomes and serves as a scaffold for pre-replicative complex (pre-RC) assembly. Although ORC subunits are conserved in eukaryotes, the cis-acting DNA sequence requirements for replicator function are not.
68	ORC2	Nucleic Acid Interactions: Orc2p bound to streptavidin sepharose in 26T RNA aptamer-tagged strains (TD152 and MM202, respectively)
69	ORC2	Protein Interaction Partners: Tetrahymena thermophila ORC physically associates with Orc1p in western blotting (native gel electrophoresis and immunoprecipitation analyses. Western blotting was similarly used to monitor the migration of nuclease-treated ORC complexes under native EMSA gel conditions. The mobility of Orc1p and Orc2p increased following MNase and RNase A treatment, but was unaltered by DNase I (Figure 3D). Orc1p and Orc2p co-migrated under all conditions, suggesting that they remain associated after the RNA is destroyed.
70	ORC2	Biochemical Activities: Consistent with previous analyses of Orc2p/Tt-p69 and histone H3 (Mohammad et al, 2003), ∼50% of Orc2 was rendered soluble by DNase I. TtORC also crossreacts with a rabbit polyclonal antibodies raised against Xenopus laevis / Orc2p.
71	ORC2	Regulation & Expression: Orc2p crossreactive subunit, Tt-p69, localizes to the macronucleus during vegetative S phase. Cell cycle regulated Maximal protein expression at G1/S border

Associated Literature

1. Ref:18007594: Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60
2. Ref:12832485: Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17

Sequences

>TTHERM_00684560(coding)
ATGCAAAATAATTAACAGGAAAGATTGGAATTCTAGGAAAAATATGGTATTTAAAAGGAC
TATATTTCTTTATCCAAAGAAGATAAAAAGAAATAAGCTGCAGATGAAGTCATTGAGTTT
AACAATAAAAACTTGATTTCTTATTTAGAAGATAAATATTAAGTAGAAGACACTGAAAAA
GTAAAAGAAGACAAAGCTAAAGCAATTGTGAAATCATGTTTTATGATACAATCAGGATTT
AATGTTTGCTTACATGGTTATGGCTCTAAGAGACAAATTATTTAAGAAATATCAGATTAA
TTTATTAATTACCCTAAATTAATCATTTAAGGATATTCAGCAGCTACTTAAATATAGCAA
ATTCTATAAAAACTAAAGTACATTGTGGTAAAGATTACTGGGTAAAACTCTGGTTAAAAG
AGAATTGAAGGAATATTAGAGTATCTAAAATAATAAGAGGAAAATATGGGAAAAATTTCC
TCTTAAGTACCTTTTATTTTAATTCTATTTCATAATATAGATTCTAAATAATTTATTGAG
TAAGATTCTGTCAAAATTTTATCTGCTGTACTAAATTTACCATATATAAAAGCTGTTTGT
AGTATTGATCATGTGAAGTATCCAATTATTCTGACTTCTAAAGTTTTAGAGTCATTTAAT
TTTAGTTTCATAGAAGTCCATACTCATTAATGCTATCTACATGAATTCAAAGTTTCCTAA
AATACATTTAACTTAAAAAAAGAAAAAGAAGGAGAATCTCTTAAATATATTTTTAGAAGT
CTGACAAAAGTTTAAAAAGAAATAATATAGTTTGTTGCAAAATATATTTAAGATAAATTA
GATTAAAATCCAGGAACAGCTATTTAAATAAATGGATTAACTGAAGATGGATAGATTATT
GGTTTAACTTTAAAAGAGTTATTAGAAATGCTAATTGATGAAATGATTTTGAACAGTGAG
TAGCAGTTAAAAGAAAATTTACTTGAAATACTTGATCACAAAGTTATAATTTCAATAGAA
AACTAGTAAAAAACTTACTATACAATGAATTTCAGCAGACAAATTTTAGAAAAAATTGTC
AACAACAAGTTGGAGGATGGAGAAGAAGATTAGGAGCAGGAAGAAGAGGAAGAAAACAAT
TCAGTAAAAGATATGGAAGAAGAGAATTAAATAATATCTAACAGCTAAAACAGTTCTCAA
AGTGAAGATGAAGATGTTAAAGGTAAAAAAAAATATAGATAAACAAGAAGAGAAGGTAAA
AAGAAAAAAAATAGTAGTGAGAGCTTATCAGATTCAGAAAATGAAAAACATAAGTTTAAT
AAGAAAACTTCTGGAATTTTGAAAACTAAAAATAAGTAATAAAAGAATAAAAAAAGTAGC
TTTACTTCATAAAGTAGTGAAGAAGATGGTGATGATGATAGCTATTAAAATTGA

>TTHERM_00684560(protein)
MQNNQQERLEFQEKYGIQKDYISLSKEDKKKQAADEVIEFNNKNLISYLEDKYQVEDTEK
VKEDKAKAIVKSCFMIQSGFNVCLHGYGSKRQIIQEISDQFINYPKLIIQGYSAATQIQQ
ILQKLKYIVVKITGQNSGQKRIEGILEYLKQQEENMGKISSQVPFILILFHNIDSKQFIE
QDSVKILSAVLNLPYIKAVCSIDHVKYPIILTSKVLESFNFSFIEVHTHQCYLHEFKVSQ
NTFNLKKEKEGESLKYIFRSLTKVQKEIIQFVAKYIQDKLDQNPGTAIQINGLTEDGQII
GLTLKELLEMLIDEMILNSEQQLKENLLEILDHKVIISIENQQKTYYTMNFSRQILEKIV
NNKLEDGEEDQEQEEEEENNSVKDMEEENQIISNSQNSSQSEDEDVKGKKKYRQTRREGK
KKKNSSESLSDSENEKHKFNKKTSGILKTKNKQQKNKKSSFTSQSSEEDGDDDSYQN

>TTHERM_00684560(gene)
ATGCAAAATAATTAACAGGAAAGATTGGAATTCTAGGAAAAATATGGTATTTAAAAGGAC
TATATTTCTTTATCCAAAGAAGATAAAAAGAAATAAGCTGCAGATGAAGTCATTGAGTTT
AACAATAAAAACTTGATTTCTTATTTAGAAGATAAATATTAAGTAGAAGACACTGAAAAA
GTAAAAGAAGACAAAGCTAAAGCAATTGTGAAATCATGTTTTATGATACAATCAGGATTT
AATGTTTGCTTACATGGTTATGGCTCTAAGAGACAAATTATTTAAGAAATATCAGATTAA
TTTATTAATTACCCTAAATTAATCATTTAAGGATATTCAGCAGCTACTTAAATATAGCAA
ATTCTATAAAAACTAAAGTACATTGTGGTAAAGATTACTGGGTAAAACTCTGGTTAAAAG
AGAATTGAAGGAATATTAGAGTATCTAAAATAATAAGAGGAAAATATGGGAAAAATTTCC
TCTTAAGTACCTTTTATTTTAATTCTATTTCATAATATAGATTCTAAATAATTTATTGAG
TAAGATTCTGTCAAAATTTTATCTGCTGTACTAAATTTACCATATATAAAAGCTGTTTGT
AGTATTGATCATGTGAAGTATCCAATTATTCTGACTTCTAAAGTTTTAGAGTCATTTAAT
TTTAGTTTCATAGAAGTCCATACTCATTAATGCTATCTACATGAATTCAAAGTTTCCTAA
AATACATTTAACTTAAAAAAAGAAAAAGAAGGAGAATCTCTTAAATATATTTTTAGAAGT
CTGACAAAAGTTTAAAAAGAAATAATATAGTTTGTTGCAAAATATATTTAAGATAAATTA
GATTAAAATCCAGGAACAGCTATTTAAATAAATGGATTAACTGAAGATGGATAGATTATT
GGTTTAACTTTAAAAGAGTTATTAGAAATGCTAATTGATGAAATGATTTTGAACAGTGAG
TAGCAGTTAAAAGAAAATTTACTTGAAATACTTGATCACAAAGTTATAATTTCAATAGAA
AACTAGTAAAAAACTTACTATACAATGAATTTCAGCAGACAAATTTTAGAAAAAATTGTC
AACAACAAGTTGGAGGATGGAGAAGAAGATTAGGAGCAGGAAGAAGAGGAAGAAAACAAT
TCAGTAAAAGATATGGAAGAAGAGAATTAAATAATATCTAACAGCTAAAACAGTTCTCAA
AGTGAAGATGAAGATGTTAAAGGTAAAAAAAAATATAGATAAACAAGAAGAGAAGGTAAA
AAGAAAAAAAATAGTAGTGAGAGCTTATCAGATTCAGAAAATGAAAAACATAAGTTTAAT
AAGAAAACTTCTGGAATTTTGAAAACTAAAAATAAGTAATAAAAGAATAAAAAAAGTAGC
TTTACTTCATAAAGTAGTGAAGAAGATGGTGATGATGATAGCTATTAAAATTGA