Identifiers and Description
Gene Model Identifier
TTHERM_00684560Standard Name
ORC2 (origin recognition complex subunit 2)Aliases
PreTt18169 | 104.m00101 | 3732.m00015Description
origin recognition complex subunit 2Genome Browser (Macronucleus)
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Genome Browser (Micronucleus)
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Gene Ontology Annotations
Cellular Component
- nucleus (IEA) | GO:0005634
- origin recognition complex (IEA) | GO:0000808
Biological Process
- DNA replication (IEA) | GO:0006260
Domains
Gene Expression Profile
Change in Gene Expression
TMHMM
TMHMM
Signal Peptide
Signal Peptide Present?: No
Presense Probability: 10.3%
TetraMine Data
TTHERM_00684560
WebApollo
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Tetrahymena Stock Center
No Data fetched for StockID
Homologs (v.2006 protein sequences)
Source Identifier Score Description Tetrahymena borealis EI9_17345.1 6.002643599093603e-161 hypothetical protein (754 aa)
Oxytricha Contig6689.0.g11 3.998693556225637e-29 Origin recognition complex sub
unit 2
DictyBase DDB_G0269894 0.0000000000002999981760818895 orcB on chromosome: 1 position
3810811 to 3812262
SGD YBR060C 0.000000000029994712494008525 ORC2 Subunit of the origin rec
ognition complex, which direct
s DNA replication by binding t
o replication origins and is a
lso involved in transcriptiona
l silencing; interacts with Sp
p1p and with trimethylated his
tone H3; phosphorylated by Cdc
28p
WormBase WBGene00003882 0.00000019998969434520019 locus:orc-2 Origin recogniti
on complex subunit 2 status:
Confirmed UniProt:Q21037 p
rotein_id:CAA85415.1
General Information
Paragraph No Gene Name Paragraph Text 66 ORC2 Gene Information: TTHERM_00684560 against human ORC subunit 2 protein e-value= 8e-16 Reverse BLAST of NP_006181.1 against TGD: TTHERM_00684560 hypothetical protein e-value= 8e-15. 67 ORC2 The conserved Origin Recognition Complex (ORC) determines the sites for replication initiation in eukaryotic chromosomes and serves as a scaffold for pre-replicative complex (pre-RC) assembly. Although ORC subunits are conserved in eukaryotes, the cis-acting DNA sequence requirements for replicator function are not. 68 ORC2 Nucleic Acid Interactions: Orc2p bound to streptavidin sepharose in 26T RNA aptamer-tagged strains (TD152 and MM202, respectively) 69 ORC2 Protein Interaction Partners: Tetrahymena thermophila ORC physically associates with Orc1p in western blotting (native gel electrophoresis and immunoprecipitation analyses. Western blotting was similarly used to monitor the migration of nuclease-treated ORC complexes under native EMSA gel conditions. The mobility of Orc1p and Orc2p increased following MNase and RNase A treatment, but was unaltered by DNase I (Figure 3D). Orc1p and Orc2p co-migrated under all conditions, suggesting that they remain associated after the RNA is destroyed. 70 ORC2 Biochemical Activities: Consistent with previous analyses of Orc2p/Tt-p69 and histone H3 (Mohammad et al, 2003), ∼50% of Orc2 was rendered soluble by DNase I. TtORC also crossreacts with a rabbit polyclonal antibodies raised against Xenopus laevis / Orc2p. 71 ORC2 Regulation & Expression: Orc2p crossreactive subunit, Tt-p69, localizes to the macronucleus during vegetative S phase. Cell cycle regulated Maximal protein expression at G1/S border
Associated Literature
- Ref:18007594: Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60
- Ref:12832485: Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17
Sequences
>TTHERM_00684560(coding)
ATGCAAAATAATTAACAGGAAAGATTGGAATTCTAGGAAAAATATGGTATTTAAAAGGAC
TATATTTCTTTATCCAAAGAAGATAAAAAGAAATAAGCTGCAGATGAAGTCATTGAGTTT
AACAATAAAAACTTGATTTCTTATTTAGAAGATAAATATTAAGTAGAAGACACTGAAAAA
GTAAAAGAAGACAAAGCTAAAGCAATTGTGAAATCATGTTTTATGATACAATCAGGATTT
AATGTTTGCTTACATGGTTATGGCTCTAAGAGACAAATTATTTAAGAAATATCAGATTAA
TTTATTAATTACCCTAAATTAATCATTTAAGGATATTCAGCAGCTACTTAAATATAGCAA
ATTCTATAAAAACTAAAGTACATTGTGGTAAAGATTACTGGGTAAAACTCTGGTTAAAAG
AGAATTGAAGGAATATTAGAGTATCTAAAATAATAAGAGGAAAATATGGGAAAAATTTCC
TCTTAAGTACCTTTTATTTTAATTCTATTTCATAATATAGATTCTAAATAATTTATTGAG
TAAGATTCTGTCAAAATTTTATCTGCTGTACTAAATTTACCATATATAAAAGCTGTTTGT
AGTATTGATCATGTGAAGTATCCAATTATTCTGACTTCTAAAGTTTTAGAGTCATTTAAT
TTTAGTTTCATAGAAGTCCATACTCATTAATGCTATCTACATGAATTCAAAGTTTCCTAA
AATACATTTAACTTAAAAAAAGAAAAAGAAGGAGAATCTCTTAAATATATTTTTAGAAGT
CTGACAAAAGTTTAAAAAGAAATAATATAGTTTGTTGCAAAATATATTTAAGATAAATTA
GATTAAAATCCAGGAACAGCTATTTAAATAAATGGATTAACTGAAGATGGATAGATTATT
GGTTTAACTTTAAAAGAGTTATTAGAAATGCTAATTGATGAAATGATTTTGAACAGTGAG
TAGCAGTTAAAAGAAAATTTACTTGAAATACTTGATCACAAAGTTATAATTTCAATAGAA
AACTAGTAAAAAACTTACTATACAATGAATTTCAGCAGACAAATTTTAGAAAAAATTGTC
AACAACAAGTTGGAGGATGGAGAAGAAGATTAGGAGCAGGAAGAAGAGGAAGAAAACAAT
TCAGTAAAAGATATGGAAGAAGAGAATTAAATAATATCTAACAGCTAAAACAGTTCTCAA
AGTGAAGATGAAGATGTTAAAGGTAAAAAAAAATATAGATAAACAAGAAGAGAAGGTAAA
AAGAAAAAAAATAGTAGTGAGAGCTTATCAGATTCAGAAAATGAAAAACATAAGTTTAAT
AAGAAAACTTCTGGAATTTTGAAAACTAAAAATAAGTAATAAAAGAATAAAAAAAGTAGC
TTTACTTCATAAAGTAGTGAAGAAGATGGTGATGATGATAGCTATTAAAATTGA>TTHERM_00684560(protein)
MQNNQQERLEFQEKYGIQKDYISLSKEDKKKQAADEVIEFNNKNLISYLEDKYQVEDTEK
VKEDKAKAIVKSCFMIQSGFNVCLHGYGSKRQIIQEISDQFINYPKLIIQGYSAATQIQQ
ILQKLKYIVVKITGQNSGQKRIEGILEYLKQQEENMGKISSQVPFILILFHNIDSKQFIE
QDSVKILSAVLNLPYIKAVCSIDHVKYPIILTSKVLESFNFSFIEVHTHQCYLHEFKVSQ
NTFNLKKEKEGESLKYIFRSLTKVQKEIIQFVAKYIQDKLDQNPGTAIQINGLTEDGQII
GLTLKELLEMLIDEMILNSEQQLKENLLEILDHKVIISIENQQKTYYTMNFSRQILEKIV
NNKLEDGEEDQEQEEEEENNSVKDMEEENQIISNSQNSSQSEDEDVKGKKKYRQTRREGK
KKKNSSESLSDSENEKHKFNKKTSGILKTKNKQQKNKKSSFTSQSSEEDGDDDSYQN>TTHERM_00684560(gene)
ATGCAAAATAATTAACAGGAAAGATTGGAATTCTAGGAAAAATATGGTATTTAAAAGGAC
TATATTTCTTTATCCAAAGAAGATAAAAAGAAATAAGCTGCAGATGAAGTCATTGAGTTT
AACAATAAAAACTTGATTTCTTATTTAGAAGATAAATATTAAGTAGAAGACACTGAAAAA
GTAAAAGAAGACAAAGCTAAAGCAATTGTGAAATCATGTTTTATGATACAATCAGGATTT
AATGTTTGCTTACATGGTTATGGCTCTAAGAGACAAATTATTTAAGAAATATCAGATTAA
TTTATTAATTACCCTAAATTAATCATTTAAGGATATTCAGCAGCTACTTAAATATAGCAA
ATTCTATAAAAACTAAAGTACATTGTGGTAAAGATTACTGGGTAAAACTCTGGTTAAAAG
AGAATTGAAGGAATATTAGAGTATCTAAAATAATAAGAGGAAAATATGGGAAAAATTTCC
TCTTAAGTACCTTTTATTTTAATTCTATTTCATAATATAGATTCTAAATAATTTATTGAG
TAAGATTCTGTCAAAATTTTATCTGCTGTACTAAATTTACCATATATAAAAGCTGTTTGT
AGTATTGATCATGTGAAGTATCCAATTATTCTGACTTCTAAAGTTTTAGAGTCATTTAAT
TTTAGTTTCATAGAAGTCCATACTCATTAATGCTATCTACATGAATTCAAAGTTTCCTAA
AATACATTTAACTTAAAAAAAGAAAAAGAAGGAGAATCTCTTAAATATATTTTTAGAAGT
CTGACAAAAGTTTAAAAAGAAATAATATAGTTTGTTGCAAAATATATTTAAGATAAATTA
GATTAAAATCCAGGAACAGCTATTTAAATAAATGGATTAACTGAAGATGGATAGATTATT
GGTTTAACTTTAAAAGAGTTATTAGAAATGCTAATTGATGAAATGATTTTGAACAGTGAG
TAGCAGTTAAAAGAAAATTTACTTGAAATACTTGATCACAAAGTTATAATTTCAATAGAA
AACTAGTAAAAAACTTACTATACAATGAATTTCAGCAGACAAATTTTAGAAAAAATTGTC
AACAACAAGTTGGAGGATGGAGAAGAAGATTAGGAGCAGGAAGAAGAGGAAGAAAACAAT
TCAGTAAAAGATATGGAAGAAGAGAATTAAATAATATCTAACAGCTAAAACAGTTCTCAA
AGTGAAGATGAAGATGTTAAAGGTAAAAAAAAATATAGATAAACAAGAAGAGAAGGTAAA
AAGAAAAAAAATAGTAGTGAGAGCTTATCAGATTCAGAAAATGAAAAACATAAGTTTAAT
AAGAAAACTTCTGGAATTTTGAAAACTAAAAATAAGTAATAAAAGAATAAAAAAAGTAGC
TTTACTTCATAAAGTAGTGAAGAAGATGGTGATGATGATAGCTATTAAAATTGA