Tian M, Mochizuki K, Loidl J (2019) Non-coding RNA Transcription in Tetrahymena Meiotic Nuclei Requires Dedicated Mediator Complex-Associated Proteins. Current biology : CB ( ): PUBMED:31280995
Garg J, Saettone A, Nabeel-Shah S, Cadorin M, Ponce M, Marquez S, Pu S, Greenblatt J, Lambert JP, Pearlman RE, Fillingham J (2019) The Med31 Conserved Component of the Divergent Mediator Complex in Tetrahymena thermophila Participates in Developmental Regulation. Current biology : CB ( ): PUBMED:31280994
Mutazono M, Noto T, Mochizuki K (2019) Diversification of small RNA amplification mechanisms for targeting transposon-related sequences in ciliates. Proceedings of the National Academy of Sciences of the United States of America ( ): PUBMED:31262823
Identifiers and Description
Gene Model Identifier
TTHERM_00865050
Standard Name
ORC1 (origin recognition complex subunit 1)
Aliases
PreTt29135 | 154.m00093 | 3751.m00439
Description
Largest subunit of the origin recognition complex
Genome Browser (Macronucleus)
Genome Browser (Micronucleus)
Gene Ontology Annotations
No Data fetched for Gene Ontology Annotations
Domains
No Data fetched for Domains
Gene Expression Profile
Tetrahymena Functional Genomics Database:
Change in Gene Expression
TMHMM
TMHMM
Signal Peptide
Signal Peptide Present?: No
Presense Probability: 10.1%
ORC1 Largest subunit of the or
igin recognition complex, whic
h directs DNA replication by b
inding to replication origins
and is also involved in transc
riptional silencing; exhibits
ATPase activity
Gene Information:
TTHERM_00865050 is a homolog of annotated and functionally characterized Orc1 in other experimental organisms (24% amino acid sequence identity, 49% similarity to human Orc1). Orc1 is a component of the heterohexameric origin recognition complex (ORC) found to be involved in initiation of DNA replication. TtOrc1 has canonical Walker A and B motifs (involved in ATP binding and hydrolysis). TtORC is unusual in that it contains an integral RNA subunit (26T RNA) that binds to its cognate DNA target in the ribosomal DNA (rDNA) replication origin.
Nucleic Acid Interactions (with the ORC complex):
26T RNA (5’-AUGUCUAAGUGUGAUGAUAAACGAAAAAAAAUAAAAAUUAA-3’).
Type I element T-rich strand: essential cis-acting replication determinant in ribosomal DNA origin of replication. (Location 5’ non-transcribed spacer)
(C3 rDNA type IB element T-rich strand, T51: 5’-CTCAAAAGTTGCAAAAGTTCGGAAGGTTTACTATTTTTGTTTTTTTTTT-3’). – requires 26T RNA and ATP
dsDNA – non rDNA chromosomes
Regulation & Expression:
mRNA and protein: Cell cycle regulated
Maximal protein expression at G1/S border, degraded in S phase
Associated Literature
Ref:19153611: Donti TR, Datta S, Sandoval PY, Kapler GM (2009) Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins. The EMBO journal 28(3):223-33
Ref:18007594: Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60
Ref:12832485: Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17
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Signal Peptide
Homologs (v.2006 protein sequences)
General Information 
Associated Literature 
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