Identifiers and Description

Gene Model Identifier

TTHERM_00463280

Standard Name

OLE1 (OLEic acid requiring)

Aliases

PreTt25780 | 54.m00236 | 3823.m01147

Description

Putative fatty acid desaturase; mRNA expression and stability increases upon decreased temperature; sequence similarity to delta-9-acyl-CoA desaturases from yeast, rat, and mouse

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Molecular Function

• oxidoreductase activity (IEA) | GO:0016491

Biological Process

• lipid metabolic process (IEA) | GO:0006629
• oxidation-reduction process (IEA) | GO:0055114

Domains

No Data fetched for Domains

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 9.6%

TetraMine Data

TTHERM_00463280

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_10724.1	9.997093137033517e-262	fatty acid desaturase (293 aa)
WormBase	WBGene00001398	9.99562814099401e-84	locus:fat-6 Delta(9)-fatty-a cid desaturase fat-6 status: Partially_confirmed UniProt: G5EGN2 protein_id:CAB08356.1
DictyBase	DDB_G0273837	5.001636743996964e-56	scdB-2 on chromosome: 2 positi on 3466919 to 3469700
SGD	YGL055W	5.0007856079333316e-54	OLE1 Delta(9) fatty acid desat urase, required for monounsatu rated fatty acid synthesis and for normal distribution of mi tochondria

General Information

No Data fetched for General Information

Associated Literature

1. Ref:10735176: Nakashima S, Nozawa Y (1999) Stress-responsive gene expression in Tetrahymena. Acta biologica Hungarica 50(4):413-24
2. Ref:8694777: Nakashima S, Zhao Y, Nozawa Y (1996) Molecular cloning of delta 9 fatty acid desaturase from the protozoan Tetrahymena thermophila and its mRNA expression during thermal membrane adaptation. The Biochemical journal 317 ( Pt 1)( ):29-34

Sequences

>TTHERM_00463280(coding)
ATGAACGTTAAAAATAAAATTAAATTAAAGTAAAATAAAATAAAAAAAATATACAAATAG
AATAAACTCACTCACACATACACACAAATGGGAGTATACTCTAAAGGTGAATTCAAGGTC
ATGGGTTATGAACTCAAGAAAGAGTATTGCATATGGTTAGGTTATATGCACATACTTGCT
GTAGCTGGTCTTATCTACTGGCTTTTATAAGAAAAGGAATTGTTCACAAAGAGTCTTTAC
ATGTTCCTTATTTTCCACTTGATGGGAGGTTTAGGTATTACTGCTGGCTCACATCGTCTC
TGGGCTCACAGAAGTTACACTGCCAAGACTCCCTACAGAATTTTGGTTATGCTCTTTAAC
AGCATGGCCTTCTAAGGTTCTATTTTCCACTGGAGCAGAGACCACAGACTCCACCATAAA
TTCTCGGACACAGATATTGATCCTCACTCAATGAACAAGGGATTCTTCTTTGCTCACGTT
GGCTGGTTACTTGTCAAGAAATCTCCTGAACTTAAAAAGGAAGGTGAAAAATTGAATATT
TAGGATCTTAAGGATGACCCTGTTGTTATGTTCCAAAAGAAGCACTACTTCTTGATTTCT
ACATTGGTCTGCTTTGTTATTCCAACTGTCATTATGCAATACACCATTGGAGGAGGATTC
ATCTACAACTTTTTGATGAGCTGTTTCAGATACGTCTTTATGCTTCACACTACCTGGTGT
GTAAACAGTGTATGCCACATGTTTGGTAGCAGACCTTATAACGAGTCTATTTTACCCACT
GAAAACCTCTTCGTTAGTATTATTGCTCTCGGTGAAGGATGGCACAACTGGCATCACGAA
TACCCCAGAGATTGGAGAGCTGCCGAAAATAAATGGACTAAGATCAACTTTACTTCCGGC
TTTATCAAGTTCTTCGAATTTTTGGGTCAATGTGATACCAATGGAATTAAGAAAAACGAA
TCTTGA

>TTHERM_00463280(protein)
MNVKNKIKLKQNKIKKIYKQNKLTHTYTQMGVYSKGEFKVMGYELKKEYCIWLGYMHILA
VAGLIYWLLQEKELFTKSLYMFLIFHLMGGLGITAGSHRLWAHRSYTAKTPYRILVMLFN
SMAFQGSIFHWSRDHRLHHKFSDTDIDPHSMNKGFFFAHVGWLLVKKSPELKKEGEKLNI
QDLKDDPVVMFQKKHYFLISTLVCFVIPTVIMQYTIGGGFIYNFLMSCFRYVFMLHTTWC
VNSVCHMFGSRPYNESILPTENLFVSIIALGEGWHNWHHEYPRDWRAAENKWTKINFTSG
FIKFFEFLGQCDTNGIKKNES

>TTHERM_00463280(gene)
ATGAACGTTAAAAATAAAATTAAATTAAAGTAAAATAAAATAAAAAAAATATACAAATAG
AATAAACTCACTCACACATACACACAAATGGGAGTATACTCTAAAGGTGAATTCAAGGTC
ATGGGTTATGAACTCAAGAAAGAGTATTGCATATGGTTAGGTTATATGCACATACTTGCT
GTAGCTGGTCTTATCTACTGGCTTTTATAAGAAAAGGAATTGTTCACAAAGAGTCTTTAC
ATGTTCCTTATTTTCCACTTGATGGGAGGTTTAGGTATTACTGCTGGCTCACATCGTCTC
TGGGCTCACAGAAGTTACACTGCCAAGACTCCCTACAGAATTTTGGTTATGCTCTTTAAC
AGCATGGCCTTCTAAGGTTCTATTTTCCACTGGAGCAGAGACCACAGACTCCACCATAAA
TTCTCGGACACAGATATTGATCCTCACTCAATGAACAAGGGATTCTTCTTTGCTCACGTT
GGCTGGTTACTTGTCAAGAAATCTCCTGAACTTAAAAAGGAAGGTGAAAAATTGAATATT
TAGGATCTTAAGGATGACCCTGTTGTTATGTTCCAAAAGAAGCACTACTTCTTGATTTCT
ACATTGGTCTGCTTTGTTATTCCAAGTAATTTTTTTTCTTTTATAAATACAAAAAAATTA
TAATAAATTTTGCCAATCGCGAAAACAAACAAACAAACAAACAATCTATAAATAAATTAT
CAATTTACTTAGTCATTTGCATAAATGGCTGTTAAGAAATTGAAACAATAAAAAGTGTTC
ATAAATCCAAATTTAAAATGAATTTTGATAAAAAAGCTTGTTAAAAAAAATCATTTTGAT
TCATTAATTAATTTATGCCAGTTTAAATATTCTAGAAAACTAATTTTTATTAAGCTAAAT
ATTCTAAACATCAATTTAATATTTAATAGCTGTCATTATGCAATACACCATTGGAGGAGG
ATTCATCTACAACTTTTTGATGAGCTGTTTCAGATACGTCTTTATGCTTCACACTACCTG
GTGTGTAAACAGTGTATGCCACATGTTTGGTAGCAGACCTTATAACGAGTCTATTTTACC
CACTGAAAACCTCTTCGTTAGTATTATTGCTCTCGGTGAAGGATGGCACAACTGGCATCA
CGAATACCCCAGAGATTGGAGAGCTGCCGAAAATAAATGGACTAAGATCAACTTTACTTC
CGGCTTTATCAAGTTCTTCGAATTTTTGGGTCAATGTGATACCAATGGAATTAAGAAAAA
CGAATCTTGA