Identifiers and Description

Gene Model Identifier

TTHERM_00467500

Standard Name

MBD1 (Fusion of methylthioribulose-phosphate dehydratase and dihydroxy-keto-methylthiopentene dioxygenase)

Aliases

PreTt18652 | 55.m00207 | 3710.m00056

Description

Fusion of two genes involved in the methionine salvage pathway; methylthioribulose-1-phosphate dehydratase -mtnB; and 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase - mtnD

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Molecular Function

• dioxygenase activity (IEA) | GO:0051213
• metal ion binding (IEA) | GO:0046872

Biological Process

• L-methionine salvage from methylthioadenosine (IEA) | GO:0019509
• oxidation-reduction process (IEA) | GO:0055114

Domains

• ( PF07883 ) Cupin domain
• ( PF03079 ) ARD/ARD' family
• ( TIGR01086 ) L-fuculose phosphate aldolase
• ( TIGR00760 ) L-ribulose-5-phosphate 4-epimerase
• ( TIGR03328 ) methylthioribulose-1-phosphate dehydratase
• ( PF00596 ) Class II Aldolase and Adducin

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 16.3%

TetraMine Data

TTHERM_00467500

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_15746.1	9.997093137033517e-262	ARD/ARD family protein (401 aa )
DictyBase	DDB_G0284915	4.99918933170902e-62	DDB_G0284915 on chromosome: 4 position 2643291 to 2644175
SGD	YJR024C	6.001950283815408e-48	MDE1 5-methylthioribulose-1-ph osphate dehydratase; acts in t he methionine salvage pathway; potential Smt3p sumoylation s ubstrate; expression downregul ated by caspofungin and deleti on mutant is caspofungin resis tant
WormBase	WBGene00013870	6.0018235484001396e-36	Probable methylthioribulose-1- phosphate dehydratase status :Confirmed UniProt:Q23261 protein_id:CAA88977.2

General Information

Paragraph No	Gene Name	Paragraph Text
18	MBD1	MBD1 is a gene fusion of two genes involved in the methionine salvage pathway: methylthioribulose-1-phosphate dehydratase -mtnB; and 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase - mtnD. These enzymes catalyze non-consecutive steps in the pathway. Interestingly the gene that codes for the intervening enzyme in the pathway, mtnC, is missing from the genome of Tetrahymena. Complementation tests in yeast were used to show that MBD1 from Tetrahymena is able to do in one step what yeast does in three, since it can rescue yeast knockouts of mtnB, mtnC, or mtnD (Salim, Negritto and Cavalcanti 2009).

Associated Literature

1. Ref:19851454: Salim HM, Negritto MC, Cavalcanti AR (2009) 1+1 = 3: a fusion of 2 enzymes in the methionine salvage pathway of Tetrahymena thermophila creates a trifunctional enzyme that catalyzes 3 steps in the pathway. PLoS genetics 5(10):e1000701

Sequences

>TTHERM_00467500(coding)
ATGAACGGTTTATCTTCAAATTCTGGTCCTAGAGAAGTCATTTGTGCTCTCTTGAGAAAC
TTTTATACTTAAGGATGGTGCGCTGGTTCAGGTGGTGGTATATCTATTAGAAAAAGCGAC
GATGAAATATATGTTGCACCTTCAGGTGTATAAAAAGAATTGGTTTAACCTGAAGATATC
TATGTTATTAATGTTAATGGAGATGTAGTTGAAAACCCTAAAAATCCAAAATTAAAACCT
TCTGAATGCACTCCTCTTTTTAATGCAGCTTATAAGCTTCGTCGTGCTGGTGCTGTTTTA
CATTCTCATGCTCTTCCTGCAATGCTAGTTACCAAGCTCTTTGGAACTGAATTTTAGACA
ATTGATCATGAAATGATTAAAGGTATTCCTAATCACCATAATACAGAATGGTGTGTCGTC
CCTATCATTGAAAACACTGAAAAGGAATGCGAATTAACAGAACGTTTAACTAATGCAATC
AACGCTTATCCTCGTTCTAATGCTGTCTTAGTTCGTAATCACGGTGTTTATATTTGGGGA
GAAAATTGGGAAAAAGCAAAAATACATGCTGAGTGTTACCATTATTTATTCGAAGCAGTT
GTTGAAATGAAGAAATTAGGTTTAGAAATTCCACGCACTGTTTCAAGCAGTAGCCAATTA
CGTGCTTGGTATATTGATGAAAATGCAATTGGCCAAGATGGTGATATTCGTGAGAGCTTA
CACTATCGCTCATATAAGTGGGTTAATCCAGAATATTTGGCAACAATTGGTGTTGAACAC
TGGAAATTAGACGGAGAAGAGAATAATACTGAACTTGAAAGAGTTTGCAAATAAAGAAAT
TACTCTTCTCGTGATTAAATTAAATGTGGAAATCATTGTGAAAATTACTAACAAATGTTA
TCAAATTTCAGAAAAGAACACATCCATTTAGATGAAGAGATTCGCTATATTATAGGTGGT
AGTGGTTATTTTGATGTCCGTGACCATGAAGATAAATGGATTCGTATCCAAAGTGTTAAA
GGTGATTTAATTGTACTTCCTGAAGGTATATATCACCGCTTTACAACTGATAAAAATGAT
GGAGTTCATGCTATGAGATTATTTAAGGATGAACCTAAATGGACACCTTATAATCGTCCT
TGTGATGAATTGGAAAGCCGTAACAAATATGTAGATTAATTCTTAAAAAAGTTTTAAAAA
TGA

>TTHERM_00467500(protein)
MNGLSSNSGPREVICALLRNFYTQGWCAGSGGGISIRKSDDEIYVAPSGVQKELVQPEDI
YVINVNGDVVENPKNPKLKPSECTPLFNAAYKLRRAGAVLHSHALPAMLVTKLFGTEFQT
IDHEMIKGIPNHHNTEWCVVPIIENTEKECELTERLTNAINAYPRSNAVLVRNHGVYIWG
ENWEKAKIHAECYHYLFEAVVEMKKLGLEIPRTVSSSSQLRAWYIDENAIGQDGDIRESL
HYRSYKWVNPEYLATIGVEHWKLDGEENNTELERVCKQRNYSSRDQIKCGNHCENYQQML
SNFRKEHIHLDEEIRYIIGGSGYFDVRDHEDKWIRIQSVKGDLIVLPEGIYHRFTTDKND
GVHAMRLFKDEPKWTPYNRPCDELESRNKYVDQFLKKFQK

>TTHERM_00467500(gene)
ATGAACGGTTTATCTTCAAATTCTGGTCCTAGAGAAGTCATTTGTGCTCTCTTGAGAAAC
TTTTATACTTAAGGATGGTGCGCTGGTTCAGGTGGTGGTATATCTATTAGAAAAAGCGAC
GATGAAATATATGTTGCACCTTCAGGTGTATAAAAAGAATTGGTTTAACCTGAAGATATC
TATGTTATTAATGTTAATGGAGATGTAGTTGAAAACCCTAAAAATCCAAAATTAAAACCT
TCTGAATGCACTCCTCTTTTTAATGCAGCTTATAAGCTTCGTCGTGCTGGTGCTGTTTTA
CATTCTCATGCTCTTCCTGCAATGCTAGTTACCAAGCTCTTTGGAACTGAATTTTAGACA
ATTGATCATGAAATGATTAAAGGTATTCCTAATCACCATAATACAGAATGGTGTGTCGTC
CCTATCATTGAAAACACTGAAAAGGAATGCGAATTAACAGAACGTTTAACTAATGCAATC
AACGCTTATCCTCGTTCTAATGCTGTCTTAGTTCGTAATCACGGTGTTTATATTTGGGGA
GAAAATTGGGAAAAAGCAAAAATACATGCTGAGTGTTACCATTATTTATTCGAAGCAGTT
GTTGAAATGAAGAAATTAGGTTTAGAAATTCCACGCACTGTTTCAAGCAGTAGCCAATTA
CGTGCTTGGTATATTGATGAAAATGCAATTGGCCAAGATGGTGATATTCGTGAGAGCTTA
CACTATCGCTCATATAAGTGGGTTAATCCAGAATATTTGGCAACAATTGGTGTTGAACAC
TGGAAATTAGACGGAGAAGAGAATAATACTGAACTTGAAAGAGTTTGCAAATAAAGAAAT
TACTCTTCTCGTGATTAAATTAAATGTGGAAATCATTGTGAAAATTACTAACAAATGTTA
TCAAATTTCAGAAAAGAACACATCCATTTAGATGAAGAGATTCGCTATATTATAGGTGGT
AGTGGTTATTTTGATGTCCGTGACCATGAAGATAAATGGATTCGTATCCAAAGTGTTAAA
GGTGATTTAATTGTACTTCCTGAAGGTATATATCACCGCTTTACAACTGATAAAAATGAT
GGAGTTCATGCTATGAGATTATTTAAGGATGAACCTAAATGGACACCTTATAATCGTCCT
TGTGATGAATTGGAAAGCCGTAACAAATATGTAGATTAATTCTTAAAAAAGTTTTAAAAA
TGA