Identifiers and Description

Gene Model Identifier

TTHERM_00633360

Standard Name

HTB1 (Histone h Two B )

Aliases

PreTt28005 | 90.m00169 | histone H2B-1

Description

HTB1 histone H2B.1; Histone H2B; one of the four histones (H2A- H2B- H3 and H4) that comprise the protein core of the eukaryotic nucleosome; differs from Htb2p by three amino acids

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

GBrowse

Gene Ontology Annotations

Cellular Component

Domains

  • ( PF00125 ) Core histone H2A/H2B/H3/H4
  • ( PF00808 ) Histone-like transcription factor (CBF/NF-Y) and

Gene Expression Profile

  • Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.8%

TetraMine Data

TTHERM_00633360

WebApollo

Fullscreen View

Tetrahymena Stock Center

  • ( SD01999 )
  • ( SD02031 ) Micronucleus: neo2 KO of HTB1 Macronucleus: KO of HTB1 with neo2
  • ( SD02078 ) Micronucleus: Neo2 into HTB1 replacing coding
  • ( SD02079 ) Micronucleus: Neo2 into HTB1 replacing coding
  • ( SD02080 ) Micronucleus: Neo2 into HTB1 replacing coding
  • ( SD02081 ) Micronucleus: Neo2 into HTB1 replacing coding
  • ( SD02082 ) Micronucleus: neo2 KO of HTB1 Macronucleus: KO of HTB1 with neo2
  • ( SD01882 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
  • ( SD01883 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with A8C mutation
  • ( SD01884 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with A8C, K4R mutation
  • ( SD01885 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with A8C, K4R mutation
  • ( SD02061 ) Micronucleus: neo2 replaces coding of HTB1,HTB2
  • ( SD02062 ) Micronucleus: neo2 replaces coding of HTB1,HTB2
  • ( SD02063 ) Micronucleus: neo2 replaces coding of HTB1,HTB2
  • ( SD02083 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
  • ( SD02084 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
  • ( SD02085 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
  • ( SD02086 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
  • ( SD02087 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: HTB1
  • ( SD02088 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: HTB1
  • ( SD02089 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
  • ( SD02090 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
  • ( SD02091 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
  • ( SD02093 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4R
  • ( SD02094 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4R
  • ( SD02095 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with S29A
  • ( SD02096 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with S29A
  • ( SD02097 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with S29A
  • ( SD02098 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A,T20A
  • ( SD02099 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A,T20A
  • ( SD02100 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A,T20A
  • ( SD02101 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T31A
  • ( SD02102 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T31A
  • ( SD02103 ) Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T31A
  • ( SD02104 ) Micronucleus: Neo2 into HTB2 replacing coding Macronucleus: HTB2 coding replaced by neo2 but assorted to pm-s
  • ( SD02105 ) Micronucleus: Neo2 into HTB2 replacing coding Macronucleus: HTB2 coding replaced by neo2 but assorted to pm-s
  • ( SD02466 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
  • ( SD02467 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
  • ( SD02468 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
  • ( SD02469 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
  • ( SD02470 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
  • ( SD02471 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
  • ( SD02472 ) Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2

Homologs (v.2006 protein sequences)

SourceIdentifierScoreDescription
Tetrahymena borealisEI9_15821.19.998074580622396e-81core histone H2A/H2B/H3/H4 fam
ily protein (122 aa)
OxytrichaContig678.1.g532.000065869309806e-55Core histone H2A/H2B/H3/H4
SGDYDR224C5.00115835552873e-49HTB1 Histone H2B, core histone
protein required for chromati
n assembly and chromosome func
tion; nearly identical to HTB2
; Rad6p-Bre1p-Lge1p mediated u
biquitination regulates transc
riptional activation, meiotic
DSB formation and H3 methylati
on
WormBaseWBGene000019156.998316056150599e-49locus:his-41 Probable histon
e H2B 3 status:Confirmed U
niProt:Q27484 protein_id:CAA
94740.1
DictyBaseDDB_G02865096.998020510507768e-25H2Bv3 on chromosome: 4 positio
n 4575107 to 4575571
Stentor CoeruleusSteCoe_21981.2995814250075031e-24

General Information

No Data fetched for General Information

Associated Literature

  1. Ref:30796450: Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J (2019) Proteomic analysis of histones H2A/H2B and variant Hv1 in Tetrahymena thermophila reveals an ancient network of chaperones. Molecular biology and evolution ( ):
  2. Ref:19822522: Wang Z, Cui B, Gorovsky MA (2009) Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. The Journal of biological chemistry 284(50):34870-9
  3. Ref:15199121: Medzihradszky KF, Zhang X, Chalkley RJ, Guan S, McFarland MA, Chalmers MJ, Marshall AG, Diaz RL, Allis CD, Burlingame AL (2004) Characterization of Tetrahymena histone H2B variants and posttranslational populations by electron capture dissociation (ECD) Fourier transform ion cyclotron mass spectrometry (FT-ICR MS). Molecular & cellular proteomics : MCP 3(9):872-86
  4. Ref:8760889: Liu X, Gorovsky MA (1996) Cloning and characterization of the major histone H2A genes completes the cloning and sequencing of known histone genes of Tetrahymena thermophila. Nucleic acids research 24(15):3023-30
  5. Ref:2713375: Nickel BE, Allis CD, Davie JR (1989) Ubiquitinated histone H2B is preferentially located in transcriptionally active chromatin. Biochemistry 28(3):958-63
  6. Ref:3039463: Nomoto M, Imai N, Saiga H, Matsui T, Mita T (1987) Characterization of two types of histone H2B genes from macronuclei of Tetrahymena thermophila. Nucleic acids research 15(14):5681-97
  7. Ref:6818230: Nomoto M, Kyogoku Y, Iwai K (1982) N-Trimethylalanine, a novel blocked N-terminal residue of Tetrahymena histone H2B. Journal of biochemistry 92(5):1675-8
  8. Ref:6804458: Nomoto M, Hayashi H, Iwai K (1982) Tetrahymena histone H2B. Complete amino acid sequence. Journal of biochemistry 91(3):897-904

Sequences 

>TTHERM_00633360(coding)
ATGGCTCCCAAGAAAGCTCCCGCTGCCGCTGCTGAAAAGAAGGTCAAGAAGGCCCCCACA
ACCGAAAAGAAGAACAAGAAGAAGAGATCAGAAACCTTCGCCATCTACATCTTCAAGGTC
TTAAAGCAAGTCCACCCTGATGTCGGTATTTCCAAGAAGGCTATGAACATTATGAACTCC
TTCATTAACGACTCCTTCGAAAGAATCGCCTTAGAATCCTCTAAGTTGGTCAGATTCAAC
AAGAGAAGAACCCTCTCATCCAGGGAAGTCTAAACCGCTGTCAAGCTCTTATTACCCGGT
GAACTCGCTAGACACGCCATCTCCGAAGGTACCAAGGCCGTCACCAAGTTCTCTTCTTCT
ACCAACTGA


>TTHERM_00633360(protein)
MAPKKAPAAAAEKKVKKAPTTEKKNKKKRSETFAIYIFKVLKQVHPDVGISKKAMNIMNS
FINDSFERIALESSKLVRFNKRRTLSSREVQTAVKLLLPGELARHAISEGTKAVTKFSSS
TN


>TTHERM_00633360(gene)
ATGGCTCCCAAGAAAGCTCCCGCTGCCGCTGCTGAAAAGAAGGTCAAGAAGGCCCCCACA
ACCGAAAAGAAGAACAAGAAGAAGAGATCAGAAACCTTCGCCATCTACATCTTCAAGGTC
TTAAAGCAAGTCCACCCTGATGTCGGTATTTCCAAGAAGGCTATGAACATTATGAACTCC
TTCATTAACGACTCCTTCGAAAGAATCGCCTTAGAATCCTCTAAGTTGGTCAGATTCAAC
AAGAGAAGAACCCTCTCATCCAGGGAAGTCTAAACCGCTGTCAAGCTCTTATTACCCGGT
GAACTCGCTAGACACGCCATCTCCGAAGGTACCAAGGCCGTCACCAAGTTCTCTTCTTCT
ACCAACTGA