Identifiers and Description

Gene Model Identifier

TTHERM_00433810

Standard Name

HEN1 (HEN1 (Hua ENhancer) homolog 1)

Aliases

PreTt26522 | 48.m00285 | 3831.m01622

Description

RNA methyltransferase; stabilizes scnRNA by 2'-O-methylation; ensures DNA elimination in Tetrahymena; colocalizes and interacts with TWI1; expressed during growth and early conjugation; homolog of Arabidopsis HEN1

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• macronucleus (IDA) | GO:0031039 | Ref:19240163

Molecular Function

• RNA methyltransferase activity (IDA) | GO:0008173 | Ref:19240163

Biological Process

• RNA catabolic process (IMP) | GO:0006401 | Ref:19240163
• programmed DNA elimination (IDA) | GO:0031049 | Ref:19240163
• snRNA 2'-O-methylation (IDA) | GO:1990437 | Ref:19240163

Domains

No Data fetched for Domains

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.2%

TetraMine Data

TTHERM_00433810

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_04218.1	9.997093137033517e-262	hypothetical protein (420 aa)
Stentor Coeruleus	SteCoe_13961	8.533047625744066e-17
Oxytricha	Contig5211.0.g50	0.00000000000020009182674645818	hypothetical protein
WormBase	WBGene00015349	0.000000009996807949091551	locus:henn-1 Uncharacterized protein C02F5.6 status:Part ially_confirmed UniProt:P342 83 protein_id:CCD62631.1

General Information

Paragraph No	Gene Name	Paragraph Text
109	HEN1, TWI1	Twi1p-associated scnRNAs are 2'-O-methylated at their 3' ends by the RNA methyltransferase Hen1p (Kurth & Mochizuki 2009).

Associated Literature

1. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
2. Ref:19240163: Kurth HM, Mochizuki K (2009) 2'-O-methylation stabilizes Piwi-associated small RNAs and ensures DNA elimination in Tetrahymena. RNA (New York, N.Y.) 15(4):675-85

Sequences

>TTHERM_00433810(coding)
ATGATTGAAGCTTATGAAACAGATGTGTTTATGGATCCTATTGGTATGAAAGTTTGGGAA
AAAAGGCACTAATATGTAGCAACTAAGTTATCGGCTCTGAATTGTAAAAGAGTTTTGGAT
ATGGGAACAAATACTTGCAAGCTCATATAAAGACTTTCAAGGAGTTTGTAATTCACTTAA
ATAGATGGTTTAGATATCGATGGCTAATTGCTTGAGACTCAAGGAATCCAAAATGCGAAG
CCAGATTTGATATAAAATTAATATGCCAGTATGAGAGACCATTAGCTAGTGGTAAATCTA
TACCAAGGCAGTGCTTTGAATAAGATTTAACATTTGAAGGATTAGCAATACGATGCTGTA
ATATTAGTTGAACTCATTGAGCACTTATAAGTAGAAGATGTTTTTCTTATTGAGTAAAAT
TTGTTTGGCTTTTTGAGGCCACAATTTGTGATTGTGACTACACCTAACTCTGATTTTAAT
GTTTATTTTAACTTTAAAGAATAAGGAGTATTGTTTAGGGACAAAGATCACAAATTTGAG
TGGTCCTAAAACTAGTTTTAGATTTGGGCCTAAAAAGTATGCTAAAATTATGGATACAAA
GTAATAGAGTTGACAGGAGTAGGCGAACATAAAACAGAAGGAACTAAGAACGGTTTTTGT
ACACAAATAGTTGTATTTGAGAAAGATACCCAACAAGAGAAGTATCTGAACTTTGCTTTC
TTCAACCTTCAAGAGGGAGAAATTCGTTAAGTATGTTAAATTCTGTACCCATTTGAGAGT
AAAGAATAACATTTTCAAAGAGAAGTAGTAGACAGTATCAGATATATTCTGCATATTACT
GATAAGTAAAATTAATTCGAAGATGGTTCATACTAAAATTATACTACTCTCTCTCGCATA
ATGCAAAATCATTCAATATCAAGTAATTGGTAAATTTAAGGTGATTACTTTAAACTTAAA
ACCTACATCCAGAATATTTCTGAGTTTTTGGTACATGAAAATTAGTTCAATTTTCAAGAA
TCTTTCGTCACTCTCAATTATTAAGCCTAAATGGAAGATGAAGAAAATGAAGATTAGCTT
GAAAGCGATTCTGAAAATGTAAAGATGCAATAATAACAATATTACTTTTCTAACGATAAT
TGTTTCTCCACAAAAGATACCACTTATAGCAGTTTTTCCACTGCAGATAACTTATTCTCC
TAAAAAATTTAACTTGGTTAGCAATAAATGGCTTTAGAGGAAATAGAATTAGAAGATACA
ATTGATTATTGA

>TTHERM_00433810(protein)
MIEAYETDVFMDPIGMKVWEKRHQYVATKLSALNCKRVLDMGTNTCKLIQRLSRSLQFTQ
IDGLDIDGQLLETQGIQNAKPDLIQNQYASMRDHQLVVNLYQGSALNKIQHLKDQQYDAV
ILVELIEHLQVEDVFLIEQNLFGFLRPQFVIVTTPNSDFNVYFNFKEQGVLFRDKDHKFE
WSQNQFQIWAQKVCQNYGYKVIELTGVGEHKTEGTKNGFCTQIVVFEKDTQQEKYLNFAF
FNLQEGEIRQVCQILYPFESKEQHFQREVVDSIRYILHITDKQNQFEDGSYQNYTTLSRI
MQNHSISSNWQIQGDYFKLKTYIQNISEFLVHENQFNFQESFVTLNYQAQMEDEENEDQL
ESDSENVKMQQQQYYFSNDNCFSTKDTTYSSFSTADNLFSQKIQLGQQQMALEEIELEDT
IDY

>TTHERM_00433810(gene)
AAAATAATTAAAAGTGCAAAAAAAAAAAATTAGAAATCTACCAAATAAACAAAAAGAAAG
AAAGAAAAAAATTTATTTATATTAAAAATGATTGAAGCTTATGAAACAGATGTGTTTATG
GATCCTATTGGTATGAAAGTTTGGGAAAAAAGGCACTAATATGTAGCAACTAAGTTATCG
GCTCTGAATTGTAAAAGAGTAAGATTAAAAACACATTTGATATTAAGATTGCTATTAACC
CTGATTTCATATTTTGTTAATAAATTAAGGTTTTGGATATGGGAACAAATACTTGCAAGC
TCATATAAAGACTTTCAAGGAGTTTGTAATTCACTTAAATAGTAAAATATCATTTTTCAA
TAATATTTGAATAGATTAATTAGTTTTATTCTTTAGGATGGTTTAGATATCGATGGCTAA
TTGCTTGAGACTCAAGGAATCCAAGTATAAATAATTTTAAATACGATTATAAAATAAATA
GTAAACATAAAACTTAGAATGCGAAGCCAGATTTGATATAAAATTAATATGCCAGTATGA
GAGACCATTAGCTAGTGGTAAATCTATACCAAGGCAGTGCTTTGAATAAGATTTAACATT
TGAAGGATTAGCAATACGATGCTGTAATATTAGTTGAACTCATTGAGCACTTATAAGTAG
AAGATGTTTTTCTTATTGAGTAAAATTTGTTTGGCTTTTTGAGGCCACAATTTGTGATTG
TGACTACACCTAACTCTGATTTTAATGTTTATTTTAACTTTAAGTAAAATCTTACAATTC
TAATAAAGGAAATTTAATGAATTATTTTTTATTTTCTAGAGAATAAGGAGTATTGTTTAG
GGACAAAGATCACAAATTTGAGTGGTCCTAAAACTAGTTTTAGATTTGGGCCTAAAAAGT
ATGCTAAAATTATGGTAAAACAAGAAGATAACTAAATGTTAAGTGAACTTATTATTTATT
TTTAATTTGTTTAGGATACAAAGTAATAGAGTTGACAGGAGTAGGCGAACATAAAACAGA
AGGAACTAAGAACGGTTTTTGTACACAAATAGTTGTATTTGAGAAAGATACCCAACAAGA
GAAGTATCTGAACTTTGCTTTCTTGTAAAAATTATTGATAAATAGTTTATTTAATTATTA
TTTTTTTAACTGAAAAAAGCAACCTTCAAGAGGGAGAAATTCGTTAAGTATGTTAAATTC
TGTACCCATTTGAGAGTAAAGAATAACATTTTCAAAGAGAAGTAGTAGACAGTATCAGGT
ATTTAATTTATTAAGAAATAGATTTTAAATACTTTTTTGAGACTAAATATTTTTTATTTC
TAATTAGATATATTCTGCATATTACTGATAAGTAAAATTAATTCGAAGATGGTTCATACT
AAAATTATACTACTCTCTCTCGCATAATGCAAAATCATTCAATATCAAGTAATTGGTAAA
TTTAAGGTGATTACTTTAAACTTAAAACCTACATCCAGAATATTTCTGAGTTTTTGGTAC
ATGAAAATTAGTTCAATTTTCAAGAATCTTTCGTCACTCTCAATTATTAAGCCTAAATGG
AAGATGAAGAAAATGAAGATTAGCTTGAAAGCGATTCTGAAAATGTAAAGATGCAATAAT
AACAATATTACTTTTCTAACGATAATTGTTTCTCCACAAAAGATACCACTTATAGCAGTT
TTTCCACTGCAGATAACTTATTCTCCTAAAAAATTTAACTTGGTTAGCAATAAATGGCTT
TAGAGGAAATAGAATTAGAAGATACAATTGATTATTGA