Tian M, Loidl J (2018) A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation. Nucleic acids research ( ): PUBMED:30357385
Ali EI, Loidl J, Howard-Till RA (2018) A streamlined cohesin apparatus is sufficient for mitosis and meiosis in the protist Tetrahymena. Chromosoma ( ): PUBMED:29948142
Gamma-tubulin; GTU1p is essential; localizes to microtubule-organizing centers in vegetative cells: basal bodies, macronuclear envelopes, micronuclear envelopes, and contractile vacuole
( SD02293 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD01414 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
( SD01215 ) Macronucleus: GTU1 gene completely replaced by GTU1 gene with HA tag at the C terminus. Also has neo2 cassette in the 3' flanking region
( SD01340 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
( SD01341 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
( SD01342 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
( SD01363 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
( SD01364 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
( SD01365 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
( SD01366 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
( SD01367 ) Micronucleus: Neo2 replaces coding of GTU1
( SD01368 ) Micronucleus: Neo2 replaces coding of GTU1
( SD01369 ) Micronucleus: Neo2 replaces coding of GTU1
( SD01412 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
( SD01413 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
( SD01415 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
( SD01416 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
( SD01417 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal GFP tag into MTT1 locus, partial replacement
( SD02043 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations A101G and c-terminus HA tag into the MTT1 locus, partial replacement.
( SD02044 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02045 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02047 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
( SD02048 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
( SD02051 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124K and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02052 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124K into the MTT1 locus, partial replacement.
( SD02057 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A into the MTT1 locus, partial replacement.
( SD02058 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A and C-terminal HA tag into the MTT1 locus, partial replacement.
( SD02143 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
( SD02144 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
( SD02145 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
( SD02146 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
( SD02147 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
( SD02192 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A,Y272A. Not sure if complete replacement.
( SD02193 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A,Y272A. Not sure if complete replacement.
( SD02194 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
( SD02195 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
( SD02210 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation D116A,D117A and c-terminus HA tag into the MTT1 locus, partial replacement.
( SD02211 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation D116A,D117A and c-terminus HA tag into the MTT1 locus, partial replacement.
( SD02292 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02306 ) Macronucleus: GTU1 with c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
( SD02307 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
( SD02308 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations K358A,K359A anc c-terminus HA tag into the MTT1 locus, partial replacement.
( SD02473 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02474 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02516 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation R285D,K286D and HA tag at c-teminus into the MTT1 locus, partial replacement.
( SD02531 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
locus:tbg-1 Tubulin gamma ch
ain status:Confirmed UniPr
ot:P34475 protein_id:CAA8016
4.1
General Information
No Data fetched for General Information
Associated Literature
Ref:25660693: Kushida Y, Takaine M, Nakano K, Sugai T, Numata O (2015) Dynamic Change of Cellular Localization of Microtubule-Organizing Center During Conjugation of Ciliate Tetrahymena thermophila. Zoological science 32(1):25-32
Ref:21246753: Kushida Y, Nakano K, Numata O (2011) Amitosis requires ?-tubulin-mediated microtubule assembly in Tetrahymena thermophila. Cytoskeleton (Hoboken, N.J.) 68(2):89-96
Ref:16344310: Shang Y, Tsao CC, Gorovsky MA (2005) Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. The Journal of cell biology 171(6):1035-44
Ref:11891286: Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 99(6):3734-9
Ref:12356864: Shang Y, Li B, Gorovsky MA (2002) Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. The Journal of cell biology 158(7):1195-206
Ref:8864661: Liang A, Ruiz F, Heckmann K, Klotz C, Tollon Y, Beisson J, Wright M (1996) Gamma-tubulin is permanently associated with basal bodies in ciliates. European journal of cell biology 70(4):331-8
Ref:8124706: Stearns T, Kirschner M (1994) In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin. Cell 76(4):623-37