Identifiers and Description

Gene Model Identifier

TTHERM_00079520

Standard Name

GTU1 (Gamma-TUbulin 1)

Aliases

PreTt10197 | 5.m00548 | 3828.m01461

Description

Gamma-tubulin; GTU1p is essential; localizes to microtubule-organizing centers in vegetative cells: basal bodies, macronuclear envelopes, micronuclear envelopes, and contractile vacuole

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• contractile vacuole pore (IDA) | GO:0031913 | Ref:12356864
• protein complex (IEA) | GO:0043234
• microtubule basal body (IDA) | GO:0005932 | Ref:12356864
• gamma-tubulin complex (ISS) | GO:0000930 | Ref:12356864
• micronucleus (IDA) | GO:0031040 | Ref:12356864
• oral apparatus (IDA) | GO:0031912 | Ref:8124706
• macronucleus (IDA) | GO:0031039 | Ref:12356864
• nuclear envelope (IDA) | GO:0005635 | Ref:12356864

Molecular Function

• structural constituent of cytoskeleton (ISS) | GO:0005200 | Ref:12356864
• GTP binding (IEA) | GO:0005525
• GTPase activity (IEA) | GO:0003924

Biological Process

• ciliary basal body duplication (IMP) | GO:0032054 | Ref:12356864
• microtubule nucleation (ISS) | GO:0007020 | Ref:12356864
• protein polymerization (IEA) | GO:0051258

Domains

• ( PF00091 ) Tubulin/FtsZ family, GTPase domain
• ( PF03953 ) tubulin/FtsZ family, C-terminal domain

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.9%

TetraMine Data

TTHERM_00079520

WebApollo

Fullscreen View

Tetrahymena Stock Center

• ( SD02293 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD01414 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
• ( SD01215 ) Macronucleus: GTU1 gene completely replaced by GTU1 gene with HA tag at the C terminus. Also has neo2 cassette in the 3' flanking region
• ( SD01340 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
• ( SD01341 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
• ( SD01342 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
• ( SD01363 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD01364 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD01365 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD01366 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD01367 ) Micronucleus: Neo2 replaces coding of GTU1
• ( SD01368 ) Micronucleus: Neo2 replaces coding of GTU1
• ( SD01369 ) Micronucleus: Neo2 replaces coding of GTU1
• ( SD01412 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
• ( SD01413 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
• ( SD01415 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
• ( SD01416 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
• ( SD01417 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal GFP tag into MTT1 locus, partial replacement
• ( SD02043 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations A101G and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02044 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02045 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02047 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
• ( SD02048 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
• ( SD02051 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124K and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02052 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124K into the MTT1 locus, partial replacement.
• ( SD02057 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A into the MTT1 locus, partial replacement.
• ( SD02058 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A and C-terminal HA tag into the MTT1 locus, partial replacement.
• ( SD02143 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02144 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02145 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02146 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02147 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02192 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A,Y272A. Not sure if complete replacement.
• ( SD02193 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A,Y272A. Not sure if complete replacement.
• ( SD02194 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02195 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02210 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation D116A,D117A and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02211 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation D116A,D117A and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02292 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02306 ) Macronucleus: GTU1 with c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD02307 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD02308 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations K358A,K359A anc c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02473 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02474 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02516 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation R285D,K286D and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02531 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Stentor Coeruleus	SteCoe_21979	0
Tetrahymena borealis	EI9_06993.1	9.997093137033517e-262	tubulin/FtsZ family, GTPase do main-containing protein (450 a a)
DictyBase	DDB_G0271738	9.997093137033517e-262	tubC on chromosome: 2 position 727031 to 728611
Oxytricha	Contig3513.0.g10	9.997093137033517e-262	Tubulin/FtsZ family, GTPase do main
SGD	YLR212C	9.996050283847121e-108	TUB4 Gamma-tubulin, involved i n nucleating microtubules from both the cytoplasmic and nucl ear faces of the spindle pole body
WormBase	WBGene00006540	4.001242549923285e-107	locus:tbg-1 Tubulin gamma ch ain status:Confirmed UniPr ot:P34475 protein_id:CAA8016 4.1

General Information

No Data fetched for General Information

Associated Literature

1. Ref:25660693: Kushida Y, Takaine M, Nakano K, Sugai T, Numata O (2015) Dynamic Change of Cellular Localization of Microtubule-Organizing Center During Conjugation of Ciliate Tetrahymena thermophila. Zoological science 32(1):25-32
2. Ref:21246753: Kushida Y, Nakano K, Numata O (2011) Amitosis requires ?-tubulin-mediated microtubule assembly in Tetrahymena thermophila. Cytoskeleton (Hoboken, N.J.) 68(2):89-96
3. Ref:16344310: Shang Y, Tsao CC, Gorovsky MA (2005) Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. The Journal of cell biology 171(6):1035-44
4. Ref:11891286: Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 99(6):3734-9
5. Ref:12356864: Shang Y, Li B, Gorovsky MA (2002) Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. The Journal of cell biology 158(7):1195-206
6. Ref:8864661: Liang A, Ruiz F, Heckmann K, Klotz C, Tollon Y, Beisson J, Wright M (1996) Gamma-tubulin is permanently associated with basal bodies in ciliates. European journal of cell biology 70(4):331-8
7. Ref:8124706: Stearns T, Kirschner M (1994) In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin. Cell 76(4):623-37

Sequences

>TTHERM_00079520(coding)
ATGCCTAGAGATATAATTACACTTTAAGTCGGCCAATGCGGAAACTAAATTGGTATGGAA
TTTTGGAAATAGCTTTGTTTAGAGCATGGTATTAACCAAGAAGGTATTTTACAAGAATAT
GCAACAAACACTGATGACAGAAAAGAAGTTTTCTTTTATTAAGCAGATGATTAGCATTAT
ATTCCAAGGGCCTGTCTTATTGATTTAGAAGACAGAGTTCTAAAGAGAATAGTTAAATAA
TCTGATTATAGTTCACTATATAACCCAGAAAATATTTTTTATGGAACTGATGGATCAGGT
GCTGGTAATGTGTGGGCATACGGTTATTAAGAAGCTAATAAAATCCAAGATGATCTTCTT
GATATGATTGACAGAGAAGCTGATACATCTGATAGTTTTGAAGCTTTTCTTTTGATTCAT
AGTATTGCAGGTGGTACTGGTTCAGGTGTTGGCTCTTATTTATTGGAGAAATTAAATGAT
AGATTCCCTAAAAAAATTATTAAGACTTACTCTGTTTTTCCTAGTGGACACACAGATGGT
GTCGTACATCCTTATAACTCTCTTTTAACTTTAAAGAGACTTGCTTTGAATGCAGATGCA
ACCACTGTTTTAGATAATAACGCTTTAGAAAAAATAGCACAAGACAGATTAAAATTGGAA
AATCCTACAATTGAAACCACAAACTCACTTGTCTCAACAGTTATGGCAGCATGTACTACC
ACACTCAGATACCCTGGTTATATGAATAACGATTTAGTTGGTTTACTTGCATCTCTTGTT
CCTACACCTAAATGTCATTTCTTAATGACTGGATATACTCCTATCTCTATTGATAGACAT
ATAAAAACTATTAGAAAGACTACTGTACTTGATGTTATGAGAAGATTACTTTAAACAAAG
AATATCATGGTTAGTGCTTCAACTAAAAAGGGACAATATATTTCAATTATGAATGTAATT
CAAGGAGAAGTTGATGCATCTCAAATTCATAAATCACTTTAGCGTATTAGAGAAAGAAAA
TTAGCTAACTTTATTAACTGGGGACCTGCCTCCATACAAGTTGCATTATCTAAAAAATCA
CCATACATAGAAACATCTCATAAAGTAAGTGGTCTTATGCTCGCCAACCACACAAGCATC
CATACACTTTTTGAGAAGATTATCTCTAACTTTGACATAATGAGAAAAAAGAATGCTTAT
ATAGAATAGTTTAAAAAGGTTGATTCTTTTAACTTAGAGGAGTTCGATGATTCCAAGGAA
GTAGTACAATAATTAATAGAAGAATATAAAGCTGCTGAAGAACCTAACTATTTAGATTAC
TTCGATGATGGCTTGGAAGACGAAAACTGA

>TTHERM_00079520(protein)
MPRDIITLQVGQCGNQIGMEFWKQLCLEHGINQEGILQEYATNTDDRKEVFFYQADDQHY
IPRACLIDLEDRVLKRIVKQSDYSSLYNPENIFYGTDGSGAGNVWAYGYQEANKIQDDLL
DMIDREADTSDSFEAFLLIHSIAGGTGSGVGSYLLEKLNDRFPKKIIKTYSVFPSGHTDG
VVHPYNSLLTLKRLALNADATTVLDNNALEKIAQDRLKLENPTIETTNSLVSTVMAACTT
TLRYPGYMNNDLVGLLASLVPTPKCHFLMTGYTPISIDRHIKTIRKTTVLDVMRRLLQTK
NIMVSASTKKGQYISIMNVIQGEVDASQIHKSLQRIRERKLANFINWGPASIQVALSKKS
PYIETSHKVSGLMLANHTSIHTLFEKIISNFDIMRKKNAYIEQFKKVDSFNLEEFDDSKE
VVQQLIEEYKAAEEPNYLDYFDDGLEDEN

>TTHERM_00079520(gene)
ATGCCTAGAGATATAATTACACTTTAAGTCGGCCAATGCGGAAACTAAAGTATTAGATTT
ATTTGAGATTAGAGTTTAAATAATGGAGATTTGCTTATTATTTTAGCACTTCATCAGCTT
TATAGGAATGGATGGATTTATAATTTAATTTTTTTAAAAATTCAAGTTGGTATGGAATTT
TGGAAATAGCTTTGTTTAGAGCATGGTATTAACCAAGAAGGTATTTTACAAGAATATGCA
ACAAACACTGATGACAGAAAAGAAGTTTTCTTTTATTAAGCAGATGATTAGCATTATATT
CCAAGGGCCTGTCTTATTGATTTAGAAGACAGAGTTCTAAAGAGAATAGTTAAATAATCT
GATTATAGTTCACTATATAACCCAGAAAATATTTTTTATGGAACTGATGGATCAGGTGCT
GGTAATGTGTGGGCATACGGTTATTAAGAAGCTAATAAAAGTAAATTTTAAAACTTATAA
ATTAAAAATTGTTATAAAATTACTAAAAATGTTGTGTTTTAGATAAGCAAAATTTTTAAA
ATTATATCAAAATTTATTGAAAGAATTAAGGAAATTCGATTTAAAGATTGAGATTAAATG
AAAGAATTGCATAGAAATATAATGGATTTTAATCTATAATTTACAAATAAAAATAATTGA
GCTTATCAAATTTTTAAAAAAATTATGTTCTCTCATTTATATGTGATGCTTTATTATAAT
TTAAAAAATATTAGTCCAAGATGATCTTCTTGATATGATTGACAGAGAAGCTGATACATC
TGATAGTTTTGAAGCTTTTCTTTTGATTCATAGTATTGCAGGTGGTACTGGTTCAGGTGT
TGGCTCTTATTTATTGGAGAAATTAAATGATAGATTCCCTAAAAAAATTATTAAGACTTA
CTCTGTTTTTCCTAGTGGACACACAGATGGTGTCGTACATCCTTATAACTCTCTTTTAAC
TTTAAAGAGACTTGCTTTGAATGCAGATGCAACCACTGTTTTAGATAATAACGCTTTAGA
AAAAATAGCACAAGACAGATTAAAATTGGAAAATCCTACAATTGAAACCACAAACTCACT
TGTCTCAACAGTTATGGCAGCATGTACTACCACACTCAGATACCCTGGTTATATGAATAA
CGATTTAGTTGGTTTACTTGCATCTCTTGTTCCTACACCTAAATGTCATTTCTTAATGAC
TGGATATACTCCTATCTCTATTGATAGACATATAAAAACTATTAGAAAGACTACTGTACT
TGATGTTATGAGAAGATTACTTTAAACAAAGAATATCATGGTTAGTGCTTCAACTAAAAA
GGGACAATATATTTCAATTATGAATGTAATTCAAGGAGAAGTTGATGCATCTCAAATTCA
TAAATCACTTTAGCGTATTAGAGAAAGAAAATTAGGTAAAATTTTAAATAAATAAAAATT
GAATGAATAAAAATATTTTAAAAAAAGCTAACTTTATTAACTGGGGACCTGCCTCCATAC
AAGTTGCATTATCTAAAAAATCACCATACATAGAAACATCTCATAAAGTAAGTGGTCTTA
TGCTCGCCAACCACACAAGCATCCATACAGTAATAATTATTATTATTATTCTAAAATTAA
AAAAATATTTTTTGCCTTATATTAAAAGCTTTTTGAGAAGATTATCTCTAACTTTGACAT
AATGAGAAAAAAGAATGCTTATATAGAATAGTTTAAAAAGGTTGATTCTTTTAACTTAGA
GGAGTTCGATGATTCCAAGTAAATAAACTCTAAAAATCATTTTTAATTAATATATTTATT
ACTAATTTAAATAGGGAAGTAGTACAATAATTAATAGAAGAATATAAAGCTGCTGAAGAA
CCTAACTATTTAGATTACTTCGATGATGGCTTGGAAGACGAAAACTGA