Identifiers and Description

Gene Model Identifier

TTHERM_01194720

Standard Name

DES5 (C-5(6) sterol DESaturase 5 )

Aliases

PreTt07509 | 270.m00035

Description

Catalyzes the introduction of a C-5(6) double bond into lathosterol and cholestanol

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• endoplasmic reticulum (IEA) | GO:0005783

Molecular Function

• C-5 sterol desaturase activity (IDA) | GO:0000248 | Ref:19525418
• oxidoreductase activity (IEA) | GO:0016491
• iron ion binding (IEA) | GO:0005506

Biological Process

• oxidation-reduction process (IEA) | GO:0055114
• fatty acid biosynthetic process (IEA) | GO:0006633

Domains

• ( PF01598 ) Sterol desaturase

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 14.4%

TetraMine Data

TTHERM_01194720

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_21011.1	9.997093137033517e-262	sterol desaturase (335 aa)
Stentor Coeruleus	SteCoe_10647	2.2856936767186716e-49
SGD	YLR056W	5.9971655715910366e-21	ERG3 C-5 sterol desaturase, ca talyzes the introduction of a C-5(6) double bond into episte rol, a precursor in ergosterol biosynthesis; mutants are via ble, but cannot grow on non-fe rmentable carbon sources
Oxytricha	Contig18492.0.g71	0.000000030002053728821195	Fatty acid hydroxylase superfa mily
DictyBase	DDB_G0279611	0.00010003404299092957	DDB_G0279611 on chromosome: 3 position 2316340 to 2317367

General Information

No Data fetched for General Information

Associated Literature

1. Ref:19525418: Nusblat AD, Najle SR, Tomazic ML, Uttaro AD, Nudel CB (2009) C-5(6) sterol desaturase from tetrahymena thermophila: Gene identification and knockout, sequence analysis, and comparison to other C-5(6) sterol desaturases. Eukaryotic cell 8(8):1287-97

Sequences

>TTHERM_01194720(coding)
ATGTTACAATTATAATTTTAAAATTCTTATTTAATTTATTGTTTGTTTTTGGGAAAGTAT
AATAAATAATTTACTATATTAATCGTGCTAAATGGATTAAAGATACATACATAAATAAAT
ATCAATCTTTAATAAAAATAAATAACAATAGCTTAAAGGCTAAATCAAGTTGAATAAATT
CAAGAAAAAAAATAAATTAAGTCCTCAATGGTTTATTGGCTTATTGCTGAATAGTTAGGT
TATTTTAATGTAGATAGAGTTAAGGTGATGCAAGATGCCAAGGATCACATTAGCTCTTCA
CTCAGATTGATTTAATTAGATAAATTATTCCCTACTTTCTCAAATTTTATTCTAGATTCT
GCTGGTTAATAAATTGGTAACATAATTATAGAATATATTTGCACTCTGATTCATACTATA
GTTTTCGCTTACATCCTCTACTTCACTGCAGCTACATCCTCCTACTTTTATTTCTTTGTG
TACAAGAAAAAGAAATATTTGCCTGAATTTAAAGGAGATTTAAAAATTGCATTTGATATC
AAATGGTCTTGTATTAACATTTTTGTTGAATCATTTTTAGTATCTGCATTAAGATTAGCT
ATGCCCAGATTCTCTATGATCTACTATGATATCAATGATCATAGTTGGCTCTACATCCCT
TTTAGCATTTTATTTCATATGATGTTTGATGAAACCCTAACTTACTGGGTTCATAGATGG
CTCCACACCTTCCCTTATTTATATACCAAATTGCATGTAGTTCATCATAGATCAAAAGAT
GTTACACCCTTCTCTGGCTTTGCTTTCCACCCACTTGATGCTTTTGCCTAAGCTGTCCCC
ACTTTTGTAAGCTGTTATTTCTTCCCTCTCCATATTAACATCTTGCTTGGATTCTCATTA
ATTACCACAATCTGGGCCATTTCTATTCACGATAATGTTCCTCTAGTGCCTTGCAAGCTC
TTTTTGTACAGTACCCACCATTCTATCCACCACGAAGCTGGAAGAGGTTAAATGACTAAC
TATGGTAAATTTACTTCTGTATGGGACAGAATATGTTAAACTTACTGGGATCCTGACAGG
GTTTATTTTGGATGGGATGAATGGAAATAAAGAAAAGGAATATTCAGTAAATTCAATAGC
TTTTATAGCAAAATAGTACCTGATAAAAGCTCAGAAGAACTAAACAAATAAATTAAACAA
AAAAAGAATTGA

>TTHERM_01194720(protein)
MLQLQFQNSYLIYCLFLGKYNKQFTILIVLNGLKIHTQININLQQKQITIAQRLNQVEQI
QEKKQIKSSMVYWLIAEQLGYFNVDRVKVMQDAKDHISSSLRLIQLDKLFPTFSNFILDS
AGQQIGNIIIEYICTLIHTIVFAYILYFTAATSSYFYFFVYKKKKYLPEFKGDLKIAFDI
KWSCINIFVESFLVSALRLAMPRFSMIYYDINDHSWLYIPFSILFHMMFDETLTYWVHRW
LHTFPYLYTKLHVVHHRSKDVTPFSGFAFHPLDAFAQAVPTFVSCYFFPLHINILLGFSL
ITTIWAISIHDNVPLVPCKLFLYSTHHSIHHEAGRGQMTNYGKFTSVWDRICQTYWDPDR
VYFGWDEWKQRKGIFSKFNSFYSKIVPDKSSEELNKQIKQKKN

>TTHERM_01194720(gene)
ATGTTACAATTATAATTTTAAAATTCTTATTTAATTTATTGTTTGTTTTTGGGAAAGTAT
AATAAATAATTTACTATATTAATCGTGCTAAATGGATTAAAGATACATACATAAATAAAT
ATCAATCTTTAATAAAAATAAATAACAATAGCTTAAAGGCTAAATCAAGTTGAATAAATT
CAAGAAAAGTAAAACAAGATTTATATATAATATTATTATTTAAAATAGAACTATCATAAA
ATATTATTAGAAAATAAATTAAGTCCTCAATGGTTTATTGGCTTATTGCTGAATAGTTAG
GTTATTTTAATGTAGATAGAGTTAAGGTGATGCAAGATGCCAAGGATCACATTAGCTCTT
CACTCAGATTGATTTAATTAGATAAATTATTCCCTACTTTCTCAAATTTTATTCTAGATT
CTGCTGGTTAATAAATTGGTAACATAATTATAGAATATATTTGCACTCTGATTCATACTA
TAGTTTTCGCTTACATCCTCTACTTCACTGCAGCTACATCCTCCTACTTTTATTTCTTTG
TGTAAATTTTTTTAAAAAGTAATTAAGCAAATAAGAGTTAAAAACTTCATAAATAGGTAC
AAGAAAAAGAAATATTTGCCTGAATTTAAAGGAGATTTAAAAATTGCATTTGATATCAAA
TGGTCTTGTATTAACATTTTTGTTGAATCATTTTTAGTATCTGCATTAAGATTAGCTATG
CCCAGATTCTCTATGATCTACTATGATATCAATGATCATAGTTGGCTCTACATCCCTTTT
AGCATTTTATTTCATATGATGTTTGATGAAACCCTAACTTACTGGGTTCATAGATGGCTC
CACACCTTCCCTTATTTATATACCAAAGTATTTTCTTTTAACTCTTAATCATTTTAACAA
ACTGATTAGCTTTTTTAAAGCATTAACAAGATAACTACTTTATAGAATTAGATTTAATTA
GATATTTTATTACCAATAGAAATTAAATAGATCTAACTATCTATCAATTGATATCTCAAT
ATTTAATTATTTTTTTGCAAATCTGATGAATTCAAATTTTAATAATATAATTAAATGTTT
TTTTAATTAATTACAAATATCTTTTGTATTAATTTCATAATTTATAATTAGTTGCATGTA
GTTCATCATAGATCAAAAGATGTTACACCCTTCTCTGGCTTTGCTTTCCACCCACTTGAT
GCTTTTGCCTAAGCTGTCCCCACTTTTGTAAGCTGTTATTTCTTCCCTCTCCATATTAAC
ATCTTGCTTGGATTCTCATTAATTACCACAATCTGGGCCATTTCTATTCACGATAATGTT
CCTCTAGTGCCTTGCAAGCTCTTTTTGTACAGTACCCACCATTCTATCCACCACGAAGCT
GGAAGAGGTTAAATGACTAACTATGGTAAATTTACTTCTGTATGGGACAGAATATGTTAA
ACTTACTGGGATCCTGACAGGGTTTATTTTGGATGGGATGAATGGAAATAAAGAAAAGGA
ATATTCAGTAAATTCAATAGCTTTTATAGCAAAATAGTACCTGATAAAAGCTCAGAAGAA
CTAAACAAATAAATTAAACAAAAAAAGAATTGA