Zhao X, Xiong J, Mao F, Sheng Y, Chen X, Feng L, Dui W, Yang W, Kapusta A, Feschotte C, Coyne RS, Miao W, Gao S, Liu Y (2019) RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena. Genes & development 33(5-6):348-364 PUBMED:30808657
Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J (2019) Proteomic analysis of histones H2A/H2B and variant Hv1 in Tetrahymena thermophila reveals an ancient network of chaperones. Molecular biology and evolution ( ): PUBMED:30796450
Tian M, Loidl J (2018) A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation. Nucleic acids research ( ): PUBMED:30357385
Identifiers and Description
Gene Model Identifier
TTHERM_00881440
Standard Name
CYP1 (CYsteine Protease 1
)
Aliases
PreTt09744 | 156.m00128 | CyP
Description
Putative cysteine protease; mRNA is specifically expressed during starvation; histone H1 and Hhp1p are required for efficient mRNA expression
HHO1 knockouts show no global increase or decrease in the amount of transcription in the cell; however, these same knockouts also show that Hho1p is important for the transcriptional regulation of individual genes in response to stimuli, such as starvation. The differential regulation of Hho1p by phosphorylation under vegetative growth and starvation conditions has been well studied. During vegetative growth, Hho1p is phosphorylated on five closely spaced residues, preventing it from interacting with chromatin, likely by interfering with its ability to bind DNA. Under these conditions, expression is increased for CDC2, a homolog of the cyclin dependent kinases responsible for histone H1 phosphorylation, possibly creating a positive feedback loop that promotes the cell cycle. During starvation conditions, Hho1p is dephosphorylated, allowing it to bind to chromatin. This stimulates the expression of some genes, including ngoA, and protease genes such as CYP1, while inhibiting expression of other genes, such as CDC2. This decrease in CDC2 expression may be responsible for cell cycle arrest during starvation.
Associated Literature
Ref:12356861: Dou Y, Bowen J, Liu Y, Gorovsky MA (2002) Phosphorylation and an ATP-dependent process increase the dynamic exchange of H1 in chromatin. The Journal of cell biology 158(7):1161-70
Ref:12351190: Udani RA, Karrer KM (2002) ASI1, a gene encoding a novel leucine zipper protein, is induced during development of the macronucleus in Tetrahymena. Mechanisms of development 118(1-2):215-8
Ref:10983971: Dou Y, Gorovsky MA (2000) Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Molecular cell 6(2):225-31
Ref:10207086: Huang H, Smothers JF, Wiley EA, Allis CD (1999) A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila. Molecular and cellular biology 19(5):3624-34
Ref:9163512: Suzuki KM, Hosoya N, Takahashi T, Kosaka T, Hosoya H (1997) Release of a newly-identified cysteine protease, tetrain, from Tetrahymena into culture medium during the cell growth. Journal of biochemistry 121(4):642-7
Ref:8756729: Shen X, Gorovsky MA (1996) Linker histone H1 regulates specific gene expression but not global transcription in vivo. Cell 86(3):475-83
Ref:8464925: Karrer KM, Peiffer SL, DiTomas ME (1993) Two distinct gene subfamilies within the family of cysteine protease genes. Proceedings of the National Academy of Sciences of the United States of America 90(7):3063-7
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