Identifiers and Description

Gene Model Identifier

TTHERM_00402050

Standard Name

COI12 (COnjugation-Induced 12)

Aliases

PreTt12749 | 43.m00267 | 3713.m00093

Description

anaphase-promoting complex cyclosome subunit 3; A Hsp90 co-chaperone required for the loading of scnRNAs to Twi1p

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

• ( PF07337 ) DC-EC Repeat
• ( PF00515 ) tetratricopeptide repeat
• ( PF07719 ) tetratricopeptide repeat
• ( PF08180 ) B melanoma antigen family
• ( PF02071 ) Aromatic-di-Alanine (AdAR) repeat

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.2%

TetraMine Data

TTHERM_00402050

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_13976.1	1.999872053281847e-142	hypothetical protein (544 aa)

General Information

Paragraph No	Gene Name	Paragraph Text
103	COI12	The Tetrahymena Hsp90 co-chaperone Coi12p promotes scnRNA loading into the Argonaute protein Twi1p in both ATP-dependent and ATP-independent manners.
105	GIW1, COI12, TWI1	ATP-dependent and ATP-independent activities of Coi12p facilitate scnRNA loading by counteracting the Twi1p-binding protein Giw1p, which is important for sorting scnRNAs to Twi1p (Woehrer et al. 2015).

Associated Literature

1. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
2. Ref:26688337: Kataoka K, Mochizuki K (2015) Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination. Developmental cell 35(6):775-88
3. Ref:25588944: Woehrer SL, Aronica L, Suhren JH, Busch CJ, Noto T, Mochizuki K (2015) A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms. The EMBO journal 34(4):559-77

Sequences

>TTHERM_00402050(coding)
ATGTATTACTTGCCTAAAAGATACTTTTCCAAATAAATCAAATTTCAAATCAAACTCAAA
TTTGGTAGGAAATTAAATTTTCAAGCTAAATAAACTGCCCTCCCCAATAACTTTAATATT
TACATGTAAATCTATTTGATTGATTTTTAATTATTTCGTTCAATATATATAGCTAGCTAG
CTAGATAGATTGCTTTAAAGTAATTCAATCAATTTCAACAAAAAGATAATTACTTTAATT
AGCAAGGCTATTTTAAATATTAAAACAAACACTATTATATTAAAAATTAAATAAATTATA
ATTAAGCATTATTTCATTAAAAAATAAATAAAAGACATTAAAATGGATTCATCTAATGAA
AACACAAGGAGCTCTTCTTGTAATGAGTAGGAAACTAATTCAGAACTTTAGTAAAGTGTT
AATGAGAGTTGTACCAGCCTAGAAGAGTAGTAATTCAGCACTCCTTTTTTATCCAGCCAA
GAAACTGTATAGGTAGACTAAGCATTCTAAGAATCCACTAAATAGAATGAAGGAACTTCT
TCTTTCTTAAACGAGACCTAATGCTATCAAGATTGTAAGCCCAATTTAATTTCTAAAGGA
GAGTCAAATCAACAGTAATACAAAGAGTAAACTCAAGAAATTAGTGAGATTTTAGAAAAC
CCTTTTGCTTAAAATACACCAACTGATTAATGGAGCATTGTAGAAGAAAAAGATGGTAAT
AAACAAGCAGATAGTTTTGATATTTTATAAGACAAATTGATTAAAGAGGAAACATATAAC
ATCCCTAGTTGGAATTATGAGCCTTAAGATTTTAAAGCAGATATTGACTTTGAAAAATAT
GACAAAGAAAATCTAGATCACCTAAAGAAAGTCTACAAGCCTCCATAAATTGAAGACGTC
GTCGAAAAAGTAGAATAAGATGAAAATGGGCTTAAAATCTAAACTTTATCTTAATTCAAT
ATACCTAAATGCAATATACTGAAGCTTGATATGAGTTTTCTCTAAACTTGGCAATCAAAA
GAAATAGTACCATGCTTTACTATGTTTTCAGAACCAAAAACTAACAACGGGGAAAGTCTA
AATTGTGATGAAGAAGAAACTAATCCTTAGATTGATAAGAAATGGTGCAGAGATGTTTAA
GTTGTCTTTGAAGAAAGAGATTAGAATGGTGAGCTTATTGATAAATATAGAGTTGTCAAA
GAAAAAAGAACAGTTAAATTATAAGGAGATATATCAGCAAAGTGGTATATTTGTCTAGAG
CAGATGAAGCCAAATGAAACTAAGTGGTTTAAAATGGAATAAAAACTTCTTTGGCAGATT
GGACAAAAAAATTTAGCTTAATATGAGTGTATTTATCTAAAAATATAAATTGTTGGAGTA
AAGGCTCTTGCTCTTTCTGAAATGAAAGATAAACTTGAAAAAAATAACTTACAACCCTAA
CAGTAAAATATAATCTATGAGGATTATAGTTAAATAGCTCAGAAATCAGAAGAGTTTAAC
AATGAAGGAAAAATTCTATTCTCTATTATGAATTATGAAAAAGCTGCAAAATGGTTTAAC
AAAGCATTTAGTATTTGGAGAGGAATGAGCAAAAAGCTTAGAAAAACTTTAAATTCTGAA
TAGGAATAGAATTATATTGATAAGAGTAACTAGTTTGGTGCTAATTATGCATAATGCCTT
ATTAACCTCAAAAAATATTAAGAGTGTGTGGATTTTTACTAAAAAAACAAGCTTTCTTCT
AATAAGAACATTTATAAACTTGTAAAAAGTTTATATGAATTACATAACTATGAAAAAGCT
ATTTAAGTAATAGATAAACAGTTTCCTTAAAATGATGAAAACTCAGTTGATAAATTTATG
AACGGTAATCAAGAAAGTTTACTTCCAGCTGAATTCTAAAAGCTTAAAAGTCAGTGTTAA
GTAAAGCTAAAAATGTTAGAAAATCAATCAATAAATACATTTTATAGAAAATTGTTTTAA
TGA

>TTHERM_00402050(protein)
MYYLPKRYFSKQIKFQIKLKFGRKLNFQAKQTALPNNFNIYMQIYLIDFQLFRSIYIASQ
LDRLLQSNSINFNKKIITLISKAILNIKTNTIILKIKQIIIKHYFIKKQIKDIKMDSSNE
NTRSSSCNEQETNSELQQSVNESCTSLEEQQFSTPFLSSQETVQVDQAFQESTKQNEGTS
SFLNETQCYQDCKPNLISKGESNQQQYKEQTQEISEILENPFAQNTPTDQWSIVEEKDGN
KQADSFDILQDKLIKEETYNIPSWNYEPQDFKADIDFEKYDKENLDHLKKVYKPPQIEDV
VEKVEQDENGLKIQTLSQFNIPKCNILKLDMSFLQTWQSKEIVPCFTMFSEPKTNNGESL
NCDEEETNPQIDKKWCRDVQVVFEERDQNGELIDKYRVVKEKRTVKLQGDISAKWYICLE
QMKPNETKWFKMEQKLLWQIGQKNLAQYECIYLKIQIVGVKALALSEMKDKLEKNNLQPQ
QQNIIYEDYSQIAQKSEEFNNEGKILFSIMNYEKAAKWFNKAFSIWRGMSKKLRKTLNSE
QEQNYIDKSNQFGANYAQCLINLKKYQECVDFYQKNKLSSNKNIYKLVKSLYELHNYEKA
IQVIDKQFPQNDENSVDKFMNGNQESLLPAEFQKLKSQCQVKLKMLENQSINTFYRKLFQ

>TTHERM_00402050(gene)
ATGTATTACTTGCCTAAAAGATACTTTTCCAAATAAATCAAATTTCAAATCAAACTCAAA
TTTGGTAGGAAATTAAATTTTCAAGCTAAATAAACTGCCCTCCCCAATAACTTTAATATT
TACATGTAAATCTATTTGATTGATTTTTAATTATTTCGTTCAATATATATAGCTAGCTAG
CTAGATAGATTGCTTTAAAGTAATTCAATCAATTTCAACAAAAAGATAATTACTTTAATT
AGCAAGGCTATTTTAAATATTAAAACAAACACTATTATATTAAAAATTAAATAAATTATA
ATTAAGCATTATTTCATTAAAAAATAAATAAAAGACATTAAAATGGATTCATCTAATGAA
AACACAAGGAGCTCTTCTTGTAATGAGTAGGAAACTAATTCAGAACTTTAGTAAAGTGTT
AATGAGAGTTGTACCAGCCTAGAAGAGTAGTAATTCAGCACTCCTTTTTTATCCAGCCAA
GAAACTGTATAGGTAGACTAAGCATTCTAAGAATCCACTAAATAGAATGAAGGAACTTCT
TCTTTCTTAAACGAGACCTAATGCTATCAAGATTGTAAGCCCAATTTAATTTCTAAAGGA
GAGTCAAATCAACAGTAATACAAAGAGTAAACTCAAGAAATTAGTGAGATTTTAGAAAAC
CCTTTTGCTTAAAATACACCAACTGATTAATGGAGCATTGTAGAAGAAAAAGATGGTAAT
AAACAAGCAGATAGTTTTGATATTTTATAAGACAAATTGATTAAAGAGGAAACATATAAC
ATCCCTAGTTGGAATTATGAGCCTTAAGATTTTAAAGCAGATATTGACTTTGAAAAATAT
GACAAAGAAAATCTAGATCACCTAAAGAAAGTCTACAAGCCTCCATAAATTGAAGACGTC
GTCGAAAAAGTAGAATAAGATGAAAATGGGCTTAAAATCTAAACTTTATCTTAATTCAAT
ATACCTAAATGCAATATACTGAAGCTTGATATGAGTTTTCTCTAAACTTGGCAATCAAAA
GAAATAGTACCATGCTTTACTATGTTTTCAGAACCAAGTAAGATTCATTCCAGTTTTAAA
CTACTTCTACTTACTTACTTACTTATTATTATTTTTAATTAATTAGATTTAGTTTTAATT
TTGTAATTTTTGAATTTTCTGAATCTTTTTTTTTCAGTTTTTAAAATTTAAAATGAATAA
ATTTAATTAATTTACAATAAATTAAAAAATTTTTAAACATTTTATTTGAAAATTGGCTAA
AATTTGTTATATAGAAATAAAAAATATTCCAAAATTGTTTATTTCATGAAATATTTTATT
TAAAAATTAATCTAGAAACTAACAACGGGGAAAGTCTAAATTGTGATGAAGAAGAAACTA
ATCCTTAGATTGATAAGAAATGGTGCAGAGATGTTTAAGTTGTCTTTGAAGAAAGAGATT
AGAATGGTGAGCTTATTGATAAATATAGAGTTGTCAAAGAAAAAAGAACAGTTAAATTAT
AAGGAGATATATCAGCAAAGTGGTATATTTGTCTAGAGCAGATGAAGCCAAATGAAACTA
AGTGGTTTAAAATGGAATAAAAACTTCTTTGGCAGATTGGACAAAAAAATTTAGCTTAAT
ATGAGTGTATTTATCTAAAAATATAAATTGTTGGAGTAAAGGCTCTTGCTCTTTCTGAAA
TGAAAGATAAACTTGAAAAAAATAACTTACAACCCTAACAGTAAAATATAATCTATGAGG
ATTATAGTTAAATAGCTCAGAAATCAGAAGAGTTTAACAATGAAGGAAAAATTCTATTCT
CTATTATGAATTATGAAAAAGCTGCAAAATGGTTTAACAAAGCATTTAGTATTTGGAGAG
GAATGAGCAAAAAGCTTAGAAAAACTTTAAATTCTGAATAGGAATAGAATTATATTGATA
AGAGTAACTAGTTTGGTGCTAATTATGCATAATGCCTTATTAACCTCAAAAAATATTAAG
AGTGTGTGGATTTTTACTAAAAAAACAAGCTTTCTTCTAATAAGAACATTTATAAACTTG
TAAAAAGTTTATATGAATTACATAACTATGAAAAAGCTATTTAAGTAATAGATAAACAGT
TTCCTTAAAATGATGAAAACTCAGTTGATAAATTTATGAACGGTAATCAAGAAAGTTTAC
TTCCAGCTGAATTCTAAAAGCTTAAAAGTCAGTGTTAAGTAAAGCTAAAAATGTTAGAAA
ATCAATCAATAAATACATTTTATAGAAAATTGTTTTAATGA