Identifiers and Description

Gene Model Identifier

TTHERM_00384910

Standard Name

CEN1 (CENtrin )

Aliases

PreTt24783 | 41.m00190 | centrin

Description

Centrin; EF Hand Ca2+ binding protein; alters MT sliding velocity to regulate ciliary beat form; localizes to basal bodies, oral apparatus cortical fibers, ciliary rootlets, the apical filament ring, and axonemal 14S inner arm dynein

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• microtubule basal body (IDA) | GO:0005932 | Ref:17785518
• oral apparatus (IDA) | GO:0031912 | Ref:12529441
• axoneme (IDA) | GO:0005930 | Ref:12529441

Molecular Function

• calcium ion binding (ISS) | GO:0005509 | Ref:12529441

Domains

• ( PF00036 ) EF hand
• ( PF07813 ) LTXXQ motif
• ( TIGR01784 ) conserved hypothetical protein

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.4%

TetraMine Data

TTHERM_00384910

WebApollo

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Tetrahymena Stock Center

No Data fetched for StockID

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Tetrahymena borealis	EI9_04411.1	9.004285235721242e-114	EF hand family protein (168 aa )
Oxytricha	Contig13931.0.g32	9.999030951775615e-88	EF hand
SGD	YOR257W	6.997794455893953e-54	CDC31 Calcium-binding componen t of the spindle pole body (SP B) half-bridge, required for S PB duplication in mitosis and meiosis II; homolog of mammali an centrin; binds multiubiquit inated proteins and is involve d in proteasomal protein degra dation
WormBase	WBGene00000552	2.0008536325002377e-42	locus:cmd-1 Calmodulin sta tus:Confirmed UniProt:O16305 protein_id:CCD69969.1
DictyBase	DDB_G0279407	4.001118334825066e-42	calA on chromosome: 3 position 2244074 to 2245039
Stentor Coeruleus	SteCoe_19772	2.2273635617957438e-39

General Information

No Data fetched for General Information

Associated Literature

1. Ref:17785518: Kilburn CL, Pearson CG, Romijn EP, Meehl JB, Giddings TH, Culver BP, Yates JR, Winey M (2007) New Tetrahymena basal body protein components identify basal body domain structure. The Journal of cell biology 178(6):905-12
2. Ref:16228897: Libusová L, Sulimenko T, Sulimenko V, Janisch R, Hozák P, Dráber P (2005) Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex. Protoplasma 225(3-4):157-67
3. Ref:15944224: Stemm-Wolf AJ, Morgan G, Giddings TH, White EA, Marchione R, McDonald HB, Winey M (2005) Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family. Molecular biology of the cell 16(8):3606-19
4. Ref:15134263: McLaughlin NB, Buhse HE (2004) Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of Tetrahymena vorax. The Journal of eukaryotic microbiology 51(2):253-7
5. Ref:12529441: Guerra C, Wada Y, Leick V, Bell A, Satir P (2003) Cloning, localization, and axonemal function of Tetrahymena centrin. Molecular biology of the cell 14(1):251-61
6. Ref:12356864: Shang Y, Li B, Gorovsky MA (2002) Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. The Journal of cell biology 158(7):1195-206

Sequences

>TTHERM_00384910(coding)
ATGTCCCTCAAGAACACTAGACCCCCTGTTAAGTCCACCAGAGTCTCTCGTCCTCAATTG
ACTGAAGAATAAAAATAAGAAATTAAGGAAGCCTTCGATCTTTTCGATACCGATGGCACT
GGATATATTGATGCCAAGGAACTTAAGGTAGCAATGAGAGCTTTAGGATTTGAACCTAAA
AAGGAAGAAATCAAGAAAATGATTGCTGAAATCGACAGAGAAGGTCGTGGAGTTATTGAA
TTCTAAGACTTCCTTGATCTCATGACCGTTAAAATGGCTGAAAGAGATCCCAGAGATGAA
ATTTTAAAAGCTTTTAGATTATTTGATGATGATAATACTGGTAAGATCTCCTTAAAGAAT
TTAAAAAGAGTTGCCAGAGAATTGGGTGAAGCTATGACTGAAGAAGAACTCTAAGAAATG
ATTGATGAAGCTGATAGAGATGGTGATGGTGAAATTAGTGAGGAAGAATTCGTTAGAATT
ATGAAAAAGACCAACCTCTTCTGA

>TTHERM_00384910(protein)
MSLKNTRPPVKSTRVSRPQLTEEQKQEIKEAFDLFDTDGTGYIDAKELKVAMRALGFEPK
KEEIKKMIAEIDREGRGVIEFQDFLDLMTVKMAERDPRDEILKAFRLFDDDNTGKISLKN
LKRVARELGEAMTEEELQEMIDEADRDGDGEISEEEFVRIMKKTNLF

>TTHERM_00384910(gene)
ATGTCCCTCAAGAACACTAGACCCCCTGTTAAGTCCACCAGAGTCTCTCGTCCTCAATTG
ACTGAAGAATAAAAATAAGAAATTAAGGAAGCCTTCGATCTTTTCGATACCGATGGCACT
GGATATATTGATGCCAAGGAACTTAAGGTAGCAATGAGAGCTTTAGGATTTGAACCTAAA
AAGGAAGAAATCAAGAAAATGATTGCTGAAATCGACAGAGAAGGTCGTGGAGTTATTGAA
TTCTAAGACTTCCTTGATCTCATGACCGTTAAAATGGTAACTAAAATTTCTTTATTTTAA
TAAATAAGCAGACAGACATACTCATGAACTAGCATTAATAATTTGAATTTAAATAAAATA
GGCTGAAAGAGATCCCAGAGATGAAATTTTAAAAGCTTTTAGATTATTTGATGATGATAA
TACTGGTAAGATCTCCTTAAAGAATTTAAAAAGAGTTGCCAGAGAATTGGGTGAAGCTAT
GACTGAAGAAGAACTCTAAGAAATGATTGATGAAGCTGATAGAGATGGTGATGGTGAAAT
TAGTGAGGAAGAATTCGTTAGAATTATGAAAAAGACCAACCTCTTCTGA