Identifiers and Description

Gene Model Identifier

TTHERM_01104960

Standard Name

BLT1 (Beta-Like Tubulin 1)

Aliases

PreTt01888 | 235.m00039

Description

Highly divergent beta-like tubulin; present in very low abundance; localizes to small subset of microtubules; not incorporated into growing ciliary axonemes

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• protein complex (IEA) | GO:0043234

Molecular Function

• GTP binding (IEA) | GO:0005525
• GTPase activity (IEA) | GO:0003924

Biological Process

• protein polymerization (IEA) | GO:0051258

Domains

• ( PF00091 ) Tubulin/FtsZ family, GTPase domain
• ( PF03953 ) tubulin/FtsZ family, C-terminal domain

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.1%

TetraMine Data

TTHERM_01104960

WebApollo

Fullscreen View

Tetrahymena Stock Center

• ( SD02243 ) Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02244 ) Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02245 ) Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02246 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02247 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02248 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02249 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02250 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02251 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02252 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02253 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02254 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02255 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02256 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02257 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02258 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02259 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02260 ) Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02261 ) Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02262 ) Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02562 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02563 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02564 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02565 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02566 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02567 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Stentor Coeruleus	SteCoe_5719	0
WormBase	WBGene00006538	9.997093137033517e-262	locus:tbb-4 Tubulin beta-4 c hain status:Partially_confir med UniProt:P41937 protein _id:CAA86310.1
Tetrahymena borealis	EI9_16521.1	9.997093137033517e-262	tubulin/FtsZ family, GTPase do main-containing protein (472 a a)
DictyBase	DDB_G0269196	9.997093137033517e-262	tubB on chromosome: 1 position 3261649 to 3263130
Oxytricha	Contig8792.0.g74	9.997093137033517e-262	Tubulin/FtsZ family, GTPase do main
SGD	YFL037W	9.997093137033517e-262	TUB2 Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form micr otubules

General Information

No Data fetched for General Information

Associated Literature

1. Ref:16933976: Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, Collins K, Stewart BA, Lee SR, Wilamowska K, Weinberg Z, Ruzzo WL, Wloga D, Gaertig J, Frankel J, Tsao CC, Gorovsky MA, Keeling PJ, Waller RF, Patron NJ, Cherry JM, Stover NA,
2. Ref:11891286: Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 99(6):3734-9

Sequences

>TTHERM_01104960(coding)
ATGAGAGAAATTGTTTGCATTTCAGCAGGTCAATGCGGTAACTAAATAGGATATAAGTTC
TGGGAAAGAGTTTCTCAAGAGCATGGAGTCGAAGAGACTGGTCTGTATAACGGAAATAAT
GACTTATAGCTCGAGAGAATTGATGTTTACTATAATGAAGGCTCAAGTGGACGTTATGTA
CCTAGATCAGTAATGGTTGATTTGGAACCTGGTGTATTGGATTCAATTCGTGCATCAAAA
TACAGATAACTTTTCCATCCTGATAATTTCATTCACGCTCAAAGTAGCGCTTCTAATAAT
TGGGCTAAGGGACACTATTCTGAAGGTGCTGAACTTTAATAGGAAGTTTTAGATATTGTC
CGTAGAGAAGTTGAAGGTTGTGACAGCCTATAAGGTTTCCAATTCACTCATTCTATTGGT
GGAGGCACTGGAAGTGGTATGGGCACTTTGCTTATTTAAAAGCTTAGAGAAGAATATCCA
GACAGAATTTTTTCAAGTTTTTCTGTTTTCCCATCCGTAAAAGTAAGCGATGTTGTAATT
GAACCTTATAATGCTACATTGAGCATTCATTAGCTCATAGAAAATGTTGACTAATGCATG
GTTATAGATAATGAAGCTTTATACAATATTTGCATGAAAAATTTAAAGATTGAACACCCT
ACTTATGGTGACCTCAATCATATTGTCTCTGTAGCTATGTCTGGTGTTACTTGCAGTATG
AGATTCCCTGGATAATTAAATGCAGATCTCAGAAAATTGTCAGTTAATTTGACTCCCTTC
CCTCGTTTACACTTCTTTACAGTTGGAATAGCTCCTTTATATGGAAGAGGATAGGCTTAG
TTTGCCAGTAAATATGATGTTCCTGAAATTCTTTAGCAAGCTTTTAATCCTCACAATATG
ATGTGTGCTGCCGATGTTTCTCATGGAAAATATTTAACAGCTGCTCTTGCTTTTAGAGGA
AAAGTATCATCATATGAAATCGAAAAGCAACTACTAAACTTCTAACAAAAAAATAGCAAC
CGTTTTACAGAATGGATTCCAAACAGTATGAAGCTAAGTATGTGCGATATATCCTTGGAA
TAAGTACCAAGAAGCGCTGTTTTAATAAGTAATAACACATCGATATAAGAAGTATTCAAA
AGAATAAATGCTTAATATACAAGCATGTTTAGAAGAAAGGCTTTCGTTCACTGGTATTTA
GATGAAGGTATGGACGAAATGGAGTTTAATGAAGCAGAAGCTAATATGAATGACTTGGTG
AGTGAATATCAATAATACTAAGATTCAACCGTAGACAGTGAAGAAGAAATTGACTTAGAT
TAGACATAGGCAACATTTGGAGAAAGATAAGAAAAACAAAATAAAAAATGTAACTCAAAA
TAACAATCTAATATTGCTTCATCAAATGAAATTTGA

>TTHERM_01104960(protein)
MREIVCISAGQCGNQIGYKFWERVSQEHGVEETGLYNGNNDLQLERIDVYYNEGSSGRYV
PRSVMVDLEPGVLDSIRASKYRQLFHPDNFIHAQSSASNNWAKGHYSEGAELQQEVLDIV
RREVEGCDSLQGFQFTHSIGGGTGSGMGTLLIQKLREEYPDRIFSSFSVFPSVKVSDVVI
EPYNATLSIHQLIENVDQCMVIDNEALYNICMKNLKIEHPTYGDLNHIVSVAMSGVTCSM
RFPGQLNADLRKLSVNLTPFPRLHFFTVGIAPLYGRGQAQFASKYDVPEILQQAFNPHNM
MCAADVSHGKYLTAALAFRGKVSSYEIEKQLLNFQQKNSNRFTEWIPNSMKLSMCDISLE
QVPRSAVLISNNTSIQEVFKRINAQYTSMFRRKAFVHWYLDEGMDEMEFNEAEANMNDLV
SEYQQYQDSTVDSEEEIDLDQTQATFGERQEKQNKKCNSKQQSNIASSNEI

>TTHERM_01104960(gene)
ATGAGAGAAATTGTTTGCATTTCAGCAGGTCAATGCGGTAACTAAATAGGATATAAGTTC
TGGGAAAGAGTTTCTCAAGAGCATGGAGTCGAAGAGACTGGTCTGTATAACGGAAATAAT
GACTTATAGCTCGAGAGAATTGATGTTTACTATAATGAAGGCTCAAGTGGACGTTATGTA
CCTAGATCAGTAATGGTTGATTTGGAACCTGGTGTATTGGATTCAATTCGTGCATCAAAA
TACAGATAACTTTTCCATCCTGATAATTTCATTCACGCTCAAAGTAGCGCTTCTAATAAT
TGGGCTAAGGGACACTATTCTGAAGGTGCTGAACTTTAATAGGAAGTTTTAGATATTGTC
CGTAGAGAAGTTGAAGGTTGTGACAGCCTATAAGGTTTCCAATTCACTCATTCTATTGGT
GGAGGCACTGGAAGTGGTATGGGCACTTTGCTTATTTAAAAGCTTAGAGAAGAATATCCA
GACAGAATTTTTTCAAGTTTTTCTGTTTTCCCATCCGTAAAAGTAAGCGATGTTGTAATT
GAACCTTATAATGCTACATTGAGCATTCATTAGCTCATAGAAAATGTTGACTAATGCATG
GTTATAGATAATGAAGCTTTATACAATATTTGCATGAAAAATTTAAAGATTGAACACCCT
ACTTATGGTGACCTCAATCATATTGTCTCTGTAGCTATGTCTGGTGTTACTTGCAGTATG
AGATTCCCTGGATAATTAAATGCAGATCTCAGAAAATTGTCAGTTAATTTGACTCCCTTC
CCTCGTTTACACTTCTTTACAGTTGGAATAGCTCCTTTATATGGAAGAGGATAGGCTTAG
TTTGCCAGTAAATATGATGTTCCTGAAATTCTTTAGCAAGCTTTTAATCCTCACAATATG
ATGTGTGCTGCCGATGTTTCTCATGGAAAATATTTAACAGCTGCTCTTGCTTTTAGAGGA
AAAGTATCATCATATGAAATCGAAAAGCAACTACTAAACTTCTAACAAAAAAATAGCAAC
CGTTTTACAGAATGGATTCCAAACAGTATGAAGCTAAGTATGTGCGATATATCCTTGGAA
TAAGTACCAAGAAGCGCTGTTTTAATAAGTAATAACACATCGATATAAGAAGTATTCAAA
AGAATAAATGCTTAATATACAAGCATGTTTAGAAGAAAGGCTTTCGTTCACTGGTATTTA
GATGAAGGTATGGACGAAATGGAGTTTAATGAAGCAGAAGCTAATATGAATGACTTGGTG
AGTGAATATCAATAATACTAAGATTCAACCGTAGACAGTGAAGAAGAAATTGACTTAGAT
TAGACATAGGCAACATTTGGAGAAAGATAAGAAAAACAAAATAAAAAATGTAACTCAAAA
TAACAATCTAATATTGCTTCATCAAATGAAATTTGA