Gene Model Identifier
TTHERM_00191480
Standard Name
ASI2
Aliases
PreTt08799 | 15.m00510 | 3697.m00221
Description
Similar to anlagen stage induced protein 1 (ASI1); protein of unknown function; gene is located adjacent to the ASI1 gene
Genome Browser (Macronucleus)
Genome Browser (Micronucleus)
Cellular Component Molecular Function Biological Process
( PF00672 ) HAMP domain
( PF02743 ) Cache domain
( PF02326 ) YMF19 hypothetical plant mitochondrial protein
Tetrahymena Functional Genomics Database:
Signal Peptide Present?: No
TTHERM_00191480
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( SD01594 ) Macronucleus: ASI2 replaced with neo2 cassette ( SD01595 ) Macronucleus: ASI2 replaced with neo2 cassette ( SD01596 ) ( SD01597 ) ( SD03069 ) Micronucleus: heterozygous for ASI2 knockout ( SD03070 ) Micronucleus: ASI2 ( SD03071 ) Macronucleus: ASI2 somatic knockout ( SD03072 ) Macronucleus: ASI2 knockout ( SD03073 ) Macronucleus: ASI2 replaced with ASI2Y607F
(v.2006 protein sequences)
Source Identifier Score Description
Tetrahymena borealis EI9_06168.1 9.997093137033517e-262 ASI2 protein (724 aa)
Paragraph No Gene Name Paragraph Text 85 ASI2 Gene Information:
ASI2 is a gene regulating endocycling in Tetrahymena thermophila. Though nonessential for vegative growth, it is upregulated after meiosis and is involved in the creation of a new MAC. Introduced via transduction with ASI2-GFP plasmid to establish parental cells that showed transcription of ASI2 occurs both in MAC and parental cells at conjugation.
86 ASI2 Nucleic Acid Interactions:
ASI2p independent of ssRNA synthesis/accumulation
87 ASI2 Regulation & Expression:
The Tetrahymena gene ASI1 (anlagen stage induced 1) was isolated from a cDNA library of genes that are up-regulated during development of the macronuclear anlagen. As its name implies, the abundance of ASI2 mRNA peaks at 9 h of mating, early in macronuclear anlagen development.
Ref:20656911 : Yin L, Gater ST, Karrer KM (2010) A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena. Eukaryotic cell 9(9):1343-53Ref:16712831 : Li S, Yin L, Cole ES, Udani RA, Karrer KM (2006) Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth. Developmental biology 295(2):633-46
>TTHERM_00191480(coding)ATG ACA CAA ACG GAA CTC GTT CCT GAA GAT TAT GAC CAG GAT AAT TAA GAT GCT TCT CAA AGC GTA ATC TCT TCA GGA AGC GTT GAA GAT GGA AAA GAA AAA TTT AAA GCA ATG ATA CAT AAA ATT TTA GGA GTA AAC GAT AAC AAA TAA ATA GAT AAA ATA TGC TGT TTC TCC ATT TTT AAT AAG CAG TGG TCT CGT ACA AAG CAA CTG TTT TGT GTT TAC TCA GTC TTT TTG ACA TTA TTT ATG ACT ATA ATA TTA GGG TGT TTT GTG TTC TAC TTA AAA TTT TGT CCC AAT TTC GTA GGA GAT ACA ATC TTA GAG TAG ATT GAT AAC TCA ATT TCT GCA AGC TTT TAA AAT TCC TTA CTT GAA TAC AGT AAA TAA GTA CAT TAA TAT TTC AAA GCA TAT AGT TCT TGG ATG ATT ATG TTC TCC CAA ATA GTA GCT AAC AAT GTT GGT TGG AAT AAT TCA CTA TAT TAA TCC TCA AAC GAA TTA AAT TTA TAA CCA CAA CAA AAA TAT TTT CCT ATT TAT TAG CCT ATC CCA TAA AAT ATA GTA AAT AAG TAA TAT TCT TAG AAA AAG CCC TAT TAT GAT AAA GAT ATA TCA TTT TAA ACT TTT TCT TAT TTT CAC AAC CAT CCT GAC GAT GCA ACT ACT ATT TCA CCA GCT ACT TAA ACA TAT TTG GAC TCT GTA GAC TTG ATT TCT TAT TTT TTT TCT AAT TTT ATA TAA GAT CCT AAT TTA ACT TAT GTC TAG TCT TTA TAT ATT TAC CTT TAA GAT CGA GAT GTC ATT TGT TTT TTT CCA GCA GGC CTC ATT AAA GTC CCA AAA GAT TTT AAG ATA GAT ATG AAA TAA AGG CCT TGG TAT TTA AAT ACT GCT TAA ACA TTT TAA GCC AAT GGA AAT AAA CCT GTA TTT ACA ATT ACC AGT CCC TAT CCT GAT TTA ACT ACT AAA TAA ATA ACT ATA ACA TAC TCC CTT GCA ATC GTC AAA CAG TCA GAT CCT ACT TAA ATT GCT GGA GTT ATT AAT GTA GAT GTT ATT GTT GGT AAT TTT AGA TCT GTT TAT GAC TTG CTG CAG GAA AAA GAT GGT GAA TTT TAA ATT GCC TTA GTA GAT TAT AAT ACA GAA TAC ATC ATT AAT GAT TGC TTT AAT TAA AGT GGA ATT TCA CAT TTT TAT GAT ACG AAT AAT ACA AAC TTT ACA CTT GAA AAT TGG AAT GAA ATC AAA GAT TTT AAT TAA CAC CAA TAA AGT TAA ACA TTC ATC ATT ACT AAT AGT TAG AAT GTA AGA TAT TAT TTT GCT CGT ACT TTA ATA AAA GAT ACA GTA AAT CAA ACA TTA AAT AGA TTT TAT CTT ATC GTC TTT GGT AAA GAA AGC AAT TAT CTC TCC TAT AGA AAC AAT ATT TAA GAT AGA ATA TAT AAT GAG CTC TAT AAC TTT TGC ATA TCA TTA ATA ATT GCT TTT TCT ATT TTG TGG TTC TTT TCA TAT TTC TAT ATC TAT GCT CTA TCA CGC AAT ATT GTT CTA CCG CTT TTT AAA TTG AGT TAA TTA GCT AAA AGT ATA AAC AAG CAC GAT GAG AGA AAA AGC TCT GCT GAA GAC TTG AAA GAA TAG TTA GGT AAG ATT TAA GGT GCA GAT ACT GAT ACA AAA AAG CTT ATC GAT TCT TTC ATT AAC CTT GTA GAT TAG CAC TCT GGC ATG AGA AAA CTA GCA AAT GGT AAT GGT TAC TAA GAC AAT ATG ATC CCA GTC TAC AAT AGA ATG AGC AAC ATT TAT GAA AAC TAT TCT AGG AAT GAC TAC AGA GAA GAA TTG GAT GAT ATA TAA GAA TAA ATT AAA TAA TTA AAG AAT TTA GAT TAG GAA GAT GAA GAT GAT TAT GAT GAA AAT GGA TCT AAT TAA GAT ATT GAT GAA GTC TAT GCT GGA AAT CCT AAA AAT AAA TCT TAT TCA TCA ATT TAA TCT AAT AAA TAT TAA AAC GAA GAT GAT TAA ACA GAA GCT GAT CTT AGA AAT CAT AAA AAA AAG TTA GAG TTA GAA GCG AAA GGA GGC AAC CGT ACT TCA ACA AAT TCT TTA GCT AGT AAT TTA GAA AAC TAT AAT AAA AGA AAA TAT CTA AAT TGG ATT TAA ACT TAA CTC TAA GTA ATA CAA GAA AAC GAA TAA GCT AAA ATA AAG TTG TCT CAA TAA ATT TTA TAG TAA TTT TTA CCT ACA AAT ACT TAA TCT ATT TGA >TTHERM_00191480(protein)M T Q T E L V P E D Y D Q D N Q D A S Q S V I S S G S V E D G K E K F K A M I H K I L G V N D N K Q I D K I C C F S I F N K Q W S R T K Q L F C V Y S V F L T L F M T I I L G C F V F Y L K F C P N F V G D T I L E Q I D N S I S A S F Q N S L L E Y S K Q V H Q Y F K A Y S S W M I M F S Q I V A N N V G W N N S L Y Q S S N E L N L Q P Q Q K Y F P I Y Q P I P Q N I V N K Q Y S Q K K P Y Y D K D I S F Q T F S Y F H N H P D D A T T I S P A T Q T Y L D S V D L I S Y F F S N F I Q D P N L T Y V Q S L Y I Y L Q D R D V I C F F P A G L I K V P K D F K I D M K Q R P W Y L N T A Q T F Q A N G N K P V F T I T S P Y P D L T T K Q I T I T Y S L A I V K Q S D P T Q I A G V I N V D V I V G N F R S V Y D L L Q E K D G E F Q I A L V D Y N T E Y I I N D C F N Q S G I S H F Y D T N N T N F T L E N W N E I K D F N Q H Q Q S Q T F I I T N S Q N V R Y Y F A R T L I K D T V N Q T L N R F Y L I V F G K E S N Y L S Y R N N I Q D R I Y N E L Y N F C I S L I I A F S I L W F F S Y F Y I Y A L S R N I V L P L F K L S Q L A K S I N K H D E R K S S A E D L K E Q L G K I Q G A D T D T K K L I D S F I N L V D Q H S G M R K L A N G N G Y Q D N M I P V Y N R M S N I Y E N Y S R N D Y R E E L D D I Q E Q I K Q L K N L D Q E D E D D Y D E N G S N Q D I D E V Y A G N P K N K S Y S S I Q S N K Y Q N E D D Q T E A D L R N H K K K L E L E A K G G N R T S T N S L A S N L E N Y N K R K Y L N W I Q T Q L Q V I Q E N E Q A K I K L S Q Q I L Q Q F L P T N T Q S I >TTHERM_00191480(gene)ATG ACA CAA ACG GAA CTC GTT CCT GAA GAT TAT GAC CAG GAT AAT TAA GAT GCT TCT CAA AGC GTA ATC TCT TCA GGA AGC GTT GAA GAT GGA AAA GAA AAA TTT AAA GCA ATG ATA CAT AAA ATT TTA GGA GTA AAC GAT AAC AAA TAA ATA GAT AAA ATA TGC TGT TTC TCC ATT TTT AAT AAG CAG TGG TCT CGT ACA AAG CAA CTG TTT TGT GTT TAC TCA GTC TTT TTG ACA TTA TTT ATG ACT ATA ATA TTA GGG TGT TTT GTG TTC TAC TTA AAA TTT TGT CCC AAT TTC GTA GGA GAT ACA ATC TTA GAG TAG ATT GAT AAC TCA ATT TCT GCA AGC TTT TAA AAT TCC TTA CTT GAA TAC AGT AAA TAA GTA CAT TAA TAT TTC AAA GCA TAT AGT TCT TGG ATG ATT ATG TTC TCC CAA ATA GTA GCT AAC AAT GTT GGT TGG AAT AAT TCA CTA TAT TAA TCC TCA AAC GAA TTA AAT TTA TAA CCA CAA CAA AAA TAT TTT CCT ATT TAT TAG CCT ATC CCA TAA AAT ATA GTA AAT AAG TAA TAT TCT TAG AAA AAG CCC TAT TAT GAT AAA GAT ATA TCA TTT TAA ACT TTT TCT TAT TTT CAC AAC CAT CCT GAC GAT GCA ACT ACT ATT TCA CCA GCT ACT TAA ACA TAT TTG GAC TCT GTA GAC TTG ATT TCT TAT TTT TTT TCT AAT TTT ATA TAA GAT CCT AAT TTA ACT TAT GTC TAG TCT TTA TAT ATT TAC CTT TAA GAT CGA GAT GTC ATT TGT TTT TTT CCA GCA GGC CTC ATT AAA GTC CCA AAA GAT TTT AAG ATA GAT ATG AAA TAA AGG CCT TGG TAT TTA AAT ACT GCT TAA ACA TTT TAA GCC AAT GGA AAT AAA CCT GTA TTT ACA ATT ACC AGT CCC TAT CCT GAT TTA ACT ACT AAA TAA ATA ACT ATA ACA TAC TCC CTT GCA ATC GTC AAA CAG GTA ATT CAT AAA TAA ATC AAT ATT TCT ATT ATT TTT AAT TTT GAC AAT TTA AAA TGC AAA TAA CTA AAT AAT AAT ATT TAT TTA TTT ATT TAT TTG CTT TTT GAT TTT TCA GTG GGT TAA TTA ATT TAA AAT TTT TAA TTA AAA TAG TCA GAT CCT ACT TAA ATT GCT GGA GTT ATT AAT GTA GAT GTT ATT GTT GGT AAT TTT AGA TCT GTT TAT GAC TTG CTG CAG GAA AAA GAT GGT GAA TTT TAA ATT GCC TTA GTA GAT TAT AAT ACA GAA TAC ATC ATT AAT GAT TGC TTT AAT TAA AGT GGA ATT TCA CAT TTT TAT GAT ACG AAT AAT ACA AAC TTT ACA CTT GAA AAT TGG AAT GAA ATC AAA GAT TTT AAT TAA CAC CAA TAA AGT TAA ACA TTC ATC ATT ACT AAT AGT TAG AAT GTA AGA TAT TAT TTT GCT CGT ACT TTA ATA AAA GAT ACA GTA AAT CAA ACA TTA AAT AGA TTT TAT CTT ATC GTC TTT GGT AAA GAA AGC AAT TAT CTC TCC TAT AGA AAC AAT ATT TAA GAT AGA ATA TAT AAT GAG CTC TAT AAC TTT TGC ATA TCA TTA ATA ATT GCT TTT TCT ATT TTG TGG TTC TTT TCA TAT TTC TAT ATC TAT GCT CTA TCA CGC AAT ATT GTT CTA CCG CTT TTT AAA TTG AGT TAA TTA GCT AAA AGT ATA AAC AAG CAC GAT GAG AGA AAA AGC TCT GCT GAA GAC TTG AAA GAA TAG TTA GGT AAG ATT TAA GGT GCA GAT ACT GAT ACA AAA AAG CTT ATC GAT TCT TTC ATT AAC CTT GTA GAT TAG CAC TCT GGC ATG AGA AAA CTA GCA AAT GGT AAT GGT TAC TAA GAC AAT ATG ATC CCA GTC TAC AAT AGA ATG AGC AAC ATT TAT GAA AAC TAT TCT AGG AAT GAC TAC AGA GAA GAA TTG GAT GAT ATA TAA GAA TAA ATT AAA TAA TTA AAG AAT TTA GAT TAG GAA GAT GAA GAT GAT TAT GAT GAA AAT GGA TCT AAT TAA GAT ATT GAT GAA GTC TAT GCT GGA AAT CCT AAA AAT AAA TCT TAT TCA TCA ATT TAA TCT AAT AAA TAT TAA AAC GAA GAT GAT TAA ACA GAA GCT GAT CTT AGA AAT CAT AAA AAA AAG TTA GAG TTA GAA GCG AAA GGA GGC AAC CGT ACT TCA ACA AAT TCT TTA GCT AGT AAT TTA GAA AAC TAT AAT AAA AGA AAA TAT CTA AAT TGG ATT TAA ACT TAA CTC TAA GTA ATA CAA GAA AAC GAA TAA GCT AAA ATA AAG TTG TCT CAA TAA ATT TTA TAG TAA TTT TTA CCT ACA AAT ACT TAA TCT ATT TGA
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