Identifiers and Description

Gene Model Identifier

TTHERM_00190950

Standard Name

ACT1 (ACTin 1)

Aliases

PreTt08746 | 15.m00457 | actin | 3697.m00168

Description

ACT1 macronuclear actin; Actin; structural protein; monomeric subunits of the actin protein (G-actin) polymerize to form structural filiaments (F-actin); localized to the cleavage forrow during cytokinesis

Genome Browser (Macronucleus)

Genome Browser (Micronucleus)

Gene Ontology Annotations

Cellular Component

• cleavage furrow (IDA) | GO:0032154 | Ref:3323192
• actin filament (TAS) | GO:0005884 | Ref:14733431
• cytoproct (IDA) | GO:0031911 | Ref:3323192
• actomyosin contractile ring (IDA) | GO:0005826 | Ref:14733431
• phagocytic vesicle (IDA) | GO:0045335 | Ref:3323192
• microtubule basal body (IDA) | GO:0005932 | Ref:14733431

Biological Process

• cytokinesis (IMP) | GO:0000910 | Ref:14733431
• exocytosis (IEP) | GO:0006887 | Ref:3323192
• phagocytosis (IMP) | GO:0006909 | Ref:16524910
• cilium or flagellum-dependent cell motility (IMP) | GO:0001539 | Ref:16524910

Domains

• ( PF00022 ) Actin

Gene Expression Profile

• Tetrahymena Functional Genomics Database:

Change in Gene Expression

TMHMM

TMHMM

Signal Peptide

Signal Peptide Present?: No
Presense Probability: 10.4%

TetraMine Data

TTHERM_00190950

WebApollo

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Tetrahymena Stock Center

• ( SD01796 ) ACT1 KO homozygous in mic, heterokaryon
• ( SD01797 ) ACT1 with neo3 inserted at Sfu1 site
• ( SD01798 ) ACT1 with neo3 inserted at Sfu1 site

Homologs (v.2006 protein sequences)

Source	Identifier	Score	Description
Stentor Coeruleus	SteCoe_32251	0
WormBase	WBGene00000066	9.997093137033517e-262	locus:act-4 Actin-4 status :Confirmed UniProt:P10986 protein_id:CCD83476.1
Tetrahymena borealis	EI9_06202.1	9.997093137033517e-262	actin, macronuclear (377 aa)
DictyBase	DDB_G0275023	9.997093137033517e-262	act22 on chromosome: 2 positio n 5343575 to 5344705
Oxytricha	Contig4562.0.g74	9.997093137033517e-262	Actin
SGD	YFL039C	9.997093137033517e-262	ACT1 Actin, structural protein involved in cell polarization , endocytosis, and other cytos keletal functions

General Information

No Data fetched for General Information

Associated Literature

1. Ref:16524910: Williams NE, Tsao CC, Bowen J, Hehman GL, Williams RJ, Frankel J (2006) The actin gene ACT1 is required for phagocytosis, motility, and cell separation of Tetrahymena thermophila. Eukaryotic cell 5(3):555-67
2. Ref:16593729: Cupples CG, Pearlman RE (1986) Isolation and characterization of the actin gene from Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 83(14):5160-4

Sequences

>TTHERM_00190950(coding)
ATGGCTGAAAGTGAATCCCCCGCTATTGTTATTGATAACGGTTCCGGTATGTGCAAGGCT
GGTATTGCTGGTGACGATGCTCCCAGAGCCGCCTTCCCCTCCATTGTCGGTAGACCCAAA
ATGCCTGGTATCATGGTCGGTATGGATCAAAAGGAATGCTACGTTGGTGAAGAAGCCCAA
GCCAAGAGAGGTGTTTTGAATTTGAAATATCCCATTGAACACGGTATTGTCACCGATTAC
GATGATATGGAAAAGATCTGGCATCACTGCTTCTACAATGAACTCAGAGTTACCCCTGAA
GAACACCCCTGCTTACTTACTGAAGCTCCCCAAAACCCCAAGTTGAACAGAGAAAAGATG
ACCAAGACCATGTTCGAAACTTTCAACGTTCCCTCCTTCTACGTTGCCATCCAAGCCGTT
CTTTCCCTCTACGCTTCCGGTAGAACCACTGGTATCGTCGTTGACTCTGGTGATGGTGTT
ACCCACACTGTTCCCATCTATGAAGGTTACGCTCTTCCTCACGCCATCTTGAGAATCGAT
TTGGCTGGTAGAGACTTAACTGAATACTGCATGAAGCTCCTCTACGAAATTGGTTTGAAC
TTCTCTTCCACTGCCGAAAGAGAAATCATCAGAGATATCAAGGAAAAGCTCTGCTACGTC
GCTATCGACTACGAAGCTGAATTAAAGGCTTACAAGGAATCCTCCTAAAACGATAAATCT
TACGAATTACCCGATGGTAACACCATTACCGTCCAAGACTAGAGATTCAGATGCCCTGAA
CTCCTCTTCAAGCCTGCCTTCATCGGTAAGGAATTCCCTGGTATCCACGAACTTACCTTC
AACTCTATCATGAAGTGTGATGTCGATGTCAGAAAGGATCTTTACAACAACATCGTCCTC
TCTGGTGGTACCACTATGTTCCCTGGTATTGCTGAAAGACTTTCTAAGGAAGTTAGCGCT
CTTGCTCCTTCTTCCATGAAGATTAAGGTTGTTGCTCCTCCTGAGAGAAGATACTCTGTC
TGGATTGGTGGTTCCATTCTTTCCTCTCTTTCTACCTTCCAAACTATGTGGATTACTAAG
GCTGAATATGATGAATCTGGTCCTTCCATCGTCCACAGAAAGTGCTTCTGA

>TTHERM_00190950(protein)
MAESESPAIVIDNGSGMCKAGIAGDDAPRAAFPSIVGRPKMPGIMVGMDQKECYVGEEAQ
AKRGVLNLKYPIEHGIVTDYDDMEKIWHHCFYNELRVTPEEHPCLLTEAPQNPKLNREKM
TKTMFETFNVPSFYVAIQAVLSLYASGRTTGIVVDSGDGVTHTVPIYEGYALPHAILRID
LAGRDLTEYCMKLLYEIGLNFSSTAEREIIRDIKEKLCYVAIDYEAELKAYKESSQNDKS
YELPDGNTITVQDQRFRCPELLFKPAFIGKEFPGIHELTFNSIMKCDVDVRKDLYNNIVL
SGGTTMFPGIAERLSKEVSALAPSSMKIKVVAPPERRYSVWIGGSILSSLSTFQTMWITK
AEYDESGPSIVHRKCF

>TTHERM_00190950(gene)
ATGGCTGAAAGTGAATCCCCCGCTATTGTTATTGATAACGGTTCCGGTATGTGCAAGGCT
GGTATTGCTGGTGACGATGCTCCCAGAGCCGCCTTCCCCTCCATTGTCGGTAGACCCAAA
ATGCCTGGTATCATGGTCGGTATGGATCAAAAGGAATGCTACGTTGGTGAAGAAGCCCAA
GCCAAGAGAGGTGTTTTGAATTTGAAATATCCCATTGAACACGGTATTGTCACCGATTAC
GATGATATGGAAAAGATCTGGCATCACTGCTTCTACAATGAACTCAGAGTTACCCCTGAA
GAACACCCCTGCTTACTTACTGAAGCTCCCCAAAACCCCAAGTTGAACAGAGAAAAGATG
ACCAAGACCATGTTCGAAACTTTCAACGTTCCCTCCTTCTACGTTGCCATCCAAGCCGTT
CTTTCCCTCTACGCTTCCGGTAGAACCACTGGTATCGTCGTTGACTCTGGTGATGGTGTT
ACCCACACTGTTCCCATCTATGAAGGTTACGCTCTTCCTCACGCCATCTTGAGAATCGAT
TTGGCTGGTAGAGACTTAACTGAATACTGCATGAAGCTCCTCTACGAAATTGGTTTGAAC
TTCTCTTCCACTGCCGAAAGAGAAATCATCAGAGATATCAAGGAAAAGCTCTGCTACGTC
GCTATCGACTACGAAGCTGAATTAAAGGCTTACAAGGAATCCTCCTAAAACGATAAATCT
TACGAATTACCCGATGGTAACACCATTACCGTCCAAGACTAGAGATTCAGATGCCCTGAA
CTCCTCTTCAAGCCTGCCTTCATCGGTAAGGAATTCCCTGGTATCCACGAACTTACCTTC
AACTCTATCATGAAGTGTGATGTCGATGTCAGAAAGGATCTTTACAACAACATCGTCCTC
TCTGGTGGTACCACTATGTTCCCTGGTATTGCTGAAAGACTTTCTAAGGAAGTTAGCGCT
CTTGCTCCTTCTTCCATGAAGATTAAGGTTGTTGCTCCTCCTGAGAGAAGATACTCTGTC
TGGATTGGTGGTTCCATTCTTTCCTCTCTTTCTACCTTCCAAACTATGTGGATTACTAAG
GCTGAATATGATGAATCTGGTCCTTCCATCGTCCACAGAAAGTGCTTCTGA